BRENDA - Enzyme Database

Enantio-selectivity of human nucleoside monophosphate kinases

Alexandre, J.A.; Roy, B.; Topalis, D.; Perigaud, C.; Deville-Bonne, D.; Nucleosides Nucleotides Nucleic Acids 26, 1375-1379 (2007)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.14
0.02
-
D-CMP
pH 7.4, 37°C
Homo sapiens
2.7.4.14
0.05
-
D-UMP
pH 7.4, 37°C
Homo sapiens
2.7.4.14
0.36
-
2'-azido-D-dCMP
pH 7.4, 37°C
Homo sapiens
2.7.4.14
0.7
-
L-dUMP
pH 7.4, 37°C
Homo sapiens
2.7.4.14
0.73
-
L-dCMP
pH 7.4, 37°C
Homo sapiens
2.7.4.14
0.75
-
L-CMP
pH 7.4, 37°C
Homo sapiens
2.7.4.14
0.9
-
2'-azido-D-dUMP
pH 7.4, 37°C
Homo sapiens
2.7.4.14
1
-
D-dCMP
pH 7.4, 37°C
Homo sapiens
2.7.4.14
1.2
-
2'-azido-L-dCMP
pH 7.4, 37°C
Homo sapiens
2.7.4.14
1.3
-
2'-azido-L-dUMP
pH 7.4, 37°C
Homo sapiens
2.7.4.14
1.3
-
D-dUMP
pH 7.4, 37°C
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.4.14
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.4.14
ATP + CMP
Homo sapiens
-
ADP + CDP
-
-
?
2.7.4.14
ATP + dCMP
Homo sapiens
higher activity with the D-enantiomer compared to the L-enantiomer
ADP + dCDP
-
-
?
2.7.4.14
ATP + dUMP
Homo sapiens
higher activity with the D-enantiomer compared to the L-enantiomer
ADP + dUDP
-
-
?
2.7.4.14
ATP + UMP
Homo sapiens
-
ADP + UDP
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.4.14
Homo sapiens
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.14
ATP + 2'-azido-CMP
4fold higher activity with 2'-azido-dCMP than with 2'-azido-CMP
694479
Homo sapiens
ADP + 2'-azido-CDP
-
-
-
?
2.7.4.14
ATP + 2'-azido-dCMP
4fold higher activity with 2'-azido-dCMP than with 2'-azido-CMP
694479
Homo sapiens
ADP + 2'-azido-dCDP
-
-
-
?
2.7.4.14
ATP + 2'-azido-dUMP
similar activity with 2'-azido-UMP and 2'-azido-dUMP
694479
Homo sapiens
ADP + 2'-azido-dUDP
-
-
-
?
2.7.4.14
ATP + 2'-azido-UMP
similar activity with 2'-azido-UMP and 2'-azido-dUMP
694479
Homo sapiens
ADP + 2'-azido-UDP
-
-
-
?
2.7.4.14
ATP + CMP
-
694479
Homo sapiens
ADP + CDP
-
-
-
?
2.7.4.14
ATP + dCMP
higher activity with the D-enantiomer compared to the L-enantiomer
694479
Homo sapiens
ADP + dCDP
-
-
-
?
2.7.4.14
ATP + dUMP
higher activity with the D-enantiomer compared to the L-enantiomer
694479
Homo sapiens
ADP + dUDP
-
-
-
?
2.7.4.14
ATP + L-CMP
-
694479
Homo sapiens
ADP + L-CDP
-
-
-
-
2.7.4.14
ATP + L-dCMP
-
694479
Homo sapiens
ADP + L-dCDP
-
-
-
-
2.7.4.14
ATP + L-dUMP
-
694479
Homo sapiens
ADP + L-dUDP
-
-
-
-
2.7.4.14
ATP + UMP
-
694479
Homo sapiens
ADP + UDP
-
-
-
?
2.7.4.14
additional information
the enzyme shows enantioselectivity, acceptor-binding site structure of human UMP-CMP kinase in the closed form or in ligand-bound forms, overview
694479
Homo sapiens
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.4.14
37
-
assay at
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.4.14
7.4
-
assay at
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.4.14
ATP
-
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.4.14
ATP
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.14
0.02
-
D-CMP
pH 7.4, 37°C
Homo sapiens
2.7.4.14
0.05
-
D-UMP
pH 7.4, 37°C
Homo sapiens
2.7.4.14
0.36
-
2'-azido-D-dCMP
pH 7.4, 37°C
Homo sapiens
2.7.4.14
0.7
-
L-dUMP
pH 7.4, 37°C
Homo sapiens
2.7.4.14
0.73
-
L-dCMP
pH 7.4, 37°C
Homo sapiens
2.7.4.14
0.75
-
L-CMP
pH 7.4, 37°C
Homo sapiens
2.7.4.14
0.9
-
2'-azido-D-dUMP
pH 7.4, 37°C
Homo sapiens
2.7.4.14
1
-
D-dCMP
pH 7.4, 37°C
Homo sapiens
2.7.4.14
1.2
-
2'-azido-L-dCMP
pH 7.4, 37°C
Homo sapiens
2.7.4.14
1.3
-
2'-azido-L-dUMP
pH 7.4, 37°C
Homo sapiens
2.7.4.14
1.3
-
D-dUMP
pH 7.4, 37°C
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.4.14
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.4.14
ATP + CMP
Homo sapiens
-
ADP + CDP
-
-
?
2.7.4.14
ATP + dCMP
Homo sapiens
higher activity with the D-enantiomer compared to the L-enantiomer
ADP + dCDP
-
-
?
2.7.4.14
ATP + dUMP
Homo sapiens
higher activity with the D-enantiomer compared to the L-enantiomer
ADP + dUDP
-
-
?
2.7.4.14
ATP + UMP
Homo sapiens
-
ADP + UDP
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.14
ATP + 2'-azido-CMP
4fold higher activity with 2'-azido-dCMP than with 2'-azido-CMP
694479
Homo sapiens
ADP + 2'-azido-CDP
-
-
-
?
2.7.4.14
ATP + 2'-azido-dCMP
4fold higher activity with 2'-azido-dCMP than with 2'-azido-CMP
694479
Homo sapiens
ADP + 2'-azido-dCDP
-
-
-
?
2.7.4.14
ATP + 2'-azido-dUMP
similar activity with 2'-azido-UMP and 2'-azido-dUMP
694479
Homo sapiens
ADP + 2'-azido-dUDP
-
-
-
?
2.7.4.14
ATP + 2'-azido-UMP
similar activity with 2'-azido-UMP and 2'-azido-dUMP
694479
Homo sapiens
ADP + 2'-azido-UDP
-
-
-
?
2.7.4.14
ATP + CMP
-
694479
Homo sapiens
ADP + CDP
-
-
-
?
2.7.4.14
ATP + dCMP
higher activity with the D-enantiomer compared to the L-enantiomer
694479
Homo sapiens
ADP + dCDP
-
-
-
?
2.7.4.14
ATP + dUMP
higher activity with the D-enantiomer compared to the L-enantiomer
694479
Homo sapiens
ADP + dUDP
-
-
-
?
2.7.4.14
ATP + L-CMP
-
694479
Homo sapiens
ADP + L-CDP
-
-
-
-
2.7.4.14
ATP + L-dCMP
-
694479
Homo sapiens
ADP + L-dCDP
-
-
-
-
2.7.4.14
ATP + L-dUMP
-
694479
Homo sapiens
ADP + L-dUDP
-
-
-
-
2.7.4.14
ATP + UMP
-
694479
Homo sapiens
ADP + UDP
-
-
-
?
2.7.4.14
additional information
the enzyme shows enantioselectivity, acceptor-binding site structure of human UMP-CMP kinase in the closed form or in ligand-bound forms, overview
694479
Homo sapiens
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.4.14
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.4.14
7.4
-
assay at
Homo sapiens