BRENDA - Enzyme Database

Recombinant human serine racemase: enzymologic characterization and comparison with its mouse ortholog

Hoffman, H.E.; Jiraskova, J.; Ingr, M.; Zvelebil, M.; Konvalinka, J.; Protein Expr. Purif. 63, 62-67 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.1.1.18
optimization for expression in Escherichia coli
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.1.1.18
glycine
-
Homo sapiens
5.1.1.18
glycine
-
Mus musculus
5.1.1.18
L-erythro-3-hydroxyaspartate
-
Homo sapiens
5.1.1.18
L-erythro-3-hydroxyaspartate
-
Mus musculus
5.1.1.18
malonate
-
Mus musculus
5.1.1.18
malonate
-
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.1.1.18
3.2
-
D-serine
pH 8.0
Mus musculus
5.1.1.18
3.8
-
L-serine
pH 8.0
Mus musculus
5.1.1.18
4.1
-
L-serine
pH 8.0
Homo sapiens
5.1.1.18
10.8
-
D-serine
pH 8.0
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.1.1.18
Homo sapiens
-
-
-
5.1.1.18
Mus musculus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.1.1.18
recombinant enzyme
Homo sapiens
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.1.1.18
16.2
-
pH 8.0
Homo sapiens
5.1.1.18
16.2
-
pH 8.0
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.1.1.18
L-serine
-
694974
Mus musculus
D-serine
-
-
-
r
5.1.1.18
L-serine
-
694974
Homo sapiens
D-serine
-
-
-
r
5.1.1.18
additional information
enzyme additionally catalyzes eleimination reaction of D-/L-serine and of L-serine-O-sulfate
694974
Mus musculus
?
-
-
-
-
5.1.1.18
additional information
enzyme additionally catalyzes elimination reaction of D-/L-serine and of L-serine-O-sulfate
694974
Homo sapiens
?
-
-
-
-
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.1.1.18
0.69
-
L-serine
pH 8.0
Homo sapiens
5.1.1.18
0.78
-
L-serine
pH 8.0
Mus musculus
5.1.1.18
1.41
-
D-serine
pH 8.0
Homo sapiens
5.1.1.18
1.88
-
D-serine
pH 8.0
Mus musculus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
5.1.1.18
0.011
-
L-erythro-3-hydroxyaspartate
-
Homo sapiens
5.1.1.18
0.033
-
malonate
-
Homo sapiens
5.1.1.18
0.043
-
L-erythro-3-hydroxyaspartate
-
Mus musculus
5.1.1.18
0.071
-
malonate
-
Mus musculus
5.1.1.18
0.366
-
glycine
-
Homo sapiens
5.1.1.18
1.64
-
glycine
-
Mus musculus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.1.1.18
optimization for expression in Escherichia coli
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.1.1.18
glycine
-
Homo sapiens
5.1.1.18
glycine
-
Mus musculus
5.1.1.18
L-erythro-3-hydroxyaspartate
-
Homo sapiens
5.1.1.18
L-erythro-3-hydroxyaspartate
-
Mus musculus
5.1.1.18
malonate
-
Homo sapiens
5.1.1.18
malonate
-
Mus musculus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
5.1.1.18
0.011
-
L-erythro-3-hydroxyaspartate
-
Homo sapiens
5.1.1.18
0.033
-
malonate
-
Homo sapiens
5.1.1.18
0.043
-
L-erythro-3-hydroxyaspartate
-
Mus musculus
5.1.1.18
0.071
-
malonate
-
Mus musculus
5.1.1.18
0.366
-
glycine
-
Homo sapiens
5.1.1.18
1.64
-
glycine
-
Mus musculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.1.1.18
3.2
-
D-serine
pH 8.0
Mus musculus
5.1.1.18
3.8
-
L-serine
pH 8.0
Mus musculus
5.1.1.18
4.1
-
L-serine
pH 8.0
Homo sapiens
5.1.1.18
10.8
-
D-serine
pH 8.0
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.1.1.18
recombinant enzyme
Homo sapiens
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.1.1.18
16.2
-
pH 8.0
Homo sapiens
5.1.1.18
16.2
-
pH 8.0
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.1.1.18
L-serine
-
694974
Mus musculus
D-serine
-
-
-
r
5.1.1.18
L-serine
-
694974
Homo sapiens
D-serine
-
-
-
r
5.1.1.18
additional information
enzyme additionally catalyzes eleimination reaction of D-/L-serine and of L-serine-O-sulfate
694974
Mus musculus
?
-
-
-
-
5.1.1.18
additional information
enzyme additionally catalyzes elimination reaction of D-/L-serine and of L-serine-O-sulfate
694974
Homo sapiens
?
-
-
-
-
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.1.1.18
0.69
-
L-serine
pH 8.0
Homo sapiens
5.1.1.18
0.78
-
L-serine
pH 8.0
Mus musculus
5.1.1.18
1.41
-
D-serine
pH 8.0
Homo sapiens
5.1.1.18
1.88
-
D-serine
pH 8.0
Mus musculus