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Literature summary extracted from
- A CDC25 homologue from rice functions as an arsenate reductase (2007), New Phytol., 174, 311-321.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.20.99.1 | arsenate | - |
Oryza sativa |  |
| 1.20.99.1 | arsenate | dramatically increases expression of ACR2.1 | Oryza sativa | |
| 1.20.99.1 | additional information | ACR2.1 exhibits higher reductase activity than ACR2.2 | Oryza sativa | |
| 1.20.99.1 | additional information | ACR2.1 exhibits higher reductase activity than ACR2.2. Expression of ACR2.1 under conditions of phosphate starvation is faster than in the presence of phosphate | Oryza sativa |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.20.99.1 | expressed in an Escherichia coli strain in which the arsC gene is deleted and in a yeast (Saccharomyces cerevisiae) strain with a disrupted ACR2 gene | Oryza sativa |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.20.99.1 | C71S | mutagenesis of cysteine residues in the putative active site HC(X) 5 R motif leads to nearly complete loss of both phosphatase and arsenate reductase activities | Oryza sativa |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.20.99.1 | 0.1 | - |
reduced glutaredoxin | - |
Oryza sativa | |
| 1.20.99.1 | 0.2 | - |
reduced glutaredoxin | - |
Oryza sativa | |
| 1.20.99.1 | 9.2 | - |
arsenate | - |
Oryza sativa | |
| 1.20.99.1 | 9.9 | - |
arsenate | mutant C71S | Oryza sativa | |
| 1.20.99.1 | 12.2 | - |
arsenate | - |
Oryza sativa | |
| 1.20.99.1 | 24.4 | - |
arsenate | mutant C71S | Oryza sativa | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.20.99.1 | 14330 | - |
sequence analysis | Oryza sativa |
| 1.20.99.1 | 14960 | - |
sequence analysis | Oryza sativa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.20.99.1 | Oryza sativa | Q10SX6 | - |
- |
| 1.20.99.1 | Oryza sativa | Q336V5 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.20.99.1 | by nickel-nitrilotriacetic acid chromatography | Oryza sativa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.20.99.1 | root | ACR2.2 is observed only in roots following arsenate exposure | Oryza sativa | - |
| 1.20.99.1 | root | weak expression of ACR2.1 before addition of arsenate | Oryza sativa | - |
| 1.20.99.1 | shoot | - |
Oryza sativa | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.20.99.1 | arsenate + reduced glutaredoxin | yeast glutaredoxin ScGrx1p or Escherichia coli Grx2 | Oryza sativa | arsenite + acceptor | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.20.99.1 | ACR2.1 | - |
Oryza sativa |
| 1.20.99.1 | ACR2.2 | - |
Oryza sativa |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.20.99.1 | 0.03 | - |
arsenate | mutant C70S | Oryza sativa | |
| 1.20.99.1 | 0.05 | - |
arsenate | mutant C71S | Oryza sativa | |
| 1.20.99.1 | 0.14 | - |
arsenate | - |
Oryza sativa | |
| 1.20.99.1 | 0.19 | - |
reduced glutaredoxin | - |
Oryza sativa | |
| 1.20.99.1 | 0.26 | - |
reduced glutaredoxin | - |
Oryza sativa | |
| 1.20.99.1 | 0.33 | - |
arsenate | - |
Oryza sativa | |
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Release 2023.1 (January 2023)
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