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Literature summary extracted from

  • Wei, X.L.; Wei, J.F.; Li, T.; Qiao, L.Y.; Liu, Y.L.; Huang, T.; He, S.H.
    Purification, characterization and potent lung lesion activity of an L-amino acid oxidase from Agkistrodon blomhoffii ussurensis snake venom (2007), Toxicon, 50, 1126-1139.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.4.3.2 medicine LAO potently induces lesions in lungs and livers. LAO causes severe pneumorrhagia, pulmonary interstitial edema, fusion of pulmonary alveoli, cardiac interstitial edema and bleeding when being intravenously injected into BALB/c mice. Also induces liver cell necrosis and release of cytokines including IL-6, IL-12 and IL-2 from highly purified human peripheral blood monocytes and T cells, respectively. The ability of LAO will contribute to the understanding of the pathogenesis of snakebite wound Gloydius ussuriensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.2 0.11
-
 L-leucine
-
 Gloydius ussuriensis
1.4.3.2 0.27
-
 L-valine
-
 Gloydius ussuriensis
1.4.3.2 0.36
-
 L-asparagine
-
 Gloydius ussuriensis
1.4.3.2 0.88
-
 L-methionine
-
 Gloydius ussuriensis
1.4.3.2 0.97
-
 L-lysine
-
 Gloydius ussuriensis
1.4.3.2 1.57
-
 L-tyrosine
-
 Gloydius ussuriensis
1.4.3.2 2.3
-
 L-tryptophan
-
 Gloydius ussuriensis
1.4.3.2 2.33
-
 L-arginine
-
 Gloydius ussuriensis
1.4.3.2 4.02
-
 L-isoleucine
-
 Gloydius ussuriensis
1.4.3.2 4.28
-
 L-phenylalanine
-
 Gloydius ussuriensis
1.4.3.2 4.97
-
 L-histidine
-
 Gloydius ussuriensis
1.4.3.2 5.12
-
 L-alanine
-
 Gloydius ussuriensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.2 58000
-
 2 * 60000, SDS-PAGE under reducing conditions, 2 * 58000, SDS-PAGE under nonreducing conditions Gloydius ussuriensis
1.4.3.2 60000
-
 2 * 60000, SDS-PAGE under reducing conditions, 2 * 58000, SDS-PAGE under nonreducing conditions Gloydius ussuriensis
1.4.3.2 108800
-
 gel filtration Gloydius ussuriensis

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.2 Gloydius ussuriensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.2 by gel filtration followed by ion-exchange chromatography, 51.3fold Gloydius ussuriensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.2 venom
-
 Gloydius ussuriensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.2 1.56
-
 crude venom Gloydius ussuriensis
1.4.3.2 79.92
-
 51.3fold purified Gloydius ussuriensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.2 L-alanine + H2O + O2
-
 Gloydius ussuriensis 2-oxopropanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-arginine + H2O + O2
-
 Gloydius ussuriensis 2-oxo-5-guanidinovaleric acid + NH3 + H2O2
-
 ?
1.4.3.2 L-asparagine + H2O + O2
-
 Gloydius ussuriensis alpha-ketosuccinamic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-histidine + H2O + O2
-
 Gloydius ussuriensis 3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-isoleucine + H2O + O2
-
 Gloydius ussuriensis 3-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-leucine + H2O + O2
-
 Gloydius ussuriensis 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-lysine + H2O + O2
-
 Gloydius ussuriensis 6-amino-2-oxohexanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-methionine + H2O + O2
-
 Gloydius ussuriensis 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-phenylalanine + H2O + O2
-
 Gloydius ussuriensis phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.2 L-tryptophan + H2O + O2
-
 Gloydius ussuriensis 3-indole-2-oxopropanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-tyrosine + H2O + O2
-
 Gloydius ussuriensis p-hydroxyphenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.2 L-valine + H2O + O2
-
 Gloydius ussuriensis 2-oxoisovalerate + NH3 + H2O2
-
 ?
1.4.3.2 additional information does not hydrolyze casein and synthetic substrates including N-benzoyl-L-arginine ethylester or N-benzoyl-L-arginine p-nitroanilide Gloydius ussuriensis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.2 dimer 2 * 60000, SDS-PAGE under reducing conditions, 2 * 58000, SDS-PAGE under nonreducing conditions Gloydius ussuriensis

Synonyms

EC Number Synonyms Comment Organism
1.4.3.2 LAO
-
 Gloydius ussuriensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.2 4.15
-
 L-histidine
-
 Gloydius ussuriensis
1.4.3.2 5.08
-
 L-valine
-
 Gloydius ussuriensis
1.4.3.2 5.26
-
 L-asparagine
-
 Gloydius ussuriensis
1.4.3.2 6.33
-
 L-isoleucine
-
 Gloydius ussuriensis
1.4.3.2 6.58
-
 L-tryptophan
-
 Gloydius ussuriensis
1.4.3.2 8.42
-
 L-tyrosine
-
 Gloydius ussuriensis
1.4.3.2 10.51
-
 L-lysine
-
 Gloydius ussuriensis
1.4.3.2 22.54
-
 L-arginine
-
 Gloydius ussuriensis
1.4.3.2 24.13
-
 L-methionine
-
 Gloydius ussuriensis
1.4.3.2 26.89
-
 L-alanine
-
 Gloydius ussuriensis
1.4.3.2 48.22
-
 L-leucine
-
 Gloydius ussuriensis
1.4.3.2 48.23
-
 L-phenylalanine
-
 Gloydius ussuriensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.2 5.2
-
-
 Gloydius ussuriensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.3.2 4.4 9.6
-
 Gloydius ussuriensis