Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fox, D.T.; Hotta, K.; Kim, C.Y.; Koppisch, A.T.
    The missing link in petrobactin biosynthesis: asbF encodes a (-)-3-dehydroshikimate dehydratase (2008), Biochemistry, 47, 12251-12253.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.118 expression in Escherichia coli Bacillus thuringiensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.118 EDTA 1 mM, complete loss of activity. More than 95% of activity can be restored in presence of Mg2+ Bacillus thuringiensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.118 0.125
-
 (-)-3-dehydroshikimate 37°C Bacillus thuringiensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.118 Co2+
-
 Bacillus thuringiensis
4.2.1.118 Mg2+ presence of Mg2+ restores activity after incubation with EDTA Bacillus thuringiensis
4.2.1.118 Mn2+
-
 Bacillus thuringiensis
4.2.1.118 additional information a divalent metal ion is absolutely required Bacillus thuringiensis

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.118 Bacillus thuringiensis
-
-
-
4.2.1.118 Bacillus thuringiensis 97-27
-
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
4.2.1.118 complete loss of activity in presence of EDTA. More than 95% of activity can be restored in presence of Mg2+ Bacillus thuringiensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.118 (-)-3-dehydroshikimate
-
 Bacillus thuringiensis 3,4-dihydroxybenzoate + H2O
-
 ?
4.2.1.118 (-)-3-dehydroshikimate
-
 Bacillus thuringiensis 97-27 3,4-dihydroxybenzoate + H2O
-
 ?
4.2.1.118 additional information enzyme displays to ionizable groups with pKa1 7.9, and pKa2 9.3 Bacillus thuringiensis ?
-
 ?
4.2.1.118 additional information enzyme displays to ionizable groups with pKa1 7.9, and pKa2 9.3 Bacillus thuringiensis 97-27 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.118 (-)-3-dehydroshikimate dehydratase
-
 Bacillus thuringiensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.118 46
-
 half-life 10 min Bacillus thuringiensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.118 3.8
-
 (-)-3-dehydroshikimate 37°C Bacillus thuringiensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.118 8.4 8.8
-
 Bacillus thuringiensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.118 7.4
-
 less than 15% residual activity Bacillus thuringiensis