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Literature summary extracted from

  • Claperon, C.; Rozenfeld, R.; Iturrioz, X.; Inguimbert, N.; Okada, M.; Roques, B.; Maigret, B.; Llorens-Cortes, C.
    Asp218 participates with Asp213 to bind a Ca2+ atom into the S1 subsite of aminopeptidase A: a key element for substrate specificity (2008), Biochem. J., 416, 37-46.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.7 expressed in CHO-K1 cells Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.11.7 additional information substitution mutations at Asp218 and Asp213 maintain the ability of APA to be activated by Ca2+ and induce a decrease of the inhibitory potencies of inhibitors homologous with acidic residues Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.7 glutamate phosphonate specific aminopeptidase A inhibitor Mus musculus
3.4.11.7 N-(2-aminoethyl)-4-methyl-5-sulfanyl-pentanamide
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.7 0.152
-
 L-Glu-p-nitroanilide wild type enzyme, in absence of Ca2+, in 50 mM Tris/HCl buffer (pH 7.4), at 37°C Mus musculus
3.4.11.7 1.475
-
 L-Glu-p-nitroanilide wild type enzyme, in absence of Ca2+, in 50 mM Tris/HCl buffer (pH 7.4), at 37°C Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.7 membrane
-
 Mus musculus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.7 Ca2+ each APA monomer contains one Ca2+ atom, Ca2+ (from 0 to 4 mM) increases the activity of the wild type enzyme Mus musculus
3.4.11.7 Zn2+ contains zinc Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.7 140000
-
 minor immature form, SDS-PAGE Mus musculus
3.4.11.7 160000
-
 major mature form, SDS-PAGE Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.7 Mus musculus P16406
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.7 Talon Co2+ column chromatography Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.7 angiotensin II + H2O
-
 Mus musculus aspartic acid + angiotensin III
-
 ?
3.4.11.7 L-Glu-p-nitroanilide + H2O
-
 Mus musculus L-Glu + p-nitroaniline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.7 aminopeptidase A
-
 Mus musculus
3.4.11.7 APA
-
 Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.7 295
-
 L-Glu-p-nitroanilide wild type enzyme, in absence of Ca2+, in 50 mM Tris/HCl buffer (pH 7.4), at 37°C Mus musculus
3.4.11.7 720
-
 L-Glu-p-nitroanilide wild type enzyme, in absence of Ca2+, in 50 mM Tris/HCl buffer (pH 7.4), at 37°C Mus musculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.11.7 0.00007
-
 N-(2-aminoethyl)-4-methyl-5-sulfanyl-pentanamide wild type enzyme, in 50 mM Tris/HCl buffer (pH 7.4), 4 mM Ca2+, at 37°C Mus musculus