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Literature summary extracted from

  • Bernal, A.J.; Jensen, J.K.; Harholt, J.; S?rensen, S.; Moller, I.; Blaukopf, C.; Johansen, B.; de Lotto, R.; Pauly, M.; Scheller, H.V.; Willats, W.G.
    Disruption of ATCSLD5 results in reduced growth, reduced xylan and homogalacturonan synthase activity and altered xylan occurrence in Arabidopsis (2007), Plant J., 52, 791-802.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.24 genomic DNA from BAC clone T2I1 in (i) pGEMTeasy for expression in Escherichia coli XL1Blue and sequencing, (ii) pCAMBIA2300 for transformation in Agrobacterium tumefaciens GV3101 and floral-flip plant transformation, (iii) pCAMBIA230035SuYFP (C-terminal yellow fluorescent protein (YFP) tag) and (iv) pCAMBIA330035SYFPu (N-terminal YFP tag) for expression and subcellular localization in Nicotiana benthamiana Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.24 Golgi apparatus N-terminal YFP tag (YFP-ATCSLD5), expression in Nicotiana benthamiana, confocal laser scanning, co-localisation with Golgi-marker STtmd-GFP, no co-localisation with endoplasmic reticulum (ER) marker HDEL-GFP, C-terminal fluorescent protein tag leads to mistargeting of ATCSLD5-YFP to ER Arabidopsis thaliana 5794
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Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.24 UDP-D-xylose + (1,4-beta-D-xylan)n Arabidopsis thaliana beta-(1,4)-xylan synthase activity, biosynthesis of xylan (major component of dicot wood) UDP + (1,4-beta-D-xylan)n+1
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 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.24 Arabidopsis thaliana Q9SRW9
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Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.24 microsomal extraction by differential centrifugation using 50 mM HEPES-NaOH pH7.3, 400 microM sucrose, 4 mM dithiothreitol, EDTA for beta-(1,4)-xylan synthase activity measurements Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.24 cambium cell layer of middle stem between second xylem interfasicular region and cortex corresponding to vascular cambium, ATCSLD5 promoter driven beta-glucuronidase (GUS) expression Arabidopsis thaliana
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2.4.2.24 cauline leaf vasicular tissue, ATCSLD5 promoter driven beta-glucuronidase (GUS) expression Arabidopsis thaliana
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2.4.2.24 cotyledon vasicular tissue, ATCSLD5 promoter driven beta-glucuronidase (GUS) expression Arabidopsis thaliana
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2.4.2.24 hypocotyl high levels, otherwise ubiquitous (in silico analysis) Arabidopsis thaliana
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2.4.2.24 additional information absent from flowers and siliques, ATCSLD5 promoter driven beta-glucuronidase (GUS) expression Arabidopsis thaliana
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2.4.2.24 additional information complete loss of ATCSLD5 expression in mutant line atcsld5-1 with T-DNA homozygote insertion in first exon of ATCSLD5 gene, revealed by RT-PCR from 6-week old plants Arabidopsis thaliana
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2.4.2.24 parenchyma weak expression in cortical and pith cells, ATCSLD5 promoter driven beta-glucuronidase (GUS) expression Arabidopsis thaliana
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2.4.2.24 phloem weak expression, ATCSLD5 promoter driven beta-glucuronidase (GUS) expression, possibly false positive due to loading of GUS reaction products via symplastic connections Arabidopsis thaliana
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2.4.2.24 root shorter in absence of ATCSLD5 (ATCSLD5-1 mutant line) compared to wild-type plants, severe reduction in root length in presence of cellulose synthase inhibitor isoxaben abolished by complementation with wild-type ATCSLD5 Arabidopsis thaliana
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2.4.2.24 rosette leaf vasicular tissue, ATCSLD5 promoter driven beta-glucuronidase (GUS) expression Arabidopsis thaliana
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2.4.2.24 shoot tip high levels, otherwise ubiquitous (in silico analysis) Arabidopsis thaliana
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2.4.2.24 stem ATCSLD5-1 mutant line: reaction product 1,4-beta-D-xylan reduction most pronounced in top portion (independent of stem hight), absent from interfasicular tissue as revealed by immunocytochemistry Arabidopsis thaliana
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2.4.2.24 stem reaction product 1,4-beta-D-xylan detectable in top, middle and base portion, present in xylem and interfasicular tissue as revealed by immunocytochemistry, ATCSLD5 most abundant during stem elongation between day 21 and 25 (in silico analysis) Arabidopsis thaliana
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Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.24 additional information
-
 microsomal homogalacturonan synthase activity reduced in absence of ATCSLD5 (ATCSLD5-1 mutant line) Arabidopsis thaliana
2.4.2.24 additional information
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 microsomal xylan synthase activity reduced in absence of ATCSLD5 (ATCSLD5-1 mutant line) Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.24 UDP-D-galacturonic acid + (1,4-beta-D-galacturonic acid)n homogalacturonan synthase activity (substrate: galacturonic acid, GalA) decreased in absence of ATCSLD5 (ATCSLD5-1 mutant line) Arabidopsis thaliana UDP + (1,4-beta-D-galacturonic acid)n+1
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 ?
2.4.2.24 UDP-D-xylose + (1,4-beta-D-xylan)n
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 Arabidopsis thaliana UDP + (1,4-beta-D-xylan)n+1 precipitation of product by 70% ethanol (final concentration) in presence of wheat arabinoxylan, followed by washing and scintillation counting, control for incorporation into (1,4-beta-D-xylan) by digestion with endo-xylanase ?
2.4.2.24 UDP-D-xylose + (1,4-beta-D-xylan)n beta-(1,4)-xylan synthase activity, biosynthesis of xylan (major component of dicot wood) Arabidopsis thaliana UDP + (1,4-beta-D-xylan)n+1
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 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.24 Arabidopsis thaliana cellulose synthase-like gene D family gene 5
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 Arabidopsis thaliana
2.4.2.24 ATCSLD
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 Arabidopsis thaliana
2.4.2.24 ATCSLD5
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 Arabidopsis thaliana