EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.5.9 | Urea | urea causes a dose-dependent but reversible inhibition by dissoziation of the hetero-oligomeric enzyme comparable with the effect of heat-treatment, 3.5 M urea reduces GDH activity by 20%, 5 M urea by 80% and 8 M urea by ca. 90% | Burkholderia cepacia |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.5.9 | 1.3 | - |
D-glucose | at 40°C after preincubation of the enzyme at 70°C for 30 min | Burkholderia cepacia | |
1.1.5.9 | 1.4 | - |
phenazine methosulfate | at 40°C | Burkholderia cepacia | |
1.1.5.9 | 2.5 | - |
D-glucose | at 40°C | Burkholderia cepacia | |
1.1.5.9 | 2.7 | - |
phenazine methosulfate | at 40°C after preincubation of the enzyme at 70°C for 30 min | Burkholderia cepacia |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.5.9 | 43000 | - |
SDS-PAGE, x * 67000, alpha-subunit, x * 43000, beta subunit, determined as cytochrome c via spectroscopic analyses | Burkholderia cepacia |
1.1.5.9 | 67000 | - |
SDS-PAGE, x * 67000, alpha-subunit, x * 43000, beta subunit, determined as cytochrome c via spectroscopic analyses | Burkholderia cepacia |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.5.9 | Burkholderia cepacia | - |
bacterial strain SM4 is used, isolated from a soil sample near a Japanese hot spring | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.5.9 | - |
Burkholderia cepacia |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.5.9 | additional information | - |
290 micromol/min/mg Vmax of oxidation of glucose at 40°C | Burkholderia cepacia |
1.1.5.9 | additional information | - |
4.3 micromol/min/mg Vmax of oxidation of phenazine methosulfate at 40°C after preincubation of the enzyme at 70°C for 30 min | Burkholderia cepacia |
1.1.5.9 | additional information | - |
470 micromol/min/mg Vmax of oxidation of phenazine methosulfate at 40°C | Burkholderia cepacia |
1.1.5.9 | additional information | - |
66 micromol/min/mg Vmax of oxidation of glucose at 40°C after preincubation of the enzyme at 70°C for 30 min | Burkholderia cepacia |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.5.9 | D-glucose + 2,6-dichlorophenolindophenol | - |
Burkholderia cepacia | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.5.9 | D-glucose + phenazine methosulfate | - |
Burkholderia cepacia | D-glucono-1,5-lactone + reduced phenazine methosulfate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.5.9 | heteromer | SDS-PAGE, x * 67000, alpha-subunit, x * 43000, beta subunit, determined as cytochrome c via spectroscopic analyses | Burkholderia cepacia |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.5.9 | GDH | - |
Burkholderia cepacia |
1.1.5.9 | glucose dehydrogenase | - |
Burkholderia cepacia |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.5.9 | 70 | - |
after preincubation with 5.8 M urea for 30 min at 25°C | Burkholderia cepacia |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.5.9 | 50 | 80 | after preincubation with 5.8 M urea for 30 min at 25°C | Burkholderia cepacia |