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Literature summary extracted from

  • Sherrer, R.L.; O'Donoghue, P.; Söll D.
    Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation (2008), Nucleic Acids Res., 36, 1247-1259.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.164 expression in Escherichia coli BL21 Methanocaldococcus jannaschii

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.164 D41A strongly reduced activity Methanocaldococcus jannaschii
2.7.1.164 G14W strongly reduced activity Methanocaldococcus jannaschii
2.7.1.164 K17A strongly reduced activity Methanocaldococcus jannaschii
2.7.1.164 R116A mutant enzyme is 23.5fold less active than wild-type enzyme Methanocaldococcus jannaschii
2.7.1.164 R120A strongly reduced activity Methanocaldococcus jannaschii
2.7.1.164 S18A strongly reduced activity Methanocaldococcus jannaschii
2.7.1.164 T19W mutant enzyme is 2.8fold less active than wild-type enzyme Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.164 0.00004
-
 L-seryl-tRNASec 37°C Methanocaldococcus jannaschii
2.7.1.164 2.6
-
 ATP 37°C Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.164 ATP + L-seryl-tRNASec Methanocaldococcus jannaschii
-
 ADP + O-phospho-L-seryl-tRNASec
-
 ?
2.7.1.164 ATP + L-seryl-tRNASec Methanocaldococcus jannaschii DSM 2661
-
 ADP + O-phospho-L-seryl-tRNASec
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.164 Methanocaldococcus jannaschii Q58933 MJ1538
-
2.7.1.164 Methanocaldococcus jannaschii DSM 2661 Q58933 MJ1538
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.164
-
 Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.164 ATP + L-seryl-tRNASec
-
 Methanocaldococcus jannaschii ADP + O-phospho-L-seryl-tRNASec
-
 ?
2.7.1.164 ATP + L-seryl-tRNASec the enzyme does not recognize free Ser as a substrate for phosphate transfer. Ser attached to tRNASec is its obligate substrate. Neither tRNASer nor L-seryl-tRNASer is a substrate for phosphorylation Methanocaldococcus jannaschii ADP + O-phospho-L-seryl-tRNASec
-
 ?
2.7.1.164 ATP + L-seryl-tRNASec
-
 Methanocaldococcus jannaschii DSM 2661 ADP + O-phospho-L-seryl-tRNASec
-
 ?
2.7.1.164 ATP + L-seryl-tRNASec the enzyme does not recognize free Ser as a substrate for phosphate transfer. Ser attached to tRNASec is its obligate substrate. Neither tRNASer nor L-seryl-tRNASer is a substrate for phosphorylation Methanocaldococcus jannaschii DSM 2661 ADP + O-phospho-L-seryl-tRNASec
-
 ?
2.7.1.164 CTP + L-seryl-tRNASec phosphorylation at about 60% the activity of ATP Methanocaldococcus jannaschii CDP + O-phospho-L-seryl-tRNASec
-
 ?
2.7.1.164 CTP + L-seryl-tRNASec phosphorylation at about 60% the activity of ATP Methanocaldococcus jannaschii DSM 2661 CDP + O-phospho-L-seryl-tRNASec
-
 ?
2.7.1.164 dATP + L-seryl-tRNASec phosphorylation at about 65% the activity of ATP Methanocaldococcus jannaschii dADP + O-phospho-L-seryl-tRNASec
-
 ?
2.7.1.164 dATP + L-seryl-tRNASec phosphorylation at about 65% the activity of ATP Methanocaldococcus jannaschii DSM 2661 dADP + O-phospho-L-seryl-tRNASec
-
 ?
2.7.1.164 GTP + L-seryl-tRNASec phosphorylation at about 40% the activity of ATP Methanocaldococcus jannaschii GDP + O-phospho-L-seryl-tRNASec
-
 ?
2.7.1.164 ITP + L-seryl-tRNASec phosphorylation at about 85% the activity of ATP Methanocaldococcus jannaschii IDP + O-phospho-L-seryl-tRNASec
-
 ?
2.7.1.164 additional information no phosphorylation activity with 5'-adenylyl (beta,gamma-methylene)diphosphonate, very low phosphorylation activity with alpha,beta-methyleneadenosine 5'-triphosphate Methanocaldococcus jannaschii ?
-
 ?
2.7.1.164 additional information no phosphorylation activity with 5'-adenylyl (beta,gamma-methylene)diphosphonate, very low phosphorylation activity with alpha,beta-methyleneadenosine 5'-triphosphate Methanocaldococcus jannaschii DSM 2661 ?
-
 ?
2.7.1.164 UTP + L-seryl-tRNASec phosphorylation at about 40% the activity of ATP Methanocaldococcus jannaschii UDP + O-phospho-L-seryl-tRNASec
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.164 0.069
-
 ATP 37°C Methanocaldococcus jannaschii
2.7.1.164 0.098
-
 L-seryl-tRNASec 37°C Methanocaldococcus jannaschii