Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hunter, J.H.; Gujjar, R.; Pang, C.K.; Rathod, P.K.
    Kinetics and ligand-binding preferences of Mycobacterium tuberculosis thymidylate synthases, ThyA and ThyX (2008), PLoS ONE, 3, e2237.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.1.1.45 medicine thymidylate synthase as a target for antitubercular drugs Mycobacterium tuberculosis
2.1.1.148 medicine thymidylate synthase as a target for antitubercular drugs Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.45 expression in Escherichia coli Mycobacterium tuberculosis
2.1.1.45 histidine-tagged Mycobacterium tuberculosis ThyA is overexpressed from BL21-DE3 pLysS Escherichia coli Mycobacterium tuberculosis
2.1.1.148 histidine-tagged Mycobacterium tuberculosis ThyX is overexpressed from BL21-DE3 pLysS Escherichia coli Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 1843U89 i.e. (2R)-2-(5-[[(1-hydroxy-3-methyl-5,10a-dihydrobenzo[f]quinazolin-9-yl)methyl]amino]-1-oxo-1,3-dihydro-2H-isoindol-2-yl)butanedioic acid, folate-based inhibitor Mycobacterium tuberculosis
2.1.1.45 5-fluoro-2'-deoxyuridine 5'-monophosphate FdUMP Mycobacterium tuberculosis
2.1.1.45 GW1843U89
-
 Mycobacterium tuberculosis
2.1.1.148 5-fluoro-2'-deoxyuridine 5'-monophosphate FdUMP Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.45 0.003
-
 dUMP 22°C Mycobacterium tuberculosis
2.1.1.45 0.004
-
 dUMP
-
 Mycobacterium tuberculosis
2.1.1.45 0.004
-
 5,10-methylenetetrahydrofolate 22°C Mycobacterium tuberculosis
2.1.1.45 0.004
-
 dUMP 22°C Mycobacterium tuberculosis
2.1.1.45 0.07
-
 5,10-methylenetetrahydrofolate
-
 Mycobacterium tuberculosis
2.1.1.45 0.07
-
 5,10-methylenetetrahydrofolate 22°C Mycobacterium tuberculosis
2.1.1.148 0.003
-
 dUMP
-
 Mycobacterium tuberculosis
2.1.1.148 0.004
-
 5,10-methylenetetrahydrofolate
-
 Mycobacterium tuberculosis
2.1.1.148 0.047
-
 NADPH
-
 Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.45 28700
-
 4 * 28700, calculated Mycobacterium tuberculosis
2.1.1.45 30700
-
 2 * 30700, calculated Mycobacterium tuberculosis
2.1.1.45 57000
-
 gel filtration Mycobacterium tuberculosis
2.1.1.45 104000
-
 gel filtration Mycobacterium tuberculosis
2.1.1.148 104000
-
 gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Mycobacterium tuberculosis
-
 7,8-dihydrofolate + dTMP
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Mycobacterium tuberculosis
-
 dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + NADPH Mycobacterium tuberculosis Rhodobacter capsulatus ThyX is required for de novo thymidylate synthesis dTMP + tetrahydrofolate + NADP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Mycobacterium tuberculosis
-
-
-
2.1.1.45 Mycobacterium tuberculosis
-
 isoform ThyX
-
2.1.1.45 Mycobacterium tuberculosis P9WFR9 isoform ThyA
-
2.1.1.45 Mycobacterium tuberculosis H37Rv P9WFR9 isoform ThyA
-
2.1.1.148 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45 nickel affinity, ionexchange, and gel filtration Mycobacterium tuberculosis
2.1.1.45 recombinant protein Mycobacterium tuberculosis
2.1.1.148 nickel affinity and ion-exchange chromatography Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.45 210
-
 22°C, recombinant protein Mycobacterium tuberculosis
2.1.1.45 210
-
 22°C, recombinant protien Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mycobacterium tuberculosis dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mycobacterium tuberculosis H37Rv dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mycobacterium tuberculosis 7,8-dihydrofolate + dTMP
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Mycobacterium tuberculosis dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + NADPH
-
 Mycobacterium tuberculosis dTMP + tetrahydrofolate + NADP
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + NADPH Rhodobacter capsulatus ThyX is required for de novo thymidylate synthesis Mycobacterium tuberculosis dTMP + tetrahydrofolate + NADP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.45 dimer 2 * 30700, calculated Mycobacterium tuberculosis
2.1.1.45 dimer predicted mass of a dimer Mycobacterium tuberculosis
2.1.1.45 tetramer 4 * 28700, calculated Mycobacterium tuberculosis
2.1.1.148 tetramer predicted mass of a tetramer Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.1.1.45 ThyA
-
 Mycobacterium tuberculosis
2.1.1.148 FDTS
-
 Mycobacterium tuberculosis
2.1.1.148 flavin-dependent thymidylate synthase
-
 Mycobacterium tuberculosis
2.1.1.148 ThyX
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.45 0.4
-
 dUMP 22°C Mycobacterium tuberculosis
2.1.1.45 18
-
 dUMP 22°C Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.148 FAD
-
 Mycobacterium tuberculosis
2.1.1.148 NADPH
-
 Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.45 0.000002
-
 5-fluoro-2'-deoxyuridine 5'-monophosphate 22°C Mycobacterium tuberculosis
2.1.1.45 0.000002
-
 5-fluoro-2'-deoxyuridine 5'-monophosphate inhibited both ThyA and ThyX Mycobacterium tuberculosis
2.1.1.45 0.000018
-
 GW1843U89
-
 Mycobacterium tuberculosis
2.1.1.45 0.000018
-
 (2R)-2-(5-[[(1-hydroxy-3-methyl-5,10a-dihydrobenzo[f]quinazolin-9-yl)methyl]amino]-1-oxo-1,3-dihydro-2H-isoindol-2-yl)butanedioic acid 22°C Mycobacterium tuberculosis
2.1.1.45 0.0001
-
 5-fluoro-2'-deoxyuridine 5'-monophosphate 22°C Mycobacterium tuberculosis
2.1.1.148 0.0001
-
 5-fluoro-2'-deoxyuridine 5'-monophosphate inhibited both ThyA and ThyX Mycobacterium tuberculosis