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Literature summary extracted from

  • Javid-Majd, F.; Yang, D.; Ioerger, T.R.; Sacchettini, J.C.
    The 1.25 A resolution structure of phosphoribosyl-ATP pyrophosphohydrolase from Mycobacterium tuberculosis (2008), Acta Crystallogr. Sect. D, 64, 627-635.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.6.1.31 drug development because of its essentiality for growth in vitro, HisE is a potential drug target for tuberculosis Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.31 native and selenomethionine-labeled, uncomplexed enzyme in two crystal forms, hanging-drop vapor diffusion method, 10 mg/ml protein in 20 mM HEPES, pH 7.5, mixing of protein solution and reservoir buffer containing 100 mM sodium cacodylate pH 6.5, 1.0 M sodium citrate, 0.1 M guanidinium hydrochloride in a 1:1 ratio, 4 days, X-ray structure determination and analysis at 1.25 A and 1.79 A resolution, respectively Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.31 Mg2+
-
 Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.31 1-(5-phosphoribosyl)-ATP + H2O Mycobacterium tuberculosis phosphoribosyl-ATP pyrophosphohydrolase is the second enzyme in the histidine-biosynthetic pathway, irreversibly hydrolyzing phosphoribosyl-ATP to phosphoribosyl-AMP and pyrophosphate 1-(5-phosphoribosyl)-AMP + diphosphate
-
 ir
3.6.1.31 1-(5-phosphoribosyl)-ATP + H2O Mycobacterium tuberculosis H37Rv phosphoribosyl-ATP pyrophosphohydrolase is the second enzyme in the histidine-biosynthetic pathway, irreversibly hydrolyzing phosphoribosyl-ATP to phosphoribosyl-AMP and pyrophosphate 1-(5-phosphoribosyl)-AMP + diphosphate
-
 ir
3.6.1.31 additional information Mycobacterium tuberculosis HisE essentiality for growth in vitro ?
-
 ?
3.6.1.31 additional information Mycobacterium tuberculosis H37Rv HisE essentiality for growth in vitro ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.31 Mycobacterium tuberculosis P9WMM9 gene hisE
-
3.6.1.31 Mycobacterium tuberculosis H37Rv P9WMM9 gene hisE
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.31 1-(5-phosphoribosyl)-ATP + H2O active site structure, overview Mycobacterium tuberculosis 1-(5-phosphoribosyl)-AMP + diphosphate
-
 ir
3.6.1.31 1-(5-phosphoribosyl)-ATP + H2O phosphoribosyl-ATP pyrophosphohydrolase is the second enzyme in the histidine-biosynthetic pathway, irreversibly hydrolyzing phosphoribosyl-ATP to phosphoribosyl-AMP and pyrophosphate Mycobacterium tuberculosis 1-(5-phosphoribosyl)-AMP + diphosphate
-
 ir
3.6.1.31 1-(5-phosphoribosyl)-ATP + H2O active site structure, overview Mycobacterium tuberculosis H37Rv 1-(5-phosphoribosyl)-AMP + diphosphate
-
 ir
3.6.1.31 1-(5-phosphoribosyl)-ATP + H2O phosphoribosyl-ATP pyrophosphohydrolase is the second enzyme in the histidine-biosynthetic pathway, irreversibly hydrolyzing phosphoribosyl-ATP to phosphoribosyl-AMP and pyrophosphate Mycobacterium tuberculosis H37Rv 1-(5-phosphoribosyl)-AMP + diphosphate
-
 ir
3.6.1.31 additional information HisE essentiality for growth in vitro Mycobacterium tuberculosis ?
-
 ?
3.6.1.31 additional information HisE essentiality for growth in vitro Mycobacterium tuberculosis H37Rv ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.31 homodimer the biological unit of the protein is a homodimer, with an active site on each subunit composed of residues exclusively from that subunit, the protein is composed of five alpha-helices with connecting loops Mycobacterium tuberculosis
3.6.1.31 More structure comparisons, overview Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
3.6.1.31 HisE
-
 Mycobacterium tuberculosis
3.6.1.31 More the enzyme is a member of the alpha-helical nucleoside-triphosphate pyrophosphatase superfamily Mycobacterium tuberculosis
3.6.1.31 phosphoribosyl-ATP pyrophosphohydrolase
-
 Mycobacterium tuberculosis