EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.2 | industry | possible uses of this enzyme in the production of wax esters | Marinobacter nauticus VT8 |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.2 | expressed in Escherichia coli strain TB1 as a fusion protein with the maltose binding protein on the N-terminus | Marinobacter nauticus VT8 |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.2 | ascorbic acid | in 100 mM MES buffer (pH 6.5) with 200 mM NADPH, 0.25 mM decanal, and 125 microg of FALDR fusion protein. 1 mM inhibits by 24% | Marinobacter nauticus VT8 | |
1.1.1.2 | beta-mercaptoethanol | in 100 mM MES buffer (pH 6.5) with 200 mM NADPH, 0.25 mM decanal, and 125 microg of FALDR fusion protein. 1 mM inhibits by 25% | Marinobacter nauticus VT8 | |
1.1.1.2 | diethyldithiocarbamate | in 100 mM MES buffer (pH 6.5) with 200 mM NADPH, 0.25 mM decanal, and 125 microg of FALDR fusion protein. 1 mM inhibits by 50%, 0.25 mM inhibits by 39% | Marinobacter nauticus VT8 | |
1.1.1.2 | dipyridyl | in 100 mM MES buffer (pH 6.5) with 200 mM NADPH, 0.25 mM decanal, and 125 microg of FALDR fusion protein. 1 mM inhibits by 54%, 0.25 mM inhibits by 41% | Marinobacter nauticus VT8 | |
1.1.1.2 | Dithionite | in 100 mM MES buffer (pH 6.5) with 200 mM NADPH, 0.25 mM decanal, and 125 microg of FALDR fusion protein. 1 mM inhibits by 75%, 0.25 mM inhibits by 92% | Marinobacter nauticus VT8 | |
1.1.1.2 | dithiothreitol | in 100 mM MES buffer (pH 6.5) with 200 mM NADPH, 0.25 mM decanal, and 125 microg of FALDR fusion protein. 1 mM inhibits by 24% | Marinobacter nauticus VT8 | |
1.1.1.2 | Dodecanol | in 100 mM MES buffer (pH 6.5) with 200 mM NADPH, 0.25 mM decanal, and 125 microg of FALDR fusion protein. 1 mM inhibits by 46%, 0.25 mM inhibits by 45% | Marinobacter nauticus VT8 | |
1.1.1.2 | EDTA | in 100 mM MES buffer (pH 6.5) with 200 mM NADPH, 0.25 mM decanal, and 125 microg of FALDR fusion protein. 1 mM inhibits by 19% | Marinobacter nauticus VT8 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.2 | 0.177 | - |
cis-11-hexadecenal | - |
Marinobacter nauticus VT8 |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 57000 | - |
1 * 57000, SDS-PAGE | Marinobacter nauticus VT8 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.2 | Marinobacter nauticus VT8 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.2 | to near homogeneity by amylose affinity chromatography | Marinobacter nauticus VT8 |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 0.085 | - |
highest specific activity for the reduction of decanal | Marinobacter nauticus VT8 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.2 | cis-11-hexadecenal + NADPH | 87% relative activity | Marinobacter nauticus VT8 | cis-11-hexadecenol + NADP+ | - |
ir | |
1.1.1.2 | decanal + NADPH | no detectable catalysis of the reverse reaction, the oxidation of decanol by NADP+. 100% relative activity | Marinobacter nauticus VT8 | decanol + NADP+ | - |
ir | |
1.1.1.2 | dodecanal + NADPH | 55% relative activity | Marinobacter nauticus VT8 | dodecanol + NADP+ | - |
ir | |
1.1.1.2 | additional information | no activity with butanal, hexanal and benzaldehyde | Marinobacter nauticus VT8 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.2 | monomer | 1 * 57000, SDS-PAGE | Marinobacter nauticus VT8 |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.2 | NADPH-dependent FALDR | - |
Marinobacter nauticus VT8 |
1.1.1.2 | NADPH-dependent fatty aldehyde reductase | - |
Marinobacter nauticus VT8 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 6.3 | - |
- |
Marinobacter nauticus VT8 |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 6.3 | 8 | - |
Marinobacter nauticus VT8 |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.2 | NADP+ | - |
Marinobacter nauticus VT8 | |
1.1.1.2 | NADPH | - |
Marinobacter nauticus VT8 |