EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.1.9 | expressed in Escherichia coli ER2566 cells | Drosophila melanogaster |
1.8.1.9 | expressed in Escherichia coli ER2566 cells | Mus musculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.9 | 0.22 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in 50 mM potassium phosphate at pH 7.0 | Drosophila melanogaster | |
1.8.1.9 | 0.41 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in 50 mM potassium phosphate at pH 7.0 | Caenorhabditis elegans | |
1.8.1.9 | 0.45 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in 50 mM potassium phosphate at pH 7.0 | Mus musculus | |
1.8.1.9 | 0.53 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0 | Caenorhabditis elegans | |
1.8.1.9 | 0.83 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0 | Mus musculus | |
1.8.1.9 | 4.1 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0 | Drosophila melanogaster |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.8.1.9 | cytosol | - |
Rattus norvegicus | 5829 | - |
1.8.1.9 | mitochondrion | - |
Mus musculus | 5739 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.9 | selenium | the presence of a selenium atom is not chemically required to catalyze the reduction of the disulfide bond of thioredoxin | Drosophila melanogaster | |
1.8.1.9 | selenium | the presence of a selenium atom is not chemically required to catalyze the reduction of the disulfide bond of thioredoxin | Mus musculus | |
1.8.1.9 | selenium | the presence of a selenium atom is not chemically required to catalyze the reduction of the disulfide bond of thioredoxin | Rattus norvegicus | |
1.8.1.9 | selenium | the presence of a selenium atom is not chemically required to catalyze the reduction of the disulfide bond of thioredoxin | Caenorhabditis elegans | |
1.8.1.9 | selenium | the presence of a selenium atom is not chemically required to catalyze the reduction of the disulfide bond of thioredoxin | Anopheles gambiae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.1.9 | 55000 | - |
12% reducing SDS-PAGE | Drosophila melanogaster |
1.8.1.9 | 55000 | - |
12% reducing SDS-PAGE | Mus musculus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.9 | thioredoxin + NADP+ | Drosophila melanogaster | - |
thioredoxin disulfide + NADPH + H+ | - |
? | |
1.8.1.9 | thioredoxin + NADP+ | Mus musculus | - |
thioredoxin disulfide + NADPH + H+ | - |
? | |
1.8.1.9 | thioredoxin + NADP+ | Rattus norvegicus | - |
thioredoxin disulfide + NADPH + H+ | - |
? | |
1.8.1.9 | thioredoxin + NADP+ | Caenorhabditis elegans | - |
thioredoxin disulfide + NADPH + H+ | - |
? | |
1.8.1.9 | thioredoxin + NADP+ | Anopheles gambiae | - |
thioredoxin disulfide + NADPH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.1.9 | Anopheles gambiae | - |
- |
- |
1.8.1.9 | Caenorhabditis elegans | - |
- |
- |
1.8.1.9 | Drosophila melanogaster | - |
- |
- |
1.8.1.9 | Mus musculus | - |
- |
- |
1.8.1.9 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.1.9 | DEAE column column chromatography, 2',5'-ADP Sepharose 4B column chromatography, phenyl Sepharose column chromatography, and Ni-NTA agarose column chromatography | Drosophila melanogaster |
1.8.1.9 | DEAE column column chromatography, 2',5'-ADP Sepharose 4B column chromatography, phenyl Sepharose column chromatography, and Ni-NTA agarose column chromatography | Mus musculus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.9 | 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Drosophila melanogaster | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
1.8.1.9 | 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Mus musculus | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
1.8.1.9 | 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Rattus norvegicus | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
1.8.1.9 | 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Caenorhabditis elegans | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
1.8.1.9 | 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Anopheles gambiae | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
1.8.1.9 | thioredoxin + NADP+ | - |
Drosophila melanogaster | thioredoxin disulfide + NADPH + H+ | - |
? | |
1.8.1.9 | thioredoxin + NADP+ | - |
Mus musculus | thioredoxin disulfide + NADPH + H+ | - |
? | |
1.8.1.9 | thioredoxin + NADP+ | - |
Rattus norvegicus | thioredoxin disulfide + NADPH + H+ | - |
? | |
1.8.1.9 | thioredoxin + NADP+ | - |
Caenorhabditis elegans | thioredoxin disulfide + NADPH + H+ | - |
? | |
1.8.1.9 | thioredoxin + NADP+ | - |
Anopheles gambiae | thioredoxin disulfide + NADPH + H+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.1.9 | thioredoxin reductase | - |
Drosophila melanogaster |
1.8.1.9 | thioredoxin reductase | - |
Mus musculus |
1.8.1.9 | thioredoxin reductase | - |
Rattus norvegicus |
1.8.1.9 | thioredoxin reductase | - |
Caenorhabditis elegans |
1.8.1.9 | thioredoxin reductase | - |
Anopheles gambiae |
1.8.1.9 | TR2 | - |
Caenorhabditis elegans |
1.8.1.9 | TR3 | - |
Mus musculus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.9 | 2.23 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in 50 mM potassium phosphate at pH 7.0 | Caenorhabditis elegans | |
1.8.1.9 | 2.53 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0 | Caenorhabditis elegans | |
1.8.1.9 | 2.62 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in 50 mM potassium phosphate at pH 7.0 | Drosophila melanogaster | |
1.8.1.9 | 20.85 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in 50 mM potassium phosphate at pH 7.0 | Mus musculus | |
1.8.1.9 | 21.6 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0 | Drosophila melanogaster | |
1.8.1.9 | 48.42 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0 | Mus musculus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.8.1.9 | 6.5 | - |
the truncated enzyme shows a pH optimum for the reduction of 5,5'-dithiobis(2-nitrobenzoic acid) at pH 6.5 | Mus musculus |
1.8.1.9 | 7.5 | - |
the truncated enzyme shows a pH optimum for the reduction of 5,5'-dithiobis(2-nitrobenzoic acid) at pH 7.5 | Drosophila melanogaster |
1.8.1.9 | 8 | 8.5 | - |
Drosophila melanogaster |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.9 | NADPH | - |
Drosophila melanogaster | |
1.8.1.9 | NADPH | - |
Mus musculus | |
1.8.1.9 | NADPH | - |
Rattus norvegicus | |
1.8.1.9 | NADPH | - |
Caenorhabditis elegans | |
1.8.1.9 | NADPH | - |
Anopheles gambiae |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.9 | 0.22 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in 50 mM potassium phosphate at pH 7.0 | Drosophila melanogaster | |
1.8.1.9 | 4.78 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0 | Caenorhabditis elegans | |
1.8.1.9 | 5.27 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0 | Drosophila melanogaster | |
1.8.1.9 | 5.45 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in 50 mM potassium phosphate at pH 7.0 | Caenorhabditis elegans | |
1.8.1.9 | 45.33 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in 50 mM potassium phosphate at pH 7.0 | Mus musculus | |
1.8.1.9 | 58.33 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0 | Mus musculus |