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Literature summary extracted from
- A colorimetric assay for sulfiredoxin activity using inorganic phosphate measurement (2009), Anal. Biochem., 393, 36-40.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.8.98.2 | drug development | Srx may be a potential target for prevention or treatment of cancer, the colorimetric assay would be useful for high-throughput screening of Srx inhibitors as demonstrated | Homo sapiens |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.98.2 | into the vector pET14b for expression in Escherichia coli BL21 cells | Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.8.98.2 | C99S | mutant, substitution of serine for Cys99 blocks the release of phosphate | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.98.2 | additional information | screening for Srx inhibitors is carried out in an automated setup using 25000 chemicals | Homo sapiens |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.98.2 | Mg2+ | - |
Homo sapiens |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.98.2 | peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + R-SH | Homo sapiens | - |
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.98.2 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.98.2 | His-Srx proteins are purified from cell lysates by chromatography on a column on Ni-chelating Sepharose | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.98.2 | peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + R-SH | - |
Homo sapiens | peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.98.2 | Srx | - |
Homo sapiens |
| 1.8.98.2 | sulfiredoxin | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.98.2 | 23 | - |
malachite green assay at room temperature | Homo sapiens |
| 1.8.98.2 | 30 | - |
kinetic assay | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.98.2 | 7.5 | - |
- |
Homo sapiens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.98.2 | ATP | - |
Homo sapiens | |
| 1.8.98.2 | glutathione | - |
Homo sapiens | |
| 1.8.98.2 | thioredoxin | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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