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Literature summary extracted from

  • Gaur, R.; Gupta, A.; Khare, S.K.
    Purification and characterization of lipase from solvent tolerant Pseudomonas aeruginosa PseA (2008), Proc. Biochem., 43, 1040-1046.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 Fe3+ 132% relative activity after 30 min at 1 mM Pseudomonas aeruginosa
3.1.1.3 glutathione 112% relative activity after 30 min at 1 mM Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 2-mercaptoethanol reduces the enzyme activity to 48% after 1 h Pseudomonas aeruginosa
3.1.1.3 cetyltrimethylammonium bromide 45% residual activity after 5 h at 25% (v/v) Pseudomonas aeruginosa
3.1.1.3 CHAPS 86% residual activity after 5 h at 25% (v/v) Pseudomonas aeruginosa
3.1.1.3 Cu2+ 70% residual activity after 30 min at 8 mM Pseudomonas aeruginosa
3.1.1.3 diisopropylfluorophosphate 82% residual activity after 30 min at 8 mM Pseudomonas aeruginosa
3.1.1.3 hydrogen peroxide 62% residual activity after 5 h at 25% (v/v) Pseudomonas aeruginosa
3.1.1.3 additional information the enzyme activity is not affected significantly by Hg2+, Ca2+, Mg2+, Fe3+, EDTA and phenylmethylsulfonyl fluoride (1 mM each) Pseudomonas aeruginosa
3.1.1.3 p-chloromercuric benzoate 20% residual activity after 30 min at 8 mM Pseudomonas aeruginosa
3.1.1.3 sodium deoxycholate 90% residual activity after 5 h at 25% (v/v) Pseudomonas aeruginosa
3.1.1.3 sodium hypochlorite 85% residual activity after 5 h at 25% (v/v) Pseudomonas aeruginosa
3.1.1.3 Tween 20 74% residual activity after 5 h at 25% (v/v) Pseudomonas aeruginosa
3.1.1.3 Zn2+ 51% residual activity after 30 min at 1 mM Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.3 70.4
-
 4-nitrophenyl palmitate in 0.1 M Tris-HCl buffer (pH 8.0), at 40°C Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 60000
-
 SDS-PAGE Pseudomonas aeruginosa

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.3 Brij 35 121% relative activity after 5 h at 25% (v/v) Pseudomonas aeruginosa
3.1.1.3 additional information the enzyme activity is not significantly affected by dimethylsulfonyloxide, isopropanol, ethanol, methanol, benzene, toluene, xylene, cyclohexane, hexane, n-heptane, isooctane, decane, and tetradecane Pseudomonas aeruginosa
3.1.1.3 Tween 80 124% relative activity after 5 h at 25% (v/v) Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Pseudomonas aeruginosa
-
-
-
3.1.1.3 Pseudomonas aeruginosa PseA
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 ultrafiltration and Sephadex G-100 gel filtration Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 16.6
-
 crude filtrate, in Tris-HCl, buffer (0.1 M, pH 8.0) Pseudomonas aeruginosa
3.1.1.3 143.3
-
 after 8.6fold purification, in Tris-HCl, buffer (0.1 M, pH 8.0) Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 3 triolein + 3 H2O
-
 Pseudomonas aeruginosa 1,3-diolein + 1,2-diolein + 2,3-diolein + 3 oleate shows random positional specificity for triolein hydrolysis ?
3.1.1.3 4-nitrophenyl acetate + H2O
-
 Pseudomonas aeruginosa 4-nitrophenol + acetate
-
 ?
3.1.1.3 4-nitrophenyl acetate + H2O
-
 Pseudomonas aeruginosa PseA 4-nitrophenol + acetate
-
 ?
3.1.1.3 4-nitrophenyl butyrate + H2O
-
 Pseudomonas aeruginosa 4-nitrophenol + butyrate
-
 ?
3.1.1.3 4-nitrophenyl butyrate + H2O
-
 Pseudomonas aeruginosa PseA 4-nitrophenol + butyrate
-
 ?
3.1.1.3 4-nitrophenyl caprate + H2O
-
 Pseudomonas aeruginosa 4-nitrophenol + caprate
-
 ?
3.1.1.3 4-nitrophenyl caprate + H2O
-
 Pseudomonas aeruginosa PseA 4-nitrophenol + caprate
-
 ?
3.1.1.3 4-nitrophenyl myristate + H2O
-
 Pseudomonas aeruginosa 4-nitrophenol + myristate
-
 ?
3.1.1.3 4-nitrophenyl myristate + H2O
-
 Pseudomonas aeruginosa PseA 4-nitrophenol + myristate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Pseudomonas aeruginosa 4-nitrophenol + palmitate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Pseudomonas aeruginosa PseA 4-nitrophenol + palmitate
-
 ?
3.1.1.3 4-nitrophenyl stearate + H2O
-
 Pseudomonas aeruginosa 4-nitrophenol + stearate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 40
-
-
 Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 25 50
-
 Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8
-
-
 Pseudomonas aeruginosa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.3 6 8.5
-
 Pseudomonas aeruginosa