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Literature summary extracted from

  • Kouakou, T.H.; Due, E.A.; Kouadio, N.E.; Niamke, S.; Kouadio, Y.J.; Merillon, J.M.
    Purification and characterization of cell suspensions peroxidase from cotton (Gossypium hirsutum L.) (2008), Appl. Biochem. Biotechnol., 157, 575-592.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.7 ascorbic acid complete inhibition at 1 mM; complete inhibition at 1 mM; complete inhibition at 1 mM Gossypium hirsutum
1.11.1.7 beta-mercaptoethanol 94% inhibition at 1 mM; 94% inhibition at 1 mM; 96% inhibition at 1 mM; 96% inhibition at 1 mM Gossypium hirsutum
1.11.1.7 cysteine 73% inhibition at 1 mM; 73% inhibition at 1 mM; 78% inhibition at 1 mM; 78% inhibition at 1 mM Gossypium hirsutum
1.11.1.7 dithiothreitol 98% inhibition at 1 mM; 98% inhibition at 1 mM; complete inhibition at 1 mM; complete inhibition at 1 mM Gossypium hirsutum
1.11.1.7 EDTA 24% inhibition at 1 mM; 28% inhibition at 1 mM; 28% inhibition at 1 mM; 28% inhibition at 1 mM Gossypium hirsutum
1.11.1.7 Hg2+ 41% relative activity at 1 mM; 50% inhibition at 1 mM; 50% relative activity at 1 mM; 59% inhibition at 1 mM Gossypium hirsutum
1.11.1.7 K+ 26% inhibition at 1 mM; 27% inhibition at 1 mM; 74% relative activity at 1 mM; 77% relative activity at 1 mM Gossypium hirsutum
1.11.1.7 KI 30% inhibition at 1 mM; 30% inhibition at 1 mM; 36% inhibition at 1 mM; 36% inhibition at 1 mM Gossypium hirsutum
1.11.1.7 Mn2+ 30% inhibition at 1 mM; 30% inhibition at 1 mM; 70% relative activity at 1 mM; 73% relative activity at 1 mM Gossypium hirsutum
1.11.1.7 additional information not inhibited by Cu2+ and Mg2+ Gossypium hirsutum
1.11.1.7 Na+ 44% inhibition at 1 mM; 49% inhibition at 1 mM; 49% inhibition at 1 mM Gossypium hirsutum
1.11.1.7 NaCl 44% inhibition at 1 mM Gossypium hirsutum
1.11.1.7 Sodium metabisulfite complete inhibition at 1 mM; complete inhibition at 1 mM; complete inhibition at 1 mM Gossypium hirsutum
1.11.1.7 Tween 80 30% inhibition at 1 mM; 38% inhibition at 1 mM; 45% inhibition at 1 mM; 45% inhibition at 1 mM Gossypium hirsutum
1.11.1.7 Zn2+ 38% relative activity at 1 mM; 46% relative activity at 1 mM; 54% inhibition at 1 mM; 62% inhibition at 1 mM Gossypium hirsutum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.7 Al3+ 108% increased activity at 1 mM Gossypium hirsutum
1.11.1.7 Al3+ 77% increased activity at 1 mM Gossypium hirsutum
1.11.1.7 Al3+ 177% relative activity at 1 mM Gossypium hirsutum
1.11.1.7 Al3+ 208% relative activity at 1 mM Gossypium hirsutum
1.11.1.7 Ca2+ 40% increased activity at 1 mM Gossypium hirsutum
1.11.1.7 Ca2+ 51% increased activity at 1 mM Gossypium hirsutum
1.11.1.7 Ca2+ 140% relative activity at 1 mM Gossypium hirsutum
1.11.1.7 Ca2+ 151% relative activity at 1 mM Gossypium hirsutum
1.11.1.7 Fe3+ 193% increased activity at 1 mM Gossypium hirsutum
1.11.1.7 Fe3+ 205% increased activity at 1 mM Gossypium hirsutum
1.11.1.7 Fe3+ 293% relative activity at 1 mM Gossypium hirsutum
1.11.1.7 Fe3+ 305% relative activity at 1 mM Gossypium hirsutum
1.11.1.7 additional information not stimulated by Mg2+ and Cu2+ Gossypium hirsutum
1.11.1.7 Ni2+ 23% increased activity at 1 mM Gossypium hirsutum
1.11.1.7 Ni2+ 9% increased activity at 1 mM Gossypium hirsutum
1.11.1.7 Ni2+ 109% relative activity at 1 mM Gossypium hirsutum
1.11.1.7 Ni2+ 123% relative activity at 1 mM Gossypium hirsutum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.7 39100
-
 SDS-PAGE Gossypium hirsutum
1.11.1.7 64000
-
 SDS-PAGE Gossypium hirsutum

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.7 Gossypium hirsutum
-
 cultivar Coker 312
-
1.11.1.7 Gossypium hirsutum
-
 cultivar R405-2000
-
1.11.1.7 Gossypium hirsutum Q8RVP6 cultivar Coker 312
-
1.11.1.7 Gossypium hirsutum Q8RVW0 cultivar R405-2000
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.7 ammonium sulfate precipitation and Sephadex G-200 gel filtration Gossypium hirsutum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.7 callus
-
 Gossypium hirsutum
-
1.11.1.7 cell suspension culture
-
 Gossypium hirsutum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.7 4-methylcatechol + H2O2 28% activity compared to guaiacol Gossypium hirsutum ?
-
 ?
1.11.1.7 4-methylcatechol + H2O2 47% activity compared to guaiacol Gossypium hirsutum ?
-
 ?
1.11.1.7 benzoic acid + H2O2 10% activity compared to guaiacol Gossypium hirsutum ?
-
 ?
1.11.1.7 benzoic acid + H2O2 19% activity compared to guaiacol Gossypium hirsutum ?
-
 ?
1.11.1.7 catechin + H2O2 10% activity compared to guaiacol Gossypium hirsutum ?
-
 ?
1.11.1.7 catechin + H2O2 19% activity compared to guaiacol Gossypium hirsutum ?
-
 ?
1.11.1.7 catechol + H2O2 30% activity compared to guaiacol Gossypium hirsutum ?
-
 ?
1.11.1.7 catechol + H2O2 36% activity compared to guaiacol Gossypium hirsutum ?
-
 ?
1.11.1.7 chlorogenic acid + H2O2 80% activity compared to guaiacol Gossypium hirsutum ?
-
 ?
1.11.1.7 chlorogenic acid + H2O2 95% activity compared to guaiacol Gossypium hirsutum ?
-
 ?
1.11.1.7 guaiacol + H2O2 100% activity with guaiacol at 25 mM Gossypium hirsutum tetraguaiacol + H2O
-
 ?
1.11.1.7 guaiacol + H2O2 shows high efficiency of interaction with guaiacol at 25 mM Gossypium hirsutum tetraguaiacol + H2O
-
 ?
1.11.1.7 guaiacol + H2O2 100% activity, high efficiency of interaction with guaiacol at 25 mM Gossypium hirsutum tetraguaiacol + H2O
-
 ?
1.11.1.7 p-coumaric acid + H2O2 13% activity compared to guaiacol Gossypium hirsutum ?
-
 ?
1.11.1.7 p-coumaric acid + H2O2 15% activity compared to guaiacol Gossypium hirsutum ?
-
 ?
1.11.1.7 pyrogallol + H2O2 84% activity compared to guaiacol Gossypium hirsutum ?
-
 ?
1.11.1.7 pyrogallol + H2O2 90% activity compared to guaiacol Gossypium hirsutum ?
-
 ?
1.11.1.7 pyrogallol + H2O2 84% activity compared to guaiacol Gossypium hirsutum purpurogallin + H2O
-
 ?
1.11.1.7 pyrogallol + H2O2 90% activity compared to guaiacol Gossypium hirsutum purpurogallin + H2O
-
 ?
1.11.1.7 tetramethylbenzidine + H2O2 206% activity compared to guaiacol Gossypium hirsutum ?
-
 ?
1.11.1.7 tetramethylbenzidine + H2O2 215% activity compared to guaiacol Gossypium hirsutum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.7 cPOD-I
-
 Gossypium hirsutum
1.11.1.7 cPOD-I isozyme Gossypium hirsutum
1.11.1.7 POD
-
 Gossypium hirsutum
1.11.1.7 rPOD-II
-
 Gossypium hirsutum
1.11.1.7 rPOD-II isozyme Gossypium hirsutum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.7 25
-
-
 Gossypium hirsutum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.7 20 70 relatively stable at 20-30°C, a decrease in POD activity of 75%, 85%, and 90% is found after 10 min at 50°C, 60°C, and 70°C, respectively. The enzyme activity at 50°C, 60°C, and 70-80°C is completely exhausted in 40, 25, and 10 min, respectively. Gossypium hirsutum
1.11.1.7 20 80 relatively stable between 20-30°C, activity decreases by 20% at 40°C, the time required for 50% inactivation of activity at 50°C, 60°C, and 70°C is about 5 min, a decrease in POD activity of 75%, 85%, and 90% occurs after 10 min at 50°C, 60°C, and 70°C, respectively, the enzyme activity at 50°C, 60°C, and 70-80°C is completely exhausted in 40, 25, and 10 min Gossypium hirsutum
1.11.1.7 20 80 relatively stable between 20-30°C, activity decreases by 25% at 40°C, the time required for 50% inactivation of activity at 50°C, 60°C, and 70°C is about 5 min, a decrease in POD activity of 75%, 85%, and 90% occurs after 10 min at 50°C, 60°C, and 70°C, respectively, the enzyme activity at 50°C, 60°C, and 70-80°C is completely exhausted in 40, 25, and 10 min Gossypium hirsutum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.7 5
-
-
 Gossypium hirsutum
1.11.1.7 6
-
-
 Gossypium hirsutum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.7 4 8 the enzyme retains more than 90% of its initial activity within the pH range 6.0-7.0, weakest activity at pH 8.0, enzymatic activity is lowest after incubation for 60 min at pH 4.0 and 8.0 Gossypium hirsutum
1.11.1.7 5 8 the enzyme retains more than 90% of its initial activity within the pH range 5.0-6.0, weakest activity at pH 8.0, enzymatic activity is lowest after incubation for 60 min at pH 7.0 and 8.0 Gossypium hirsutum

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.7 heme
-
 Gossypium hirsutum