Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Brueckner, F.; Cramer, P.
    Structural basis of transcription inhibition by alpha-amanitin and implications for RNA polymerase II translocation (2008), Nat. Struct. Mol. Biol., 15, 811-818.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.6 in complex with alpha-amanitin, hanging drop vapour diffusion method, with 200 mM ammonium acetate, 300 mM sodium acetate, 50 mM HEPES, pH 7.0, 4-7% (w/v) PEG 6000 and 5 mM Tris(2-carboxyethyl) phosphine Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.6 alpha-Amanitin alpha-amanitin inhibits Pol II by trapping the wedged trigger loop and shifted bridge helix, thereby stabilizing a conformation of the elongation complex that apparently represents a translocation intermediate Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.6 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.6 chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.6 nucleoside triphosphate + RNAn
-
 Saccharomyces cerevisiae diphosphate + RNAn+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.6 Pol II
-
 Saccharomyces cerevisiae
2.7.7.6 RNA polymerase II
-
 Saccharomyces cerevisiae