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Literature summary extracted from

  • Graupner, M.; Xu, H.; White, R.H.
    Characterization of the 2-phospho-L-lactate transferase enzyme involved in coenzyme F(420) biosynthesis in Methanococcus jannaschii (2002), Biochemistry, 41, 3754-3761.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.28 expression in Escherichia coli Methanocaldococcus jannaschii

Protein Variants

EC Number Protein Variants Comment Organism
2.7.8.28 S211A mutant enzyme has the same specific activity as the wild type enzyme Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.28 EDTA
-
 Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.28 0.017
-
 lactyl-2-diphospho-5'-guanosine pH 7.0, 37°C Methanocaldococcus jannaschii
2.7.8.28 0.032
-
 7,8-didemethyl-8-hydroxy-5-deazariboflavin pH 7.0, 37°C Methanocaldococcus jannaschii
2.7.8.28 0.515
-
 lactyl-2-diphospho-5'-adenosine pH 7.0, 37°C Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.28 Mg2+ isolated CofD enzyme is not activated by the addition of 0-4 mM MgCl2. The inactivation by EDTA can be completely reversed by the addition of excess Mg2+ but not by the addition of Zn2+ or Mn2+ Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.8.28 34400
-
 2 * 34400, SDS-PAGE Methanocaldococcus jannaschii
2.7.8.28 66700
-
 gel filtration Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.28 lactyl-2-diphospho-5'-guanosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin Methanocaldococcus jannaschii the reaction is the fourth step in the biosynthesis of coenzyme F420 guanosine 5'-phosphate + coenzyme F420-0 coenzyme F420-0 is coenzyme F420 without glutamic acid ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.28 Methanocaldococcus jannaschii Q58653
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.28 recombinant enzyme Methanocaldococcus jannaschii

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.8.28 (2S)-lactyl-2-diphospho-5'-guanosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin = GMP + coenzyme F420-0 coenzyme F420-0 is coenzyme F420 without glutamic acid Methanocaldococcus jannaschii
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.8.28 0.36
-
-
 Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.28 lactyl-2-diphospho-5'-adenosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin catalysis does not appear to proceed via a covalent intermediate Methanocaldococcus jannaschii adenosine 5'-phosphate + coenzyme F420-0 coenzyme F420-0 is coenzyme F420 without glutamic acid ?
2.7.8.28 lactyl-2-diphospho-5'-guanosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin the reaction is the fourth step in the biosynthesis of coenzyme F420 Methanocaldococcus jannaschii guanosine 5'-phosphate + coenzyme F420-0 coenzyme F420-0 is coenzyme F420 without glutamic acid ?
2.7.8.28 lactyl-2-diphospho-5'-guanosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin catalysis does not appear to proceed via a covalent intermediate. Lactyl-2-diphospho-5'-guanosine is the preferred substrate. The substrate binding site of CofD is very specific for 7,8-didemethyl-8-hydroxy-5-deazariboflavin Methanocaldococcus jannaschii guanosine 5'-phosphate + coenzyme F420-0 coenzyme F420-0 is coenzyme F420 without glutamic acid ?
2.7.8.28 additional information to a lesser extent CofD also catalyzes a number of additional reactions that include the formation of 7,8-didemethyl-8-hydroxy-5-deazariboflavin 5'-phosphate, when the enzyme is incubated with 7,8-didemethyl-8-hydroxy-5-deazariboflavin and GDP, GTP, diphosphate, or tripolyphosphate, and the hydrolysis of F420-0 (F420 without glutamic acids) to 7,8-didemethyl-8-hydroxy-5-deazariboflavin Methanocaldococcus jannaschii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.8.28 dimer 2 * 34400, SDS-PAGE Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
2.7.8.28 2-phospho-L-lactate transferase
-
 Methanocaldococcus jannaschii
2.7.8.28 CofD
-
 Methanocaldococcus jannaschii
2.7.8.28 lactyl-2-diphospho-(5')guanosine:7,8-didemethyl-8-hydroxy-5-deazariboflavin 2-phospho-L-lactate transferase
-
 Methanocaldococcus jannaschii
2.7.8.28 lactyl-2-diphospho-(5')guanosine:Fo 2-phospho-L-lactate transferase
-
 Methanocaldococcus jannaschii
2.7.8.28 LPPG:7,8-didemethyl-8-hydroxy-5-deazariboflavin 2-phospho-L-lactate transferase
-
 Methanocaldococcus jannaschii
2.7.8.28 LPPG:Fo 2-phospho-L-lactate transferase
-
 Methanocaldococcus jannaschii
2.7.8.28 MJ1256
-
 Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.8.28 37
-
 assay at Methanocaldococcus jannaschii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.8.28 80
-
 24 h, completely stable Methanocaldococcus jannaschii
2.7.8.28 110
-
 30 min, complete inactivation Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.28 7
-
 assay at Methanocaldococcus jannaschii