EC Number | Cloned (Comment) | Organism |
---|---|---|
1.6.5.10 | overexpression of CBR4 in bovine endothelial cells reveals that the enzyme has a non-cleavable mitochondrial targeting signal | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.6.5.10 | Cibacron blue F3GA | the 1,4-naphthoquinone activity of CBR4 is potently inhibited, competitive with respect to NADPH | Homo sapiens | |
1.6.5.10 | additional information | the enzyme is insensitive to dicuomarol and quercetin, potent inhibitors of cytosolic quinone reductases | Homo sapiens | |
1.6.5.10 | NADP+ | the 1,4-naphthoquinone activity of CBR4 is potently inhibited, competitive with respect to NADPH | Homo sapiens | |
1.6.5.10 | Procion red HE3B | the 1,4-naphthoquinone activity of CBR4 is potently inhibited, competitive with respect to NADPH | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.6.5.10 | 0.0016 | - |
9,10-phenanthrenequinone | pH 7.4, 25°C | Homo sapiens | |
1.6.5.10 | 0.0019 | - |
1,4-benzoquinone | pH 7.4, 25°C | Homo sapiens | |
1.6.5.10 | 0.003 | - |
1,2-naphthoquinone | pH 7.4, 25°C | Homo sapiens | |
1.6.5.10 | 0.003 | - |
2-methyl-1,4-naphthoquinone | pH 7.4, 25°C | Homo sapiens | |
1.6.5.10 | 0.0043 | - |
5-Hydroxy-2-methyl-1,4-naphthoquinone | pH 7.4, 25°C | Homo sapiens | |
1.6.5.10 | 0.0044 | - |
2,3-dimethoxy-5-methyl-1,4-benzoquinone | pH 7.4, 25°C | Homo sapiens | |
1.6.5.10 | 0.0045 | - |
5-hydroxy-1,4-naphthoquinone | pH 7.4, 25°C | Homo sapiens | |
1.6.5.10 | 0.0065 | - |
1,4-Naphthoquinone | pH 7.4, 25°C | Homo sapiens | |
1.6.5.10 | 0.036 | - |
menadione | pH 7.4, 25°C | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.6.5.10 | mitochondrion | CBR4 is a mitochondrial matrix protein, its N-terminal signal is not cleaved | Homo sapiens | 5739 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.6.5.10 | 25000 | - |
4 * 25000, SDS-PAGE | Homo sapiens |
1.6.5.10 | 100000 | - |
gel filtration | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.6.5.10 | Homo sapiens | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.6.5.10 | A-549 cell | - |
Homo sapiens | - |
1.6.5.10 | Caco-2 cell | - |
Homo sapiens | - |
1.6.5.10 | kidney | - |
Homo sapiens | - |
1.6.5.10 | liver | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.6.5.10 | 1,2-naphthoquinone + NADPH + H+ | - |
Homo sapiens | 1,2-naphthoquinol + NADP+ | - |
? | |
1.6.5.10 | 1,4-benzoquinone + NADPH + H+ | 1,4-benzoquinone is oxidized almost stoichiometric amount of NADPH, suggesting that it is reduced to its hydroquinone with a two-electron reduction mechanism | Homo sapiens | 1,4-benzoquinol + NADP+ | - |
? | |
1.6.5.10 | 1,4-naphthoquinone + NADPH + H+ | 1,4-naphthoquinone participates in the redox cycling in their reduction by this enzyme as evidenced by excess NADPH oxidation over quinone reduction. CBR4 may reduce 1,4-naphthoquinone in the two-electron reduction mechanism, but produces reactive superoxide and semiquinones through their redox cycling | Homo sapiens | 1,4-naphthoquinol + NADP+ | - |
? | |
1.6.5.10 | 2,3-dimethoxy-5-methyl-1,4-benzoquinone + NADPH + H+ | - |
Homo sapiens | 2,3-dimethoxy-5-methyl-1,4-benzoquinol + NADP+ | - |
? | |
1.6.5.10 | 2-methyl-1,4-naphthoquinone + NADPH + H+ | - |
Homo sapiens | 2-methyl-1,4-naphthoquinol + NADP+ | - |
? | |
1.6.5.10 | 5-hydroxy-1,4-naphthoquinone + NADPH + H+ | 5-hydroxy-1,4-naphthoquinone participates in the redox cycling in their reduction by this enzyme as evidenced by excess NADPH oxidation over quinone reduction | Homo sapiens | 5-hydroxy-1,4-naphthoquinol + NADP+ | - |
? | |
1.6.5.10 | 5-hydroxy-2-methyl-1,4-naphthoquinone + NADPH + H+ | - |
Homo sapiens | 5-hydroxy-2-methyl-1,4-naphthoquinol + NADP+ | - |
? | |
1.6.5.10 | 9,10-phenanthrenequinone + NADPH + H+ | 9,10-phenanthrenequinone participates in the redox cycling in their reduction by this enzyme as evidenced by excess NADPH oxidation over quinone reduction. CBR4 may reduce 9,10-phenanthrenequinone in the two-electron reduction mechanism, but produces reactive superoxide and semiquinones through their redox cycling | Homo sapiens | 9,10-phenanthrenequinol + NADP+ | - |
? | |
1.6.5.10 | menadione + NADPH + H+ | - |
Homo sapiens | menadiol + NADP+ | - |
? | |
1.6.5.10 | additional information | the enzyme exhibits NADPH-dependent reductase activity for o- and p-quinones, but not for other aldehydes and ketones. In vitro quinone reduction by CBR4 generates superoxide through the redox cycling, and suggest that the enzyme may be involved in the induction of apoptosis by cytotoxic 9,10-phenanthrenequinone | Homo sapiens | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.6.5.10 | tetramer | 4 * 25000, SDS-PAGE | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.6.5.10 | CBR4 | - |
Homo sapiens |
1.6.5.10 | NADPH-dependent quinone reductase | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.6.5.10 | 25 | - |
assay at | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.6.5.10 | 0.068 | - |
2,3-dimethoxy-5-methyl-1,4-benzoquinone | pH 7.4, 25°C | Homo sapiens | |
1.6.5.10 | 0.19 | - |
5-hydroxy-1,4-naphthoquinone | pH 7.4, 25°C | Homo sapiens | |
1.6.5.10 | 0.195 | - |
1,4-Naphthoquinone | pH 7.4, 25°C | Homo sapiens | |
1.6.5.10 | 0.207 | - |
1,4-benzoquinone | pH 7.4, 25°C | Homo sapiens | |
1.6.5.10 | 0.21 | - |
1,2-naphthoquinone | pH 7.4, 25°C | Homo sapiens | |
1.6.5.10 | 0.212 | - |
menadione | pH 7.4, 25°C | Homo sapiens | |
1.6.5.10 | 0.305 | - |
9,10-phenanthrenequinone | pH 7.4, 25°C | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.6.5.10 | 6.5 | - |
- |
Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.6.5.10 | NADPH | - |
Homo sapiens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.6.5.10 | 0.0003 | - |
Procion red HE3B | pH 7.4, 25°C | Homo sapiens | |
1.6.5.10 | 0.001 | - |
Cibacron blue F3GA | pH 7.4, 25°C | Homo sapiens | |
1.6.5.10 | 0.057 | - |
NADP+ | pH 7.4, 25°C | Homo sapiens |