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Literature summary extracted from

  • Dong, X.; Fu, M.; Sun, Y.
    Refolding of recombinant homodimeric malate dehydrogenase expressed in Escherichia coli as inclusion bodies (2008), Biochem. Eng. J., 38, 341-348.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.37 expressed in Escherichia coli as inclusion bodies Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.37 mitochondrion
-
 Sus scrofa 5739
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.37 Sus scrofa
-
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.1.1.37 glycerol, sucrose and adenosine triphosphate are effective folding aids, refolding buffer consists of 50 mM Tris-HCl (pH 7.6), 5 mM dithiothreitol, 0.32 mM urea and an additive at a predetermined concentration, such as glycerol (3.2 M), sucrose (1 M), arginine (1 M), glycine (1 M), acetamide (1 M), and ATP (2 mM), refolding at low temperature (4°C) enhances the refolding yield of the homodimeric enzyme. Artificial chaperone-assisted refolding buffer consists of 50 mM Tris-HCl (pH 7.6), 5 mM dithiothreitol, 0.32 M urea, 1.2 (or 0.6) mM cetyltrimethylammonium bromide, and 4.8 mM beta-cyclodextrin. Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.37 heart
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.37 (S)-malate + NAD+
-
 Sus scrofa oxaloacetate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.37 homodimer x-ray crystallography Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
1.1.1.37 MDH
-
 Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.37 NAD+
-
 Sus scrofa