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Literature summary extracted from

  • Lin, C.C.; Yin, M.C.
    Antiglycative and anti-VEGF effects of s-ethyl cysteine and s-propyl cysteine in kidney of diabetic mice (2008), Mol. Nutr. Food Res., 52, 1358-1364.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.4.1.5 ascorbic acid enhances slightly glyoxalase I activity, dose-dependently 0-2 g/l, pH 6.6, 37°C Mus musculus
4.4.1.5 S-ethyl cysteine enhances significantly glyoxalase I activity, dose-dependently 0-2 g/l, pH 6.6, 37°C Mus musculus
4.4.1.5 S-propyl-cysteine enhances significantly glyoxalase I activity, dose-dependently 0-2 g/l, pH 6.6, 37°C Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.5 Mus musculus
-
 male Balb/cA, 3-4 week old
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.4.1.5 kidney male Balb/cA mice, 3-4 week old Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.5 0.172 180 without addition of S-ethyl cysteine, s-propyl-cysteine, or ascorbic acid, pH 6.6, 37°C Mus musculus
4.4.1.5 0.184
-
 slightly increased activity compared to enzyme assay without ascorbic acid, pH 6.6, 37°C Mus musculus
4.4.1.5 0.19
-
 slightly increased activity compared to enzyme assay without ascorbic acid, pH 6.6, 37°C Mus musculus
4.4.1.5 0.218
-
 in the presence of 1 g/l S-ethyl cysteine, 1.2fold higher activity than without S-ethyl cysteine, pH 6.6, 37°C Mus musculus
4.4.1.5 0.221
-
 in the presence of 1 g/l S-propyl-cysteine, 1.3fold higher activity than without S-ethyl cysteine, pH 6.6, 37°C Mus musculus
4.4.1.5 0.247
-
 in the presence of 2 g/l S-ethyl cysteine, 1.4fold higher activity than without S-ethyl cysteine, pH 6.6, 37°C Mus musculus
4.4.1.5 0.258
-
 in the presence of 2 g/l S-propyl-cysteine, 1.5fold higher activity than without S-ethyl cysteine, pH 6.6, 37°C Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.5 methylglyoxal + glutathione
-
 Mus musculus S-((R)-lactoyl)glutathione product measured by monitoring the increase of absorbance at 240 nm by formation of S-D-lactoylglutathione, pH 6.6, 37°C ?

Synonyms

EC Number Synonyms Comment Organism
4.4.1.5 glyoxalase I
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.4.1.5 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.5 6.6
-
 assay at Mus musculus