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Literature summary extracted from

  • Canova, M.J.; Veyron-Churlet, R.; Zanella-Cleon, I.; Cohen-Gonsaud, M.; Cozzone, A.J.; Becchi, M.; Kremer, L.; Molle, V.
    The Mycobacterium tuberculosis serine/threonine kinase PknL phosphorylates Rv2175c: mass spectrometric profiling of the activation loop phosphorylation sites and their role in the recruitment of Rv2175c (2008), Proteomics, 8, 521-533.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.1 the cytosolic domain of PknL (DELTA370-399) is expressed in Escherichia coli as a GST-fusion protein Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.1 DELTA370-399/K48M mutant protein shows no autophosphorylation activity, indicating that Lys48 is essential for catalysing the phosphorylation reaction Mycobacterium tuberculosis
2.7.11.1 DELTA370-399/S171A mutant protein shows similar autophosphorylation activity compared to wild-type, indicating that Ser171 is not an autophosphorylated residue Mycobacterium tuberculosis
2.7.11.1 DELTA370-399/S174A mutant protein shows similar autophosphorylation activity compared to wild-type, indicating that Ser171 is not an autophosphorylated residue Mycobacterium tuberculosis
2.7.11.1 DELTA370-399/T173A mutant protein shows an autophosphorylation activity of only 19% compared to wild-type Mycobacterium tuberculosis
2.7.11.1 DELTA370-399/T173A/T175A mutant protein shows an autophosphorylation activity of only 19% compared to wild-type Mycobacterium tuberculosis
2.7.11.1 DELTA370-399/T175A mutant protein shows an autophosphorylation activity of only 46% compared to wild-type Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.1 43000
-
 deduced from cDNA Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.1 Mycobacterium tuberculosis P9WI63
-
-
2.7.11.1 Mycobacterium tuberculosis H37Rv P9WI63
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.1 phosphoprotein enzyme possesses autophosphorylation activity. The activation loop phosphorylated residues Thr173 and Thr175 are essential for the autophosphorylation activity of PknL. Phosphorylation of the activation loop Thr173 residue is also required for optimal PknL-mediated phosphorylation of Rv2175c Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.1 ATP + Rv2175
-
 Mycobacterium tuberculosis ADP + phosphorylated-Rv2175
-
 ?
2.7.11.1 ATP + Rv2175
-
 Mycobacterium tuberculosis H37Rv ADP + phosphorylated-Rv2175
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.1 PknL
-
 Mycobacterium tuberculosis
2.7.11.1 serine-threonine kinase
-
 Mycobacterium tuberculosis