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Literature summary extracted from
- Structure of Hordeum vulgare NADPH-dependent thioredoxin reductase 2. Unwinding the reaction mechanism (2009), Acta Crystallogr. Sect. D, 65, 932-941.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.1.9 | expressed in Escherichia coli Rosetta (DE3) cells | Hordeum vulgare |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.8.1.9 | hanging drop vapor diffusion method, using 24% (w/v) PEG 400, 2% Jeffamine M-600, and 0.1 M citrate buffer pH 3.5 | Hordeum vulgare |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.9 | Hordeum vulgare | A9LN30 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.1.9 | HisTrap HP affinity column chromatography, gel filtration | Hordeum vulgare |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.9 | thioredoxin disulfide + NADPH + H+ | - |
Hordeum vulgare | thioredoxin + NADP+ | - |
? |  |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.1.9 | homodimer | x-ray crystallography | Hordeum vulgare |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.1.9 | NADPH-dependent thioredoxin reductase 2 | - |
Hordeum vulgare |
| 1.8.1.9 | NTR2 | - |
Hordeum vulgare |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.9 | FAD | contains one FAD molecule per subunit | Hordeum vulgare | |
| 1.8.1.9 | NADPH | dependent on | Hordeum vulgare | |
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Release 2023.1 (January 2023)
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