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Literature summary extracted from

  • Lisurek, M.; Kang, M.J.; Hartmann, R.W.; Bernhardt, R.
    Identification of monohydroxy progesterones produced by CYP106A2 using comparative HPLC and electrospray ionisation collision-induced dissociation mass spectrometry (2004), Biochem. Biophys. Res. Commun., 319, 677-682.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.8 expression in Escherichia coli Priestia megaterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.8 0.251
-
progesterone pH 7.4, 30°C, for determination of Km-value the formation of the product 15beta-hydroxy-progesterone is determined Priestia megaterium

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.8 Priestia megaterium Q06069 ATCC 13368, recombinantly expressed in Escherichia coli
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Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.8
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Priestia megaterium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.15.8 additional information
-
the Vmax values for 15beta-hydroxyprogesterone, 6beta-hydroxyprogesterone, 11alpha-hydroxyprogesterone, and 9alpha-hydroxyprogesterone are determined as 337.3, 22.3, 17.5, and 6.5 nmol product/min/nmol CYP106A2 Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.8 progesterone + reduced adrenodoxin + O2 identification of monohydroxy progesterones using comparative HPLC and electrospray ionisation collision-induced dissociation mass spectrometry. 15beta-Hydroxy-progesterone is the main product. 6beta-Hydroxyprogesterone, 11alpha-hydroxyprogesterone, and 9alpha-hydroxyprogesterone are formed at small amounts Priestia megaterium 15beta-hydroxyprogesterone + oxidized adrenodoxin + H2O
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Synonyms

EC Number Synonyms Comment Organism
1.14.15.8 CYP106A2
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Priestia megaterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.8 30
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assay at Priestia megaterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.8 7.4
-
assay at Priestia megaterium