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Literature summary extracted from

  • Boisrame, A.; Gaillardin, C.
    Heterologous expression and characterization of a beta-1,6-glucanase from Aspergillus fumigatus (2009), Appl. Microbiol. Biotechnol., 82, 663-669.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.75 into expression/secretion vector pINA1296+FLAG, expressed in Yarrowia lipolytica strain Po1g: MatA, leu2-270, ura3-302::pBR322-URA3, xpr2-322, axp-2, which contains an integrated pBR322 plasmid as a docking platform Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.75 50000
-
-
 Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.75 Aspergillus fumigatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.75 FLAG-tagged Neg1 protein purified by batch adsorption Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.75 laminarin + H2O only 2% of activity at 45°C Aspergillus fumigatus ?
-
 ?
3.2.1.75 additional information is active on Candida albicans cell walls, is able to release a GPI-anchored cell wall protein linked to the beta-1,6-glucan polymer through a remnant of its anchor. No activity with beta-1,3-glucan, dextran and amylose Aspergillus fumigatus ?
-
 ?
3.2.1.75 pustulan + H2O
-
 Aspergillus fumigatus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.75 beta-1,6-glucanase
-
 Aspergillus fumigatus
3.2.1.75 Neg1
-
 Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.75 4.5
-
-
 Aspergillus fumigatus