BRENDA - Enzyme Database show

Structural basis for induced formation of the inflammatory mediator prostaglandin E2

Jegerschoeld, C.; Pawelzik, S.C.; Purhonen, P.; Bhakat, P.; Gheorghe, K.R.; Gyobu, N.; Mitsuoka, K.; Morgenstern, R.; Jakobsson, P.J.; Hebert, H.; Proc. Natl. Acad. Sci. USA 105, 11110-11115 (2008)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
5.3.99.3
medicine
MPGES1 has been implicated in a number of human diseases or pathological conditions, such as rheumatoid arthritis, fever, and pain, and is therefore regarded as a primary target for development of novel antiinflammatory drugs
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
5.3.99.3
expression in Escherichia coli cells
Homo sapiens
Crystallization (Commentary)
EC Number
Crystallization
Organism
5.3.99.3
the structure of human MPGES1 is determined in complex with the tripeptide gamma-L-glutamyl-L-cysteinyl-glycine, glutathione, at 3.5 A in-plane resolution using electron crystallography
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
5.3.99.3
E66A
mutant, fraction of wild-type activity, 53%
Homo sapiens
5.3.99.3
E77A
mutant, no activity
Homo sapiens
5.3.99.3
H72A
mutant, fraction of wild-type activity, 32%
Homo sapiens
5.3.99.3
R110A
mutant, no activity
Homo sapiens
5.3.99.3
R110S
mutant, no activity
Homo sapiens
5.3.99.3
R67A
mutant, no activity
Homo sapiens
5.3.99.3
R70A
mutant, fraction of wild-type activity, 58%
Homo sapiens
5.3.99.3
R70A/Y117A
mutant, fraction of wild-type activity, 7%
Homo sapiens
5.3.99.3
R70S
mutant, full activity
Homo sapiens
5.3.99.3
Y117A
mutant, fraction of wild-type activity, 1%
Homo sapiens
5.3.99.3
Y117F
mutant, full activity
Homo sapiens
5.3.99.3
Y130I
mutant, fraction of wild-type activity, 15%
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.3
membrane
-
Homo sapiens
16020
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.3
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
Homo sapiens
-
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.3
Homo sapiens
O14684
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.99.3
-
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.3
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
694863
Homo sapiens
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
5.3.99.3
homotrimer
-
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.3.99.3
glutathione
-
Homo sapiens
Application (protein specific)
EC Number
Application
Commentary
Organism
5.3.99.3
medicine
MPGES1 has been implicated in a number of human diseases or pathological conditions, such as rheumatoid arthritis, fever, and pain, and is therefore regarded as a primary target for development of novel antiinflammatory drugs
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.3
expression in Escherichia coli cells
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.3.99.3
glutathione
-
Homo sapiens
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
5.3.99.3
the structure of human MPGES1 is determined in complex with the tripeptide gamma-L-glutamyl-L-cysteinyl-glycine, glutathione, at 3.5 A in-plane resolution using electron crystallography
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
5.3.99.3
E66A
mutant, fraction of wild-type activity, 53%
Homo sapiens
5.3.99.3
E77A
mutant, no activity
Homo sapiens
5.3.99.3
H72A
mutant, fraction of wild-type activity, 32%
Homo sapiens
5.3.99.3
R110A
mutant, no activity
Homo sapiens
5.3.99.3
R110S
mutant, no activity
Homo sapiens
5.3.99.3
R67A
mutant, no activity
Homo sapiens
5.3.99.3
R70A
mutant, fraction of wild-type activity, 58%
Homo sapiens
5.3.99.3
R70A/Y117A
mutant, fraction of wild-type activity, 7%
Homo sapiens
5.3.99.3
R70S
mutant, full activity
Homo sapiens
5.3.99.3
Y117A
mutant, fraction of wild-type activity, 1%
Homo sapiens
5.3.99.3
Y117F
mutant, full activity
Homo sapiens
5.3.99.3
Y130I
mutant, fraction of wild-type activity, 15%
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.3
membrane
-
Homo sapiens
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.3
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
Homo sapiens
-
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.3
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.3
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
694863
Homo sapiens
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.3.99.3
homotrimer
-
Homo sapiens