EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.21 | native and mutated cDNA sequences cloned into vector pPICZalphaB and expressed in Pichia pastoris | Arabidopsis thaliana |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.21 | gluconolactone | competitively inhibits with p-nitrophenyl-beta-D-glucopyranoside, o-nitrophenyl-beta-D-glucopyranoside, and 4-methylumbelliferyl-beta-D-glucopyranoside as substrates | Arabidopsis thaliana | |
3.2.1.21 | additional information | the enzyme is very tolerant to glucose inhibition | Arabidopsis thaliana | |
3.2.1.21 | p-nitrophenyl-1-thio-beta-D-glucopyranoside | competitively inhibits with p-nitrophenyl-beta-D-glucopyranoside, o-nitrophenyl-beta-D-glucopyranoside, and 4-methylumbelliferyl-beta-D-glucopyranoside as substrates | Arabidopsis thaliana |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 0.78 | - |
4-methylumbelliferyl-beta-D-glucopyranoside | - |
Arabidopsis thaliana | |
3.2.1.21 | 1.9 | - |
p-nitrophenyl-beta-D-glucopyranoside | - |
Arabidopsis thaliana | |
3.2.1.21 | 2.1 | - |
o-nitrophenyl-beta-D-glucopyranoside | - |
Arabidopsis thaliana |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 60000 | - |
x * 60000, SDS-PAGE, recombinant mutated beta-glucosidase | Arabidopsis thaliana |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.21 | Arabidopsis thaliana | Q9SLA0 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.21 | recombinant beta-glucosidase purified by gel filtration to apparent homogeneity, 21fold with an overall enzyme yield of 6.8% | Arabidopsis thaliana |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.21 | seedling | - |
Arabidopsis thaliana | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 20.34 | - |
21fold purified recombinant beta-glucosidase | Arabidopsis thaliana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.21 | 2-nitrophenyl beta-D-glucopyranoside + H2O | 95% relative activity | Arabidopsis thaliana | 2-nitrophenol + D-glucopyranose | - |
? | |
3.2.1.21 | 4-methylumbelliferyl-beta-D-glucopyranoside + H2O | 163% relative activity | Arabidopsis thaliana | 4-methylumbelliferol + beta-D-glucopyranose | - |
? | |
3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | 100% relative activity | Arabidopsis thaliana | 4-nitrophenol + D-glucopyranose | - |
? | |
3.2.1.21 | amygdalin + H2O | 48% relative activity | Arabidopsis thaliana | prunasin + D-glucose | - |
? | |
3.2.1.21 | beta-gentiobiose + H2O | 6.3% relative activity | Arabidopsis thaliana | ? | - |
? | |
3.2.1.21 | D(+)cellobiose + H2O | 6.3% relative activity | Arabidopsis thaliana | ? | - |
? | |
3.2.1.21 | additional information | necessity of the nucleophilic glutamic acid residue, located in the motif YITENG, for the substrate hydrolysis. The mutated recombinant beta-glucosidase isoenzyme shows no activity with p-nitrophenyl 1-thio-beta-D-glucopyranoside, p-nitrophenyl beta-D-mannopyranoside, p-nitrophenyl beta-D-galactopyranoside and arbutin | Arabidopsis thaliana | ? | - |
? | |
3.2.1.21 | n-decyl-beta-D-glucopyranoside + H2O | 11% relative activity | Arabidopsis thaliana | n-decanol + beta-D-glucopyranose | - |
? | |
3.2.1.21 | n-heptyl-beta-D-glucopyranoside + H2O | 17% relative activity | Arabidopsis thaliana | n-heptanol + beta-D-glucopyranose | - |
? | |
3.2.1.21 | n-octyl-beta-D-glucopyranoside + H2O | 7% relative activity | Arabidopsis thaliana | n-octanol + beta-D-glucopyranose | - |
? | |
3.2.1.21 | o-nitrophenyl-beta-D-fucopyranoside + H2O | 117% relative activity | Arabidopsis thaliana | o-nitrophenol + beta-D-fucopyranose | - |
? | |
3.2.1.21 | o-nitrophenyl-beta-D-galactopyranoside + H2O | 17% relative activity | Arabidopsis thaliana | o-nitrophenol + beta-D-galactopyranose | - |
? | |
3.2.1.21 | p-nitrophenyl-beta-D-fucopyranoside + H2O | 71% relative activity | Arabidopsis thaliana | p-nitrophenol + beta-D-fucopyranose | - |
? | |
3.2.1.21 | prunasin + H2O | 39% relative activity | Arabidopsis thaliana | D-mandelonitrile + beta-D-glucose | - |
? | |
3.2.1.21 | salicin + H2O | 4.4% relative activity | Arabidopsis thaliana | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.21 | ? | x * 60000, SDS-PAGE, recombinant mutated beta-glucosidase | Arabidopsis thaliana |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.21 | beta-D-glucoside glucohydrolase | - |
Arabidopsis thaliana |
3.2.1.21 | beta-glucosidase | - |
Arabidopsis thaliana |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 40 | - |
mutated recombinant beta-glucosidase | Arabidopsis thaliana |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 42 | - |
is fairly stable in 50 mM sodium acetate buffer, pH 5.6, at temperatures up to 42°C, while retaining only 48% of the original activity at 50°C for 10 min. It is completely inactivated upon incubation at 60°C for 10 min | Arabidopsis thaliana |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 5.6 | - |
recombinant mutated beta-glucosidase | Arabidopsis thaliana |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 5 | 8.5 | mutated recombinant beta-glucosidase is fairly stable at pH 5.0 and 8.5, exhibiting 60 and 62% activities, respectively. It retains over 50% of the original activity between pH 4-7.5. Between pH values 6.0 and 7.2, it retains over 98% activity after incubation at 37°C for 2 h | Arabidopsis thaliana |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 2 | - |
gluconolactone | with para-nitrophenyl-beta-D-glucopyranoside as substrate | Arabidopsis thaliana | |
3.2.1.21 | 2.2 | - |
gluconolactone | with ortho-nitrophenyl-beta-D-glucopyranoside as substrate | Arabidopsis thaliana | |
3.2.1.21 | 4.7 | - |
gluconolactone | with 4-methylumbelliferyl-beta-D-glucopyranoside as substrate | Arabidopsis thaliana |