EC Number | Cloned (Comment) | Organism |
---|---|---|
3.6.1.17 | overexpression in Escherichia coli | Arabidopsis thaliana |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.17 | Ca2+ | no activity in the presence of 1.0 and 5.0 mM Ca2+ | Arabidopsis thaliana | |
3.6.1.17 | Cd2+ | no activity in the presence of 1.0 mM Cd2+, weak enzyme activation at 5 mM (4.% compared to 1.5 mM Mn2+) | Arabidopsis thaliana | |
3.6.1.17 | Mg2+ | no activity in the presence of 1 mM Mg2+, 9.1% relative activity at 5 mM Mg2+ compared to 1.5 mM Mn2+ | Arabidopsis thaliana | |
3.6.1.17 | Mn2+ | enzyme preferentially hydrolyses diadenosine 5',5'-P1P4 tetraphosphate in the form of a Mn2+ complex at a concentration of 1.5 mM, 37.7% relative activity at 0.5 mM compared to 1.5 mM Mn2+ | Arabidopsis thaliana | |
3.6.1.17 | Zn2+ | enzyme exhibit residual activity in the presence of 1.5 mM Zn2+ ions, 28.4% relative activity at 5 mM Zn2+ compared to 1.5 mM Mn2+ | Arabidopsis thaliana |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.6.1.17 | 21670 | - |
by electrospray ionization quadrupole time-of-flight mass spectrometry | Arabidopsis thaliana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.17 | diadenosine 5',5'-P1,P4-tetraphosphate + H2O | Arabidopsis thaliana | key enzyme controlling the in vivo concentration of diadenosine 5',5'-P1,P4 tetraphosphate (Ap4A) - an important signaling molecule involved in regulation of DNA replication and repair, signaling in stress response and apoptosis | ATP + AMP + ? | - |
? | |
3.6.1.17 | diadenosine 5',5'-P1,P5-pentaphosphate + H2O | Arabidopsis thaliana | - |
ATP + ADP | - |
? | |
3.6.1.17 | diguanosine 5',5'-P1,P4-tetraphosphate + H2O | Arabidopsis thaliana | - |
GTP + GMP | - |
? | |
3.6.1.17 | additional information | Arabidopsis thaliana | enzyme hydrolyses bis(5'-nucleosidyl) polyphosphates (Np4-6N') in such a way that a nucleosidyl triphosphate is always one of the products | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.17 | Arabidopsis thaliana | Q9C6Z2 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.6.1.17 | by affinity chromatography by means of nickel-nitrilotriacetic acid agarose | Arabidopsis thaliana |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.6.1.17 | 5.9 | - |
Mn2+ concentration 0.15 mM | Arabidopsis thaliana |
3.6.1.17 | 19.2 | - |
Mn2+ concentration 0.75 mM | Arabidopsis thaliana |
3.6.1.17 | 27 | - |
maximal activity of the enzyme coincides with total Mn2+ and Ap4A concentrations of 1 mM, at such concentrations the Mn(Ap4A) complex dominates and the concentration of its components Mn2+ = Ap4A are 0.083 mM, an excess of either Mn2+ or Ap4A over Mn(Ap4A) results in a decrease of the reaction rate | Arabidopsis thaliana |
3.6.1.17 | 31.1 | - |
Mn2+ concentration 1.5 mM | Arabidopsis thaliana |
3.6.1.17 | 55.2 | - |
Mn2+ concentration 3.5 mM | Arabidopsis thaliana |
3.6.1.17 | 70.5 | - |
Mn2+ concentration 6.5 mM | Arabidopsis thaliana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.17 | diadenosine 5',5'-P1,P4-tetraphosphate + H2O | key enzyme controlling the in vivo concentration of diadenosine 5',5'-P1,P4 tetraphosphate (Ap4A) - an important signaling molecule involved in regulation of DNA replication and repair, signaling in stress response and apoptosis | Arabidopsis thaliana | ATP + AMP + ? | - |
? | |
3.6.1.17 | diadenosine 5',5'-P1,P5-pentaphosphate + H2O | - |
Arabidopsis thaliana | ATP + ADP | - |
? | |
3.6.1.17 | diguanosine 5',5'-P1,P4-tetraphosphate + H2O | - |
Arabidopsis thaliana | GTP + GMP | - |
? | |
3.6.1.17 | additional information | enzyme hydrolyses bis(5'-nucleosidyl) polyphosphates (Np4-6N') in such a way that a nucleosidyl triphosphate is always one of the products | Arabidopsis thaliana | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.1.17 | Ap4A hydrolase | - |
Arabidopsis thaliana |
3.6.1.17 | asymmetrical bis-(5'-nucleosidyl)-tetraphosphatase | - |
Arabidopsis thaliana |
3.6.1.17 | AtNUDX25 protein | - |
Arabidopsis thaliana |
3.6.1.17 | diadenosine 5',5'-P1P4 tetraphosphate hydrolase | - |
Arabidopsis thaliana |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.17 | 8 | - |
assay at | Arabidopsis thaliana |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.17 | 6.8 | 9.5 | in the presence of 3.5 mM Mn2+, the enzyme is active over a wide pH range | Arabidopsis thaliana |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.6.1.17 | Arabidopsis thaliana | deduced pI from amino acid sequence | - |
4.1 |