BRENDA - Enzyme Database

Substrate specificity in phospholipid transformations by plant phospholipase D isoenzymes

Dippe, M.; Ulbrich-Hofmann, R.; Phytochemistry 70, 361-365 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.4.4
expressed in Escherichia coli; expressed in Escherichia coli
Brassica oleracea var. capitata
3.1.4.4
expressed in Escherichia coli; expressed in Escherichia coli
Papaver somniferum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.4
Ca2+
;
Brassica oleracea var. capitata
3.1.4.4
Ca2+
;
Papaver somniferum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.4
Brassica oleracea var. capitata
O82549
-
-
3.1.4.4
Brassica oleracea var. capitata
P55939
-
-
3.1.4.4
Papaver somniferum
Q8VWE9
-
-
3.1.4.4
Papaver somniferum
Q8W1B2
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.4
purification by Ca2+-mediated hydrophobic interaction chromatography and an anion exchange chromatography; purification by Ca2+-mediated hydrophobic interaction chromatography and an anion exchange chromatography
Brassica oleracea var. capitata
3.1.4.4
purification by Ca2+-mediated hydrophobic interaction chromatography and an anion exchange chromatography; purification by Ca2+-mediated hydrophobic interaction chromatography and an anion exchange chromatography
Papaver somniferum
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.4
0.21
-
initial rate of transphosphatidylation, acceptor alcohol: D-Ser
Brassica oleracea var. capitata
3.1.4.4
1.88
-
initial rate of transphosphatidylation, acceptor alcohol: L-Ser
Brassica oleracea var. capitata
3.1.4.4
6.3
-
initial rate of hydrolysis, acceptor alcohol: L-Ser
Brassica oleracea var. capitata
3.1.4.4
7.82
-
initial rate of hydrolysis, acceptor alcohol: D-Ser
Brassica oleracea var. capitata
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
phosphatidylcholine + D-serine
for L- and D-serine a stereoselectivity of PLD is observed
694606
Papaver somniferum
phosphatidylserine + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + D-serine
for L- and D-serine a stereoselectivity of PLD is observed
694606
Brassica oleracea var. capitata
phosphatidylserine + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + ethanolamine
preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol
694606
Papaver somniferum
phosphatidylethanolamine + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + ethanolamine
preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-Ser
694606
Brassica oleracea var. capitata
phosphatidylethanolamine + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + ethanolamine
preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-serine
694606
Papaver somniferum
phosphatidylethanolamine + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + ethanolamine
preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-serine
694606
Brassica oleracea var. capitata
phosphatidylethanolamine + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + glycerol
preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol
694606
Papaver somniferum
phosphatidylglycerol + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + glycerol
preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-Ser
694606
Brassica oleracea var. capitata
phosphatidylglycerol + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + glycerol
preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-serine
694606
Papaver somniferum
phosphatidylglycerol + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + glycerol
preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-serine
694606
Brassica oleracea var. capitata
phosphatidylglycerol + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylcholine higher than phosphatidylglycerol equal to phosphatidylethanolamine higher than phosphatidylinositol higher than phosphatidylserine
694606
Brassica oleracea var. capitata
phosphatidate + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol
694606
Papaver somniferum
phosphatidate + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol equal to phosphatidylcholine higher than phosphatidylinositol higher than phosphatidylserine
694606
Brassica oleracea var. capitata
phosphatidate + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol higher than phosphatidylcholine higher than phosphatidylserine higher than phosphatidylinositol
694606
Papaver somniferum
phosphatidate + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + L-serine
for L- and D-serine a stereoselectivity of PLD is observed, preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-serine
694606
Papaver somniferum
phosphatidylserine + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + L-serine
for L- and D-serine a stereoselectivity of PLD is observed, preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-serine
694606
Brassica oleracea var. capitata
phosphatidylserine + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + L-serine
preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-Ser
694606
Brassica oleracea var. capitata
phosphatidylserine + choline
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylcholine higher than phosphatidylglycerol equal to phosphatidylethanolamine higher than phosphatidylinositol higher than phosphatidylserine
694606
Brassica oleracea var. capitata
phosphatidate + ethanolamine
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol
694606
Papaver somniferum
phosphatidate + ethanolamine
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol higher than phosphatidylserine
694606
Brassica oleracea var. capitata
phosphatidate + ethanolamine
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol higher than phosphatidylcholine higher than phosphatidylserine higher than phosphatidylinositol
694606
Papaver somniferum
phosphatidate + ethanolamine
-
-
-
?
3.1.4.4
phosphatidylglycerol + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylcholine higher than phosphatidylglycerol equal to phosphatidylethanolamine higher than phosphatidylinositol higher than phosphatidylserine
694606
Brassica oleracea var. capitata
phosphatidate + glycerol
-
-
-
?
3.1.4.4
phosphatidylglycerol + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol
694606
Papaver somniferum
phosphatidate + glycerol
-
-
-
?
3.1.4.4
phosphatidylglycerol + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol equal to phosphatidylcholine higher than phosphatidylinositol higher than phosphatidylserine
694606
Brassica oleracea var. capitata
phosphatidate + glycerol
-
-
-
?
3.1.4.4
phosphatidylglycerol + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol higher than phosphatidylcholine higher than phosphatidylserine higher than phosphatidylinositol
694606
Papaver somniferum
phosphatidate + glycerol
-
-
-
?
3.1.4.4
phosphatidylinositol + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylcholine higher than phosphatidylglycerol equal to phosphatidylethanolamine higher than phosphatidylinositol higher than phosphatidylserine
694606
Brassica oleracea var. capitata
phosphatidate + inositol
-
-
-
?
3.1.4.4
phosphatidylinositol + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol
694606
Papaver somniferum
phosphatidate + inositol
-
-
-
?
3.1.4.4
phosphatidylinositol + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol higher than phosphatidylserine
694606
Brassica oleracea var. capitata
phosphatidate + inositol
-
-
-
?
3.1.4.4
phosphatidylinositol + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol higher than phosphatidylcholine higher than phosphatidylserine higher than phosphatidylinositol
694606
Papaver somniferum
phosphatidate + inositol
-
-
-
?
3.1.4.4
phosphatidylserine + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylcholine higher than phosphatidylglycerol equal to phosphatidylethanolamine higher than phosphatidylinositol higher than phosphatidylserine
694606
Brassica oleracea var. capitata
phosphatidate + serine
-
-
-
?
3.1.4.4
phosphatidylserine + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol higher than phosphatidylserine
694606
Brassica oleracea var. capitata
phosphatidate + serine
-
-
-
?
3.1.4.4
phosphatidylserine + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol higher than phosphatidylcholine higher than phosphatidylserine higher than phosphatidylinositol
694606
Papaver somniferum
phosphatidate + serine
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.4.4
30
-
assay at; assay at
Brassica oleracea var. capitata
3.1.4.4
30
-
assay at; assay at
Papaver somniferum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
5.5
-
assay at; assay at
Brassica oleracea var. capitata
3.1.4.4
5.5
-
assay at; assay at
Papaver somniferum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.4
expressed in Escherichia coli
Brassica oleracea var. capitata
3.1.4.4
expressed in Escherichia coli
Papaver somniferum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.4
Ca2+
-
Brassica oleracea var. capitata
3.1.4.4
Ca2+
-
Papaver somniferum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.4
purification by Ca2+-mediated hydrophobic interaction chromatography and an anion exchange chromatography
Brassica oleracea var. capitata
3.1.4.4
purification by Ca2+-mediated hydrophobic interaction chromatography and an anion exchange chromatography
Papaver somniferum
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.4
0.21
-
initial rate of transphosphatidylation, acceptor alcohol: D-Ser
Brassica oleracea var. capitata
3.1.4.4
1.88
-
initial rate of transphosphatidylation, acceptor alcohol: L-Ser
Brassica oleracea var. capitata
3.1.4.4
6.3
-
initial rate of hydrolysis, acceptor alcohol: L-Ser
Brassica oleracea var. capitata
3.1.4.4
7.82
-
initial rate of hydrolysis, acceptor alcohol: D-Ser
Brassica oleracea var. capitata
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
phosphatidylcholine + D-serine
for L- and D-serine a stereoselectivity of PLD is observed
694606
Papaver somniferum
phosphatidylserine + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + D-serine
for L- and D-serine a stereoselectivity of PLD is observed
694606
Brassica oleracea var. capitata
phosphatidylserine + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + ethanolamine
preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol
694606
Papaver somniferum
phosphatidylethanolamine + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + ethanolamine
preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-Ser
694606
Brassica oleracea var. capitata
phosphatidylethanolamine + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + ethanolamine
preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-serine
694606
Papaver somniferum
phosphatidylethanolamine + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + ethanolamine
preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-serine
694606
Brassica oleracea var. capitata
phosphatidylethanolamine + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + glycerol
preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol
694606
Papaver somniferum
phosphatidylglycerol + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + glycerol
preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-Ser
694606
Brassica oleracea var. capitata
phosphatidylglycerol + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + glycerol
preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-serine
694606
Papaver somniferum
phosphatidylglycerol + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + glycerol
preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-serine
694606
Brassica oleracea var. capitata
phosphatidylglycerol + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylcholine higher than phosphatidylglycerol equal to phosphatidylethanolamine higher than phosphatidylinositol higher than phosphatidylserine
694606
Brassica oleracea var. capitata
phosphatidate + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol
694606
Papaver somniferum
phosphatidate + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol equal to phosphatidylcholine higher than phosphatidylinositol higher than phosphatidylserine
694606
Brassica oleracea var. capitata
phosphatidate + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol higher than phosphatidylcholine higher than phosphatidylserine higher than phosphatidylinositol
694606
Papaver somniferum
phosphatidate + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + L-serine
for L- and D-serine a stereoselectivity of PLD is observed, preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-serine
694606
Papaver somniferum
phosphatidylserine + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + L-serine
for L- and D-serine a stereoselectivity of PLD is observed, preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-serine
694606
Brassica oleracea var. capitata
phosphatidylserine + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + L-serine
preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-Ser
694606
Brassica oleracea var. capitata
phosphatidylserine + choline
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylcholine higher than phosphatidylglycerol equal to phosphatidylethanolamine higher than phosphatidylinositol higher than phosphatidylserine
694606
Brassica oleracea var. capitata
phosphatidate + ethanolamine
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol
694606
Papaver somniferum
phosphatidate + ethanolamine
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol higher than phosphatidylserine
694606
Brassica oleracea var. capitata
phosphatidate + ethanolamine
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol higher than phosphatidylcholine higher than phosphatidylserine higher than phosphatidylinositol
694606
Papaver somniferum
phosphatidate + ethanolamine
-
-
-
?
3.1.4.4
phosphatidylglycerol + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylcholine higher than phosphatidylglycerol equal to phosphatidylethanolamine higher than phosphatidylinositol higher than phosphatidylserine
694606
Brassica oleracea var. capitata
phosphatidate + glycerol
-
-
-
?
3.1.4.4
phosphatidylglycerol + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol
694606
Papaver somniferum
phosphatidate + glycerol
-
-
-
?
3.1.4.4
phosphatidylglycerol + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol equal to phosphatidylcholine higher than phosphatidylinositol higher than phosphatidylserine
694606
Brassica oleracea var. capitata
phosphatidate + glycerol
-
-
-
?
3.1.4.4
phosphatidylglycerol + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol higher than phosphatidylcholine higher than phosphatidylserine higher than phosphatidylinositol
694606
Papaver somniferum
phosphatidate + glycerol
-
-
-
?
3.1.4.4
phosphatidylinositol + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylcholine higher than phosphatidylglycerol equal to phosphatidylethanolamine higher than phosphatidylinositol higher than phosphatidylserine
694606
Brassica oleracea var. capitata
phosphatidate + inositol
-
-
-
?
3.1.4.4
phosphatidylinositol + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol
694606
Papaver somniferum
phosphatidate + inositol
-
-
-
?
3.1.4.4
phosphatidylinositol + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol higher than phosphatidylserine
694606
Brassica oleracea var. capitata
phosphatidate + inositol
-
-
-
?
3.1.4.4
phosphatidylinositol + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol higher than phosphatidylcholine higher than phosphatidylserine higher than phosphatidylinositol
694606
Papaver somniferum
phosphatidate + inositol
-
-
-
?
3.1.4.4
phosphatidylserine + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylcholine higher than phosphatidylglycerol equal to phosphatidylethanolamine higher than phosphatidylinositol higher than phosphatidylserine
694606
Brassica oleracea var. capitata
phosphatidate + serine
-
-
-
?
3.1.4.4
phosphatidylserine + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol higher than phosphatidylserine
694606
Brassica oleracea var. capitata
phosphatidate + serine
-
-
-
?
3.1.4.4
phosphatidylserine + H2O
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol higher than phosphatidylcholine higher than phosphatidylserine higher than phosphatidylinositol
694606
Papaver somniferum
phosphatidate + serine
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.4.4
30
-
assay at
Brassica oleracea var. capitata
3.1.4.4
30
-
assay at
Papaver somniferum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
5.5
-
assay at
Brassica oleracea var. capitata
3.1.4.4
5.5
-
assay at
Papaver somniferum