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Literature summary extracted from

  • Seki, E.; Matsuda, N.; Yokoyama, S.; Kigawa, T.
    Cell-free protein synthesis system from Escherichia coli cells cultured at decreased temperatures improves productivity by decreasing DNA template degradation (2008), Anal. Biochem., 377, 156-161.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.11.5 additional information Escherichia coli RecD protein expression level is decreased at lower cultivation temperature, which greatly improves the productivity of cell-free protein synthesis from linear DNA templates ?
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 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.11.5 Escherichia coli
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 BL21 strain and BL21-RecD-SBP strain
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.11.5 double-stranded DNA + H2O BL21 and BL21-RecD-SBP strains share similar linear DNA degradation activities Escherichia coli ?
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 ?
3.1.11.5 additional information RecD protein expression level is decreased at lower cultivation temperature, which greatly improves the productivity of cell-free protein synthesis from linear DNA templates Escherichia coli ?
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 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.11.5 exonuclease V
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 Escherichia coli
3.1.11.5 RecD
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 Escherichia coli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.11.5 additional information
-
 by altering the cultivation temperature (37°C) of the cells to a moderately lower range (20-34°C), dramatically reduces the linear DNA degradation activity of RecD Escherichia coli