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Literature summary extracted from
- Application of maximum-entropy maps in the accurate refinement of a putative acylphosphatase using 1.3 A X-ray diffraction data (2008), Acta Crystallogr. Sect. D, 64, 237-247.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.6.1.7 | expression of TT0497 in Escherichia coli strain BL21(DE3) | Thermus thermophilus |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.6.1.7 | purified recombinant TT0497, 0.0005 ml of 2.9 mg/ml protein in 20 mM Tris-HCl pH 8.0, 0.2 M NaCl is mixed with 0.0005 ml of precipitant solution containing 27.5% w/v PEG 4000 and 0.1 M MES, pH 6.9, X-ray diffraction structure determination and analysis at 1.3 A resolution, structural refinement based on the Fourier method, modelling, overview | Thermus thermophilus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.6.1.7 | Thermus thermophilus | Q5SKS6 | - |
- |
| 3.6.1.7 | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | Q5SKS6 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.6.1.7 | recombinant TT0497 from Escherichia coli strain BL21(DE3) by two steps of ion exchange chromatography, and gel filtration | Thermus thermophilus |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.6.1.7 | More | structure modelling, multiple conformations of the side chains of amino-acid residues | Thermus thermophilus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.6.1.7 | TT0497 | - |
Thermus thermophilus |
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Release 2023.1 (January 2023)
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