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Literature summary extracted from
- Biophysical and biochemical characterization of reconstituted and purified Rhodobacter sphaeroides cytochrome c oxidase in phospholipid vesicles sheds insight into its functional oligomeric structure (2007), Protein Expr. Purif., 56, 189-196.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 7.1.1.9 | additional information | the monomeric form of Rhodobacter sphaeroides COX when reconstituted into a phospholipid bilayer is completely functionally active in its ability to perform electron transfer and proton pumping activities of the enzyme | Cereibacter sphaeroides |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 7.1.1.9 | mitochondrion | - |
Cereibacter sphaeroides | 5739 | - |
| 7.1.1.9 | additional information | cytochrome oxidase unilamellar vesicles (COV). Purified COV contains approximately 1 COX molecule per liposome. Purified COV exhibits similar physical properties as unpurified COV | Cereibacter sphaeroides | - |
- |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.1.1.9 | Cereibacter sphaeroides | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.1.1.9 | polyhistidine-labeled protein purified on a Ni-NTA column | Cereibacter sphaeroides |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.1.1.9 | ferrocytochrome c + O2 + H+ | - |
Cereibacter sphaeroides | ferricytochrome c + H2O | - |
r |  |
| 7.1.1.9 | reduced cytochrome c + O2 + H+ | - |
Cereibacter sphaeroides | oxidized cytochrome c + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 7.1.1.9 | monomer | - |
Cereibacter sphaeroides |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.1.1.9 | COX | - |
Cereibacter sphaeroides |
| 7.1.1.9 | cytochrome c oxidase | - |
Cereibacter sphaeroides |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.1.1.9 | 535 | - |
ferrocytochrome c | purified COV | Cereibacter sphaeroides | |
| 7.1.1.9 | 708 | - |
ferrocytochrome c | unpurified COV | Cereibacter sphaeroides | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.1.9 | heme | heme aa3 | Cereibacter sphaeroides | |
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Release 2023.1 (January 2023)
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