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Literature summary extracted from

  • Paukstelis, P.J.; Lambowitz, A.M.
    Identification and evolution of fungal mitochondrial tyrosyl-tRNA synthetases with group I intron splicing activity (2008), Proc. Natl. Acad. Sci. USA, 105, 6010-6015.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.1 expressed in Escherichia coli as a His-tagged fusion protein Aspergillus nidulans
6.1.1.1 expressed in Escherichia coli as a His-tagged fusion protein Histoplasma capsulatum
6.1.1.1 expressed in Escherichia coli as a His-tagged fusion protein Coccidioides posadasii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.1 mitochondrion
-
 Aspergillus nidulans 5739
-
6.1.1.1 mitochondrion
-
 Histoplasma capsulatum 5739
-
6.1.1.1 mitochondrion
-
 Coccidioides posadasii 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.1 Mg2+
-
 Aspergillus nidulans
6.1.1.1 Mg2+
-
 Histoplasma capsulatum
6.1.1.1 Mg2+
-
 Coccidioides posadasii

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.1 Aspergillus nidulans
-
-
-
6.1.1.1 Coccidioides posadasii
-
-
-
6.1.1.1 Histoplasma capsulatum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.1 using Ni-NTA chromatography Aspergillus nidulans
6.1.1.1 using Ni-NTA chromatography Histoplasma capsulatum
6.1.1.1 using Ni-NTA chromatography Coccidioides posadasii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.1 additional information
-
 enzymatic activity is shown Aspergillus nidulans
6.1.1.1 additional information
-
 enzymatic activity is shown Histoplasma capsulatum
6.1.1.1 additional information
-
 enzymatic activity is shown Coccidioides posadasii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.1 ATP + L-tyrosine + tRNATyr
-
 Aspergillus nidulans AMP + diphosphate + L-tyrosyl-tRNATyr
-
 ?
6.1.1.1 ATP + L-tyrosine + tRNATyr
-
 Histoplasma capsulatum AMP + diphosphate + L-tyrosyl-tRNATyr
-
 ?
6.1.1.1 ATP + L-tyrosine + tRNATyr
-
 Coccidioides posadasii AMP + diphosphate + L-tyrosyl-tRNATyr
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.1 mitochondrial tyrosyl-tRNA synthetase
-
 Aspergillus nidulans
6.1.1.1 mitochondrial tyrosyl-tRNA synthetase
-
 Histoplasma capsulatum
6.1.1.1 mitochondrial tyrosyl-tRNA synthetase
-
 Coccidioides posadasii
6.1.1.1 mtTyrRS bifunctional mitochondrial tyrosyl-tRNA synthetase (CYT-18 protein) both aminoacylates mitochondrial tRNATyr and acts as a structure-stabilizing splicing cofactor for group I introns Aspergillus nidulans
6.1.1.1 mtTyrRS bifunctional mitochondrial tyrosyl-tRNA synthetase (CYT-18 protein) both aminoacylates mitochondrial tRNATyr and acts as a structure-stabilizing splicing cofactor for group I introns Histoplasma capsulatum
6.1.1.1 mtTyrRS bifunctional mitochondrial tyrosyl-tRNA synthetase (CYT-18 protein) both aminoacylates mitochondrial tRNATyr and acts as a structure-stabilizing splicing cofactor for group I introns Coccidioides posadasii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.1 30
-
 assay at Aspergillus nidulans
6.1.1.1 30
-
 assay at Histoplasma capsulatum
6.1.1.1 30
-
 assay at Coccidioides posadasii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.1 7.5
-
 assay at Aspergillus nidulans
6.1.1.1 7.5
-
 assay at Histoplasma capsulatum
6.1.1.1 7.5
-
 assay at Coccidioides posadasii