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Literature summary extracted from

  • Bhiri, F.; Chaabouni, S.E.; Limam, F.; Ghrir, R.; Marzouki, N.
    Purification and biochemical characterization of extracellular beta-glucosidases from the hypercellulolytic Pol6 mutant of Penicillium occitanis (2008), Appl. Biochem. Biotechnol., 149, 169-182.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.21 additional information isolation of isoforms beta-glu 1 and beta-glu 2 from hypercellulolytic Pol6 mutant Penicillium sp. 'occitanis'

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 Cu2+ 2 mM, significant inhibition, both isoforms beta-glu 2 and beta-glu 1 Penicillium sp. 'occitanis'
3.2.1.21 D-glucose competitive, both isoforms beta-glu 2 and beta-glu 1 Penicillium sp. 'occitanis'
3.2.1.21 delta-gluconolactone competitive, both isoforms beta-glu 2 and beta-glu 1 Penicillium sp. 'occitanis'
3.2.1.21 Hg2+ 2 mM, significant inhibition, both isoforms beta-glu 2 and beta-glu 1 Penicillium sp. 'occitanis'

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.37
-
 cellobiose both isoforms beta-glu 2 and beta-glu 1 Penicillium sp. 'occitanis'
3.2.1.21 1.43
-
 4-nitrophenyl beta-D-glucopyranoside both isoforms beta-glu 2 and beta-glu 1 Penicillium sp. 'occitanis'

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 98000
-
 x * 98000, SDS-PAGE, both isoforms beta-glu 1 and beta-glu 2 from hypercellulolytic Pol6 mutant Penicillium sp. 'occitanis'

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Penicillium sp. 'occitanis'
-
 isolation of isoforms beta-glu 1 and beta-glu 2 from hypercellulolytic Pol6 mutant
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.21 glycoprotein 8% carbohydrate content, both isoforms beta-glu 1 and beta-glu 2 from hypercellulolytic Pol6 mutant Penicillium sp. 'occitanis'

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 isolation of isoforms beta-glu 1 and beta-glu 2 from hypercellulolytic Pol6 mutant Penicillium sp. 'occitanis'

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 culture fluid
-
 Penicillium sp. 'occitanis'
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 103
-
 substrate 4-nitrophenyl beta-D-glucopyranoside, pH 4.8, 60°C, isoform beta-glu 2 Penicillium sp. 'occitanis'
3.2.1.21 173
-
 substrate 4-nitrophenyl beta-D-glucopyranoside, pH 4.8, 60°C, isoform beta-glu 2 Penicillium sp. 'occitanis'

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Penicillium sp. 'occitanis' 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Penicillium sp. 'occitanis' 2 beta-D-glucose
-
 ?
3.2.1.21 additional information in presence of high concentrations of glucose, both isoforms show trans-glycosidase activity Penicillium sp. 'occitanis' ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 ? x * 98000, SDS-PAGE, both isoforms beta-glu 1 and beta-glu 2 from hypercellulolytic Pol6 mutant Penicillium sp. 'occitanis'

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.21 Penicillium sp. 'occitanis' isoelectric focusing, isoform beta-glu 2
-
 5.3
3.2.1.21 Penicillium sp. 'occitanis' isoelectric focusing, isoform beta-glu 1
-
 5.5