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Literature summary extracted from

  • Jin, Y.; Lee, W.H.; Zeng, L.; Zhang, Y.
    Molecular characterization of L-amino acid oxidase from king cobra venom (2007), Toxicon, 50, 479-489.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.4.3.2 medicine LAAO does not induce platelet aggregation, but it has potent inhibitory activity on platelet aggregation induced by ADP and U46619. It shows no effect on platelet aggregation induced by thrombin, mucetin, ristocetin and stejnulxin Ophiophagus hannah

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.2
-
Bungarus fasciatus
1.4.3.2
-
Bungarus multicinctus
1.4.3.2
-
Naja atra
1.4.3.2 into pGEM-T vector Ophiophagus hannah

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.2 0.7
-
L-methionine
-
Ophiophagus hannah
1.4.3.2 2.2
-
L-leucine
-
Ophiophagus hannah
1.4.3.2 2.3
-
L-histidine
-
Ophiophagus hannah
1.4.3.2 2.6
-
L-lysine
-
Ophiophagus hannah
1.4.3.2 2.9
-
L-isoleucine
-
Ophiophagus hannah

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.2 53686
-
1 * 64000, SDS-PAGE under non-reducing conditions, 1 * 67000, SDS-PAGE under reducing conditions, 1 * 53686, sequence analysis Ophiophagus hannah
1.4.3.2 64000
-
1 * 64000, SDS-PAGE under non-reducing conditions, 1 * 67000, SDS-PAGE under reducing conditions, 1 * 53686, sequence analysis Ophiophagus hannah
1.4.3.2 67000
-
1 * 64000, SDS-PAGE under non-reducing conditions, 1 * 67000, SDS-PAGE under reducing conditions, 1 * 53686, sequence analysis Ophiophagus hannah

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.2 Bungarus fasciatus A8QL52
-
-
1.4.3.2 Bungarus multicinctus A8QL51
-
-
1.4.3.2 Naja atra A8QL58
-
-
1.4.3.2 Ophiophagus hannah P81383
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.2 by gel filtration, ion-exchange and heparin chromatography, to homogeneity Ophiophagus hannah

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.2 venom
-
Ophiophagus hannah
-
1.4.3.2 venom
-
Bungarus fasciatus
-
1.4.3.2 venom
-
Bungarus multicinctus
-
1.4.3.2 venom
-
Naja atra
-
1.4.3.2 venom gland
-
Ophiophagus hannah
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.2 L-histidine + H2O + O2
-
Ophiophagus hannah 3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
-
?
1.4.3.2 L-isoleucine + H2O + O2
-
Ophiophagus hannah 3-methyl-2-oxopentanoic acid + NH3 + H2O2
-
?
1.4.3.2 L-leucine + H2O + O2
-
Ophiophagus hannah 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
?
1.4.3.2 L-lysine + H2O + O2
-
Ophiophagus hannah 6-amino-2-oxohexanoic acid + NH3 + H2O2
-
?
1.4.3.2 L-methionine + H2O + O2
-
Ophiophagus hannah 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
?

Subunits

EC Number Subunits Comment Organism
1.4.3.2 monomer 1 * 64000, SDS-PAGE under non-reducing conditions, 1 * 67000, SDS-PAGE under reducing conditions, 1 * 53686, sequence analysis Ophiophagus hannah

Synonyms

EC Number Synonyms Comment Organism
1.4.3.2 LAAO
-
Ophiophagus hannah
1.4.3.2 LAAO
-
Bungarus fasciatus
1.4.3.2 LAAO
-
Bungarus multicinctus
1.4.3.2 LAAO
-
Naja atra