EC Number | Cloned (Comment) | Organism |
---|---|---|
1.4.5.1 | expression in Escherichia coli | Helicobacter pylori |
1.4.99.6 | expressed in Escherichia coli BL21(DE3) cells | Helicobacter pylori |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.5.1 | benzoate | 0.1 mM, 98% inhibition | Helicobacter pylori | |
1.4.5.1 | iodoacetamide | 0.1 mM, 66% inhibition | Helicobacter pylori | |
1.4.5.1 | iodoacetic acid | 0.1 mM, 62% inhibition | Helicobacter pylori | |
1.4.5.1 | additional information | EDTA, Mg2+ and Ca2+ have no effect on DadA activity | Helicobacter pylori | |
1.4.5.1 | p-hydroxybenzoic acid | 0.1 mM, 79% inhibition | Helicobacter pylori | |
1.4.99.6 | 4-hydroxybenzoic acid | 21.4% residual activity at 0.1 mM | Helicobacter pylori | |
1.4.99.6 | benzoate | 2% residual activity at 0.1 mM | Helicobacter pylori | |
1.4.99.6 | iodoacetamide | 34.2% residual activity at 0.1 mM | Helicobacter pylori | |
1.4.99.6 | iodoacetate | 38.2% residual activity at 0.1 mM | Helicobacter pylori | |
1.4.99.6 | additional information | not inhibited by EDTA | Helicobacter pylori |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.5.1 | 0.0082 | - |
oxidized coenzyme Q1 | pH 8.0, 37°C | Helicobacter pylori | |
1.4.5.1 | 40.2 | - |
D-proline | pH 8.0, 37°C | Helicobacter pylori | |
1.4.99.6 | 0.0082 | - |
coenzyme Q1 | in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C | Helicobacter pylori | |
1.4.99.6 | 40.2 | - |
D-Pro | with Q1 as the electron acceptor, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C | Helicobacter pylori |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.4.5.1 | additional information | Mg2+ and Ca2+ have no effect on DadA activity | Helicobacter pylori | |
1.4.99.6 | additional information | not influenced by Mg2+ and Ca+ | Helicobacter pylori |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.5.1 | 44000 | - |
x * 44000, SDS-PAGE | Helicobacter pylori |
1.4.5.1 | 46040 | - |
x * 46040, calculated from sequence | Helicobacter pylori |
1.4.99.6 | 44000 | - |
x * 44000, SDS-PAGE | Helicobacter pylori |
1.4.99.6 | 46040 | - |
x * 46040, calculated from amino acid sequence | Helicobacter pylori |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.99.6 | D-Pro + H2O + coenzyme Q1 | Helicobacter pylori | D-Pro is the best substrate | ? | - |
? | |
1.4.99.6 | D-Pro + H2O + coenzyme Q1 | Helicobacter pylori NCTC 11637 | D-Pro is the best substrate | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.5.1 | Helicobacter pylori | A3KEZ1 | NCTC 11637 | - |
1.4.99.6 | Helicobacter pylori | A3KEZ1 | - |
- |
1.4.99.6 | Helicobacter pylori NCTC 11637 | A3KEZ1 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.5.1 | from Helicobacter pylori and recombinant Escherichia coli cells | Helicobacter pylori |
1.4.99.6 | DEAE-Toyopearl column chromatography | Helicobacter pylori |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.4.5.1 | 6.22 | - |
- |
Helicobacter pylori |
1.4.99.6 | 0.05 | - |
purified enzyme, with 20 mM D-Asp as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C | Helicobacter pylori |
1.4.99.6 | 0.05 | - |
purified enzyme, with 20 mM D-Met as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C | Helicobacter pylori |
1.4.99.6 | 0.05 | - |
purified enzyme, with 20 mM D-Val as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C | Helicobacter pylori |
1.4.99.6 | 0.06 | - |
purified enzyme, with 20 mM D-Glu as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C | Helicobacter pylori |
1.4.99.6 | 0.12 | - |
purified enzyme, with 20 mM L-Ala as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C | Helicobacter pylori |
1.4.99.6 | 0.22 | - |
purified enzyme, with 20 mM L-Pro as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C | Helicobacter pylori |
1.4.99.6 | 0.46 | - |
cell-free extract, with 20 mM D-Pro as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C | Helicobacter pylori |
1.4.99.6 | 1.25 | - |
purified enzyme, with 20 mM D-Ser as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C | Helicobacter pylori |
1.4.99.6 | 2.29 | - |
purified enzyme, with 20 mM D-Ala as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C | Helicobacter pylori |
1.4.99.6 | 6.22 | - |
purified enzyme, with 20 mM D-Pro as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C | Helicobacter pylori |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.5.1 | D-Ala + oxidized coenzyme Q1 + H2O | specific activity is 37% of that with D-proline | Helicobacter pylori | pyruvate + NH3 + reduced coenzyme Q1 | - |
? | |
1.4.5.1 | D-Phe + oxidized coenzyme Q1 + H2O | specific activity is 20% of that with D-proline | Helicobacter pylori | phenylpyruvate + NH3 + reduced coenzyme Q1 | - |
? | |
1.4.5.1 | D-proline + oxidized coenzyme Q1 + H2O | - |
Helicobacter pylori | DELTA1-pyrroline-2-carboxylate + NH3 + reduced coenzyme Q1 | - |
? | |
1.4.5.1 | D-Ser + oxidized coenzyme Q1 + H2O | specific activity is 7% of that with D-proline | Helicobacter pylori | 3-hydroxy-2-oxopropanoate + NH3 + reduced coenzyme Q1 | - |
? | |
1.4.5.1 | additional information | activity with D-Val, D-Met, D-Glu, D-Asp, L-Pro and L-Ala is below 5% of the activity with D-Pro | Helicobacter pylori | ? | - |
? | |
1.4.99.6 | D-Ala + H2O + oxidized 2,6-dichlorophenolindophenol | 36.8% activity compared to D-Pro | Helicobacter pylori | pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.4.99.6 | D-Ala + H2O + oxidized 2,6-dichlorophenolindophenol | 36.8% activity compared to D-Pro | Helicobacter pylori NCTC 11637 | pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.4.99.6 | D-Asp + H2O + oxidized 2,6-dichlorophenolindophenol | 0.8% activity compared to D-Pro | Helicobacter pylori | 2-oxobutanedioate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.4.99.6 | D-Asp + H2O + oxidized 2,6-dichlorophenolindophenol | 0.8% activity compared to D-Pro | Helicobacter pylori NCTC 11637 | 2-oxobutanedioate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.4.99.6 | D-Glu + H2O + oxidized 2,6-dichlorophenolindophenol | 1.0% activity compared to D-Pro | Helicobacter pylori | 2-oxoglutarate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.4.99.6 | D-Glu + H2O + oxidized 2,6-dichlorophenolindophenol | 1.0% activity compared to D-Pro | Helicobacter pylori NCTC 11637 | 2-oxoglutarate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.4.99.6 | D-Met + H2O + oxidized 2,6-dichlorophenolindophenol | 0.8% activity compared to D-Pro | Helicobacter pylori | 4-methylsulfanyl-2-oxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.4.99.6 | D-Met + H2O + oxidized 2,6-dichlorophenolindophenol | 0.8% activity compared to D-Pro | Helicobacter pylori NCTC 11637 | 4-methylsulfanyl-2-oxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.4.99.6 | D-Phe + H2O + oxidized 2,6-dichlorophenolindophenol | 19.8% activity compared to D-Pro | Helicobacter pylori | phenylpyruvate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.4.99.6 | D-Pro + H2O + coenzyme Q1 | D-Pro is the best substrate | Helicobacter pylori | ? | - |
? | |
1.4.99.6 | D-Pro + H2O + coenzyme Q1 | D-Pro is the best substrate | Helicobacter pylori NCTC 11637 | ? | - |
? | |
1.4.99.6 | D-Pro + H2O + oxidized 2,6-dichlorophenolindophenol | 100% activity | Helicobacter pylori | DELTA1-pyrroline-2-carboxylate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.4.99.6 | D-Ser + H2O + oxidized 2,6-dichlorophenolindophenol | 6.7% activity compared to D-Pro | Helicobacter pylori | 3-hydroxy-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.4.99.6 | D-Val + H2O + oxidized 2,6-dichlorophenolindophenol | 0.8% activity compared to D-Pro | Helicobacter pylori | 2-oxoisopentanoate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.4.99.6 | L-Ala + H2O + oxidized 2,6-dichlorophenolindophenol | 1.9% activity compared to D-Pro | Helicobacter pylori | ? | - |
? | |
1.4.99.6 | L-Pro + H2O + oxidized 2,6-dichlorophenolindophenol | 3.5% activity compared to D-Pro | Helicobacter pylori | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.5.1 | ? | x * 44000, SDS-PAGE | Helicobacter pylori |
1.4.5.1 | ? | x * 46040, calculated from sequence | Helicobacter pylori |
1.4.99.6 | ? | x * 44000, SDS-PAGE | Helicobacter pylori |
1.4.99.6 | ? | x * 46040, calculated from amino acid sequence | Helicobacter pylori |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.5.1 | DadA | - |
Helicobacter pylori |
1.4.99.6 | D-amino acid dehydrogenase | - |
Helicobacter pylori |
1.4.99.6 | DAD | - |
Helicobacter pylori |
1.4.99.6 | DadA | - |
Helicobacter pylori |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.5.1 | 37 | - |
- |
Helicobacter pylori |
1.4.99.6 | 37 | - |
- |
Helicobacter pylori |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.5.1 | 25 | 55 | 25°C: about 50% of maximal activity, 55°C: about 65% of maximal activity | Helicobacter pylori |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.5.1 | 8 | - |
- |
Helicobacter pylori |
1.4.99.6 | 8 | - |
- |
Helicobacter pylori |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.4.5.1 | 7.5 | 8.5 | pH 7.5: about 50% of maximal activity, pH 8.5: about 45% of maximal activity | Helicobacter pylori |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.5.1 | FAD | flavoenzyme | Helicobacter pylori | |
1.4.99.6 | FAD | - |
Helicobacter pylori |