Literature summary extracted from
Lisurek, M.; Kang, M.J.; Hartmann, R.W.; Bernhardt, R.
Identification of monohydroxy progesterones produced by CYP106A2 using comparative HPLC and electrospray ionisation collision-induced dissociation mass spectrometry (2004), Biochem. Biophys. Res. Commun., 319, 677-682.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.14.15.8 |
expression in Escherichia coli |
Priestia megaterium |
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
1.14.15.8 |
0.251 |
- |
progesterone |
pH 7.4, 30°C, for determination of Km-value the formation of the product 15beta-hydroxy-progesterone is determined |
Priestia megaterium |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.15.8 |
Priestia megaterium |
Q06069 |
ATCC 13368, recombinantly expressed in Escherichia coli |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.14.15.8 |
- |
Priestia megaterium |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
1.14.15.8 |
additional information |
- |
the Vmax values for 15beta-hydroxyprogesterone, 6beta-hydroxyprogesterone, 11alpha-hydroxyprogesterone, and 9alpha-hydroxyprogesterone are determined as 337.3, 22.3, 17.5, and 6.5 nmol product/min/nmol CYP106A2 |
Priestia megaterium |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.15.8 |
progesterone + reduced adrenodoxin + O2 |
identification of monohydroxy progesterones using comparative HPLC and electrospray ionisation collision-induced dissociation mass spectrometry. 15beta-Hydroxy-progesterone is the main product. 6beta-Hydroxyprogesterone, 11alpha-hydroxyprogesterone, and 9alpha-hydroxyprogesterone are formed at small amounts |
Priestia megaterium |
15beta-hydroxyprogesterone + oxidized adrenodoxin + H2O |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.14.15.8 |
CYP106A2 |
- |
Priestia megaterium |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
1.14.15.8 |
30 |
- |
assay at |
Priestia megaterium |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.14.15.8 |
7.4 |
- |
assay at |
Priestia megaterium |