Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhao, L.; Xu, J.; Zhao, J.; Pan, J.; Wang, Z.
    Biochemical properties and potential applications of an organic solvent-tolerant lipase isolated from Serratia marcescens ECU1010 (2008), Process Biochem., 43, 626-633.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 Gum arabic 142% relative activity at 1% (w/v) Serratia marcescens
3.1.1.3 Pg350me 117% relative activity at 1% (w/v) Serratia marcescens
3.1.1.3 polyvinyl alcohol 118% relative activity at 1% (w/v) Serratia marcescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 acetone 98.3% inhibition at 50% (v/v) Serratia marcescens
3.1.1.3 Ca2+ 95.0% residual activity at 10 mM Serratia marcescens
3.1.1.3 Co2+ 46.0% residual activity at 10 mM Serratia marcescens
3.1.1.3 Cu2+ 37.0% residual activity at 10 mM Serratia marcescens
3.1.1.3 EDTA 16.0% residual activity at 10 mM Serratia marcescens
3.1.1.3 ethanol 98.8% inhibition at 50% (v/v) Serratia marcescens
3.1.1.3 Fe2+ 14.0% residual activity at 10 mM Serratia marcescens
3.1.1.3 Fe3+ 12% residual activity at 10 mM Serratia marcescens
3.1.1.3 Isopropanol 98.9% inhibition at 50% (v/v) Serratia marcescens
3.1.1.3 K+ 89.0% residual activity at 10 mM Serratia marcescens
3.1.1.3 methanol 99% inhibition at 50% (v/v) Serratia marcescens
3.1.1.3 Mg2+ 88.0% residual activity at 10 mM Serratia marcescens
3.1.1.3 Mn2+ 60.0% residual activity at 10 mM Serratia marcescens
3.1.1.3 Na+ 90.0% residual activity at 10 mM Serratia marcescens
3.1.1.3 Ni2+ 40.0% residual activity at 10 mM Serratia marcescens
3.1.1.3 Pg250de 78% residual activity at 1% (v/v) Serratia marcescens
3.1.1.3 Pg400de 96% residual activity at 1% (v/v) Serratia marcescens
3.1.1.3 Triton X-100 inhibits the activity seriously Serratia marcescens
3.1.1.3 Triton X-45 inhibits the activity seriously Serratia marcescens
3.1.1.3 Tween 80 72% residual activity at 1% (v/v) Serratia marcescens
3.1.1.3 Zn2+ 16.2% residual activity at 10 mM Serratia marcescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 additional information not activated by Ca2+ Serratia marcescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 65000
-
 SDS-PAGE Serratia marcescens
3.1.1.3 140000
-
 native lipase, gel filtration Serratia marcescens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Serratia marcescens
-
-
-
3.1.1.3 Serratia marcescens ECU1010
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 ammonium sulfate precipitation, DEAE-Toyopearl 650 M column chromatography, Sephadex G150 gel filtration, and phenyl-Toyopearl 650 M column chromatography Serratia marcescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 90
-
 crude enzyme, using 4-nitrophenyl butyrate as substrate, in 100 mM potassium phosphate buffer (pH 7.0), at 30°C Serratia marcescens
3.1.1.3 497
-
 after 5.5fold purification, using 4-nitrophenyl butyrate as substrate, in 100 mM potassium phosphate buffer (pH 7.0), at 30°C Serratia marcescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-hydroxy-3-methyl-2-(2-propenyl)-2-cyclopenten-1-one acetate + H2O 50% conversion Serratia marcescens ?
-
 ?
3.1.1.3 4-nitrophenyl acetate + H2O
-
 Serratia marcescens 4-nitrophenol + acetate
-
 ?
3.1.1.3 4-nitrophenyl acetate + H2O
-
 Serratia marcescens ECU1010 4-nitrophenol + acetate
-
 ?
3.1.1.3 4-nitrophenyl butyrate + H2O
-
 Serratia marcescens 4-nitrophenol + butyrate
-
 ?
3.1.1.3 4-nitrophenyl butyrate + H2O
-
 Serratia marcescens ECU1010 4-nitrophenol + butyrate
-
 ?
3.1.1.3 4-nitrophenyl decanoate + H2O
-
 Serratia marcescens 4-nitrophenol + decanoate
-
 ?
3.1.1.3 4-nitrophenyl hexanoate + H2O
-
 Serratia marcescens 4-nitrophenol + hexanoate
-
 ?
3.1.1.3 4-nitrophenyl hexanoate + H2O
-
 Serratia marcescens ECU1010 4-nitrophenol + hexanoate
-
 ?
3.1.1.3 4-nitrophenyl laurate + H2O
-
 Serratia marcescens 4-nitrophenol + laurate
-
 ?
3.1.1.3 4-nitrophenyl myristate + H2O the enzyme shows maximum activity on 4-nitrophenyl myristate Serratia marcescens 4-nitrophenol + myristate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Serratia marcescens 4-nitrophenol + palmitate
-
 ?
3.1.1.3 4-nitrophenyl propionate + H2O
-
 Serratia marcescens 4-nitrophenol + propionate
-
 ?
3.1.1.3 4-nitrophenyl propionate + H2O
-
 Serratia marcescens ECU1010 4-nitrophenol + propionate
-
 ?
3.1.1.3 4-phenyl-[1,3]-dioxolan-2-one + H2O 9.6% conversion Serratia marcescens ?
-
 ?
3.1.1.3 glycidyl butyrate + H2O 49% conversion Serratia marcescens ?
-
 ?
3.1.1.3 methyl acetate + H2O 25% conversion Serratia marcescens ?
-
 ?
3.1.1.3 methyl mandelate + H2O 33% conversion Serratia marcescens ?
-
 ?
3.1.1.3 additional information does not hydolyze propylene carbonate Serratia marcescens ?
-
 ?
3.1.1.3 additional information does not hydolyze propylene carbonate Serratia marcescens ECU1010 ?
-
 ?
3.1.1.3 naproxen methyl ester + H2O 41% conversion Serratia marcescens ?
-
 ?
3.1.1.3 trans-3-(4'-methoxyphenyl) glycidic acid methyl ester + H2O 60% conversion Serratia marcescens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 homodimer 2 * 65000, native lipase, gel filtration Serratia marcescens

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Serratia marcescens
3.1.1.3 triacylglycerol hydrolase
-
 Serratia marcescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 50
-
 retains 71% activity after incubation at 50°C for 1 h Serratia marcescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8
-
-
 Serratia marcescens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.3 6 9
-
 Serratia marcescens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.3 Serratia marcescens isoelectric focusing
-
 4.2