BRENDA - Enzyme Database

Mechanisms of substrate selectivity for Bacillus anthracis thymidylate kinase

Carnrot, C.; Wang, L.; Topalis, D.; Eriksson, S.; Protein Sci. 17, 1486-1493 (2008)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
2.7.4.9
structural model based on staphylococcus aureus structure
Bacillus anthracis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.4.9
2',3'-dideoxy-2',3'-didehydrothymidine
poor inhibitor
Bacillus anthracis
2.7.4.9
3'-azidothymidine
poor inhibitor
Bacillus anthracis
2.7.4.9
5-fluorodeoxyuridine
poor inhibitor
Bacillus anthracis
2.7.4.9
D-2'-fluoroarabinosyl-5-methyldeoxyuridine
poor inhibitor; strong
Bacillus anthracis
2.7.4.9
deoxythymidine
poor inhibitor
Bacillus anthracis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.9
0.0044
-
D-2'-fluoroarabinosyl-5-methyldeoxyuridine 5'-monophosphate
pH 7.6, 37°C
Bacillus anthracis
2.7.4.9
0.033
-
dTMP
-
Bacillus anthracis
2.7.4.9
0.167
-
L-2'-fluoroarabinosyl-5-methyldeoxyuridine 5'-monophosphate
pH 7.6, 37°C
Bacillus anthracis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.4.9
Bacillus anthracis
Q81W11
-
-
Reaction
EC Number
Reaction
Commentary
Organism
2.7.4.9
ATP + dTMP = ADP + dTDP
binding of D-dTMP is through a network of hydrogen bonds to the pyrimidine ring involving residues R70, S97, and Q101, and the base is stacked by residue Y66. The phosphate group is tightly coordinated by residues R34, D91, and R92
Bacillus anthracis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.9
ATP + 2',3'-dideoxy-2',3'-didehydrothymidine 5'-monophosphate
-
695005
Bacillus anthracis
ADP + 2',3'-dideoxy-2',3'-didehydrothymidine 5'-diphosphate
-
-
-
?
2.7.4.9
ATP + 5-fluorodeoxyuridine 5'-monophosphate
poor substrate
695005
Bacillus anthracis
ADP + 5-fluorodeoxyuridine 5'-diphosphate
-
-
-
?
2.7.4.9
ATP + AMP
3.7% of the activity with dTMP
695005
Bacillus anthracis
ADP + ADP
-
-
-
?
2.7.4.9
ATP + D-2'-fluoroarabinosyl-5-methyldeoxyuridine 5'-monophosphate
172% of the activity with dTMP
695005
Bacillus anthracis
ADP + D-2'-fluoroarabinosyl-5-methyldeoxyuridine 5'-diphosphate
-
-
-
?
2.7.4.9
ATP + dAMP
2.7% of the activity with dTMP
695005
Bacillus anthracis
ADP + dADP
-
-
-
?
2.7.4.9
ATP + dCMP
1% of the activity with dTMP
695005
Bacillus anthracis
ADP + dCDP
-
-
-
?
2.7.4.9
ATP + dTMP
-
695005
Bacillus anthracis
ADP + dTDP
-
-
-
?
2.7.4.9
ATP + dUMP
10.7% of the activity with dTMP
695005
Bacillus anthracis
ADP + dUDP
-
-
-
?
2.7.4.9
ATP + IMP
1% of the activity with dTMP
695005
Bacillus anthracis
ADP + IDP
-
-
-
?
2.7.4.9
ATP + L-2'-fluoroarabinosyl-5-methyldeoxyuridine 5'-monophosphate
poor substrate
695005
Bacillus anthracis
ADP + L-2'-fluoroarabinosyl-5-methyldeoxyuridine 5'-diphosphate
-
-
-
?
2.7.4.9
dATP + dTMP
dATP, 83% of the activity with ATP
695005
Bacillus anthracis
dADP + dTDP
-
-
-
?
2.7.4.9
additional information
no substrate: 3'-azidothymidine 5'-monophosphate
695005
Bacillus anthracis
?
-
-
-
-
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
2.7.4.9
0.01
-
pH 7.6, 37°C
Bacillus anthracis
D-2'-fluoroarabinosyl-5-methyldeoxyuridine
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
2.7.4.9
structural model based on staphylococcus aureus structure
Bacillus anthracis
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
2.7.4.9
0.01
-
pH 7.6, 37°C
Bacillus anthracis
D-2'-fluoroarabinosyl-5-methyldeoxyuridine
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.4.9
2',3'-dideoxy-2',3'-didehydrothymidine
poor inhibitor
Bacillus anthracis
2.7.4.9
3'-azidothymidine
poor inhibitor
Bacillus anthracis
2.7.4.9
5-fluorodeoxyuridine
poor inhibitor
Bacillus anthracis
2.7.4.9
D-2'-fluoroarabinosyl-5-methyldeoxyuridine
poor inhibitor; strong
Bacillus anthracis
2.7.4.9
deoxythymidine
poor inhibitor
Bacillus anthracis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.9
0.0044
-
D-2'-fluoroarabinosyl-5-methyldeoxyuridine 5'-monophosphate
pH 7.6, 37°C
Bacillus anthracis
2.7.4.9
0.033
-
dTMP
-
Bacillus anthracis
2.7.4.9
0.167
-
L-2'-fluoroarabinosyl-5-methyldeoxyuridine 5'-monophosphate
pH 7.6, 37°C
Bacillus anthracis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.9
ATP + 2',3'-dideoxy-2',3'-didehydrothymidine 5'-monophosphate
-
695005
Bacillus anthracis
ADP + 2',3'-dideoxy-2',3'-didehydrothymidine 5'-diphosphate
-
-
-
?
2.7.4.9
ATP + 5-fluorodeoxyuridine 5'-monophosphate
poor substrate
695005
Bacillus anthracis
ADP + 5-fluorodeoxyuridine 5'-diphosphate
-
-
-
?
2.7.4.9
ATP + AMP
3.7% of the activity with dTMP
695005
Bacillus anthracis
ADP + ADP
-
-
-
?
2.7.4.9
ATP + D-2'-fluoroarabinosyl-5-methyldeoxyuridine 5'-monophosphate
172% of the activity with dTMP
695005
Bacillus anthracis
ADP + D-2'-fluoroarabinosyl-5-methyldeoxyuridine 5'-diphosphate
-
-
-
?
2.7.4.9
ATP + dAMP
2.7% of the activity with dTMP
695005
Bacillus anthracis
ADP + dADP
-
-
-
?
2.7.4.9
ATP + dCMP
1% of the activity with dTMP
695005
Bacillus anthracis
ADP + dCDP
-
-
-
?
2.7.4.9
ATP + dTMP
-
695005
Bacillus anthracis
ADP + dTDP
-
-
-
?
2.7.4.9
ATP + dUMP
10.7% of the activity with dTMP
695005
Bacillus anthracis
ADP + dUDP
-
-
-
?
2.7.4.9
ATP + IMP
1% of the activity with dTMP
695005
Bacillus anthracis
ADP + IDP
-
-
-
?
2.7.4.9
ATP + L-2'-fluoroarabinosyl-5-methyldeoxyuridine 5'-monophosphate
poor substrate
695005
Bacillus anthracis
ADP + L-2'-fluoroarabinosyl-5-methyldeoxyuridine 5'-diphosphate
-
-
-
?
2.7.4.9
dATP + dTMP
dATP, 83% of the activity with ATP
695005
Bacillus anthracis
dADP + dTDP
-
-
-
?
2.7.4.9
additional information
no substrate: 3'-azidothymidine 5'-monophosphate
695005
Bacillus anthracis
?
-
-
-
-