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Literature summary extracted from

  • Szurmak, B.; Wyslouch-Cieszyeska, A.; Wszelaka-Rylik, M.; Bal, W.; Dobrzanska, M.
    A diadenosine 5',5''-P1P4 tetraphosphate (Ap4A) hydrolase from Arabidopsis thaliana that is activated preferentially by Mn2+ ions (2008), Acta Biochim. Pol., 55, 151-160.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.17 overexpression in Escherichia coli Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.17 Ca2+ no activity in the presence of 1.0 and 5.0 mM Ca2+ Arabidopsis thaliana
3.6.1.17 Cd2+ no activity in the presence of 1.0 mM Cd2+, weak enzyme activation at 5 mM (4.% compared to 1.5 mM Mn2+) Arabidopsis thaliana
3.6.1.17 Mg2+ no activity in the presence of 1 mM Mg2+, 9.1% relative activity at 5 mM Mg2+ compared to 1.5 mM Mn2+ Arabidopsis thaliana
3.6.1.17 Mn2+ enzyme preferentially hydrolyses diadenosine 5',5'-P1P4 tetraphosphate in the form of a Mn2+ complex at a concentration of 1.5 mM, 37.7% relative activity at 0.5 mM compared to 1.5 mM Mn2+ Arabidopsis thaliana
3.6.1.17 Zn2+ enzyme exhibit residual activity in the presence of 1.5 mM Zn2+ ions, 28.4% relative activity at 5 mM Zn2+ compared to 1.5 mM Mn2+ Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.17 21670
-
by electrospray ionization quadrupole time-of-flight mass spectrometry Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.17 diadenosine 5',5'-P1,P4-tetraphosphate + H2O Arabidopsis thaliana key enzyme controlling the in vivo concentration of diadenosine 5',5'-P1,P4 tetraphosphate (Ap4A) - an important signaling molecule involved in regulation of DNA replication and repair, signaling in stress response and apoptosis ATP + AMP + ?
-
?
3.6.1.17 diadenosine 5',5'-P1,P5-pentaphosphate + H2O Arabidopsis thaliana
-
ATP + ADP
-
?
3.6.1.17 diguanosine 5',5'-P1,P4-tetraphosphate + H2O Arabidopsis thaliana
-
GTP + GMP
-
?
3.6.1.17 additional information Arabidopsis thaliana enzyme hydrolyses bis(5'-nucleosidyl) polyphosphates (Np4-6N') in such a way that a nucleosidyl triphosphate is always one of the products ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.17 Arabidopsis thaliana Q9C6Z2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.17 by affinity chromatography by means of nickel-nitrilotriacetic acid agarose Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.17 5.9
-
Mn2+ concentration 0.15 mM Arabidopsis thaliana
3.6.1.17 19.2
-
Mn2+ concentration 0.75 mM Arabidopsis thaliana
3.6.1.17 27
-
maximal activity of the enzyme coincides with total Mn2+ and Ap4A concentrations of 1 mM, at such concentrations the Mn(Ap4A) complex dominates and the concentration of its components Mn2+ = Ap4A are 0.083 mM, an excess of either Mn2+ or Ap4A over Mn(Ap4A) results in a decrease of the reaction rate Arabidopsis thaliana
3.6.1.17 31.1
-
Mn2+ concentration 1.5 mM Arabidopsis thaliana
3.6.1.17 55.2
-
Mn2+ concentration 3.5 mM Arabidopsis thaliana
3.6.1.17 70.5
-
Mn2+ concentration 6.5 mM Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.17 diadenosine 5',5'-P1,P4-tetraphosphate + H2O key enzyme controlling the in vivo concentration of diadenosine 5',5'-P1,P4 tetraphosphate (Ap4A) - an important signaling molecule involved in regulation of DNA replication and repair, signaling in stress response and apoptosis Arabidopsis thaliana ATP + AMP + ?
-
?
3.6.1.17 diadenosine 5',5'-P1,P5-pentaphosphate + H2O
-
Arabidopsis thaliana ATP + ADP
-
?
3.6.1.17 diguanosine 5',5'-P1,P4-tetraphosphate + H2O
-
Arabidopsis thaliana GTP + GMP
-
?
3.6.1.17 additional information enzyme hydrolyses bis(5'-nucleosidyl) polyphosphates (Np4-6N') in such a way that a nucleosidyl triphosphate is always one of the products Arabidopsis thaliana ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.17 Ap4A hydrolase
-
Arabidopsis thaliana
3.6.1.17 asymmetrical bis-(5'-nucleosidyl)-tetraphosphatase
-
Arabidopsis thaliana
3.6.1.17 AtNUDX25 protein
-
Arabidopsis thaliana
3.6.1.17 diadenosine 5',5'-P1P4 tetraphosphate hydrolase
-
Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.17 8
-
assay at Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.17 6.8 9.5 in the presence of 3.5 mM Mn2+, the enzyme is active over a wide pH range Arabidopsis thaliana

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.6.1.17 Arabidopsis thaliana deduced pI from amino acid sequence
-
4.1