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Literature summary extracted from

  • Hatzopoulos, G.N.; Kefala, G.; Mueller-Dieckmann, J.
    Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of isocitrate dehydrogenase 2 (Rv0066c) from Mycobacterium tuberculosis (2008), Acta Crystallogr. Sect. F, 64, 1139-1142.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.41 expressed in Escherichia coli BL21(DE3)RP cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.41 sitting drop vapour diffusion method, using 3-5% (w/v) PEG 20000, 0.1 M MES pH 5.7 and 0.2 M CaCl2 Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.41 83181
-
 3 * 83181, calculated from amino acid sequence Mycobacterium tuberculosis
1.1.1.41 250000
-
 Superose 6 gel filtration Mycobacterium tuberculosis
1.1.1.41 260000
-
 dynamic light scattering Mycobacterium tuberculosis
1.1.1.41 290000
-
 Superdex 200 gel filtration Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.41 Mycobacterium tuberculosis
-
-
-
1.1.1.41 Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.41 Hi-Trap chelating column chromatography and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.41 D-isocitrate + NAD+
-
 Mycobacterium tuberculosis 2-oxoglutarate + CO2 + NADH
-
 ?
1.1.1.41 D-isocitrate + NAD+
-
 Mycobacterium tuberculosis H37Rv 2-oxoglutarate + CO2 + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.41 trimer 3 * 83181, calculated from amino acid sequence Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.41 Icd-2
-
 Mycobacterium tuberculosis
1.1.1.41 isocitrate dehydrogenase 2
-
 Mycobacterium tuberculosis
1.1.1.41 Rv0066c
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.41 NAD+
-
 Mycobacterium tuberculosis