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Literature summary extracted from

  • Rodriguez-Zavala, J.S.
    Enhancement of coenzyme binding by a single point mutation at the coenzyme binding domain of E. coli lactaldehyde dehydrogenase (2008), Protein Sci., 17, 563-570.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.22 vectors containing His-Tag constructs overexpressed in Escherichia coli BL21 (DE3)pLysS strain Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.22 F180T renders an enzyme with the ability to use NADP+. NADP+ activity is higher than that attained with NAD+. Exhibits a 16fold increase in the Vm/Km ratio with NAD+ as the coenzyme. Absence of Mg2+ inhibitory effect on F180T activity Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.22 Mg2+
-
Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.22 0.007
-
NAD+ mutant F180T Escherichia coli
1.2.1.22 0.0078
-
NADP+ mutant F180T Escherichia coli
1.2.1.22 0.047
-
NAD+ wild-type Escherichia coli
1.2.1.22 0.25
-
propionaldehyde wild-type Escherichia coli
1.2.1.22 0.26
-
propionaldehyde mutant F180T Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.22 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.22 mutant F180T purified to homogeneity (more than 95% pure), on Ni2+ resin Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.2.1.22 -20°C, 100 mM H2NaPO4, pH 7.5, 100 mM NaCl,0.025% 2-mercaptoethanol, 50% glycerol, stable for 6 months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.22 benzaldehyde + NAD+ + H2O
-
Escherichia coli benzoate + NADH + H+
-
?
1.2.1.22 p-methoxybenzaldehyde + NAD+ + H2O
-
Escherichia coli p-methoxybenzoate + NADH + H+
-
?
1.2.1.22 p-nitrobenzaldehyde + NAD+ + H2O
-
Escherichia coli p-nitrobenzoate + NADH + H+
-
?
1.2.1.22 propionaldehyde + NAD+ + H2O
-
Escherichia coli propionate + NADH + H+
-
?
1.2.1.22 propionaldehyde + NADP+ + H2O ALD wild-type only uses NAD+. F180T mutation renders an enzyme with the ability to use NADP+ Escherichia coli propionate + NADPH + H+
-
?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.22 ALD
-
Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.22 NAD+ ALD only uses NAD+. Residue F180 of ALD may participate in coenzyme specificity Escherichia coli