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Literature summary extracted from

  • Foerster-Fromme, K.; Chattopadhyay, A.; Jendrossek, D.
    Biochemical characterization of AtuD from Pseudomonas aeruginosa, the first member of a new subgroup of acyl-CoA dehydrogenases with specificity for citronellyl-CoA (2008), Microbiology, 154, 789-796.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.99.B2 expression in Escherichia coli Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
1.3.99.B2 additional information gene insertion mutant is not able to grow on citronellol, geraniol, citronellate, geranylate as sole carbon source Pseudomonas aeruginosa
1.3.99.B2 additional information gene insertion mutant mutants grow slightly more slowly on citronellol and citronellate but show normal growth on geraniol and geranylate Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.99.B2 0.0016
-
 citronellyl-CoA pH 7.0, 30°C Pseudomonas aeruginosa
1.3.99.B2 0.018
-
 citronellyl-CoA pH 7.0, 30°C Pseudomonas aeruginosa
1.3.99.B2 0.13
-
 octanoyl-CoA pH 7.0, 30°C Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.99.B2 41000
-
 x * 41000, SDS-PAGE, x * 42000, calculated Pseudomonas aeruginosa
1.3.99.B2 41000
-
 x * 41000, SDS-PAGE, x * 42700, calculated Pseudomonas aeruginosa
1.3.99.B2 42000
-
 x * 41000, SDS-PAGE, x * 42000, calculated Pseudomonas aeruginosa
1.3.99.B2 42700
-
 x * 41000, SDS-PAGE, x * 42700, calculated Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.B2 Pseudomonas aeruginosa Q9HZV8 gene atud; specific expression in cells growing on acyclic terpenes, absent in isovalerate- or succinate-grown cells
-
1.3.99.B2 Pseudomonas aeruginosa Q9I3H8 gene PA1535, second citronellyl-CoA dehydrogenase, less specific; specific expression in cells growing on acyclic terpenes, absent in isovalerate- or succinate-grown cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.99.B2 recombinant protein Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.B2 citronellyl-CoA + 2,6-dichlorophenolindophenol
-
 Pseudomonas aeruginosa cis-geranyl-CoA + reduced 2,6-dichlorophenolindophenol
-
 ?
1.3.99.B2 additional information no substrates: 5-methylhex-4-enoyl-CoA or isovaleryl-CoA Pseudomonas aeruginosa ?
-
 ?
1.3.99.B2 additional information no substrates: octanoyl-CoA, 5-methylhex-4-enoyl-CoA or isovaleryl-CoA Pseudomonas aeruginosa ?
-
 ?
1.3.99.B2 octanoyl-CoA + 2,6-dichlorophenolindophenol
-
 Pseudomonas aeruginosa cis-geranyl-CoA + reduced 2,6-dichlorophenolindophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.99.B2 ? x * 41000, SDS-PAGE, x * 42000, calculated Pseudomonas aeruginosa
1.3.99.B2 ? x * 41000, SDS-PAGE, x * 42700, calculated Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.99.B2 FAD purified and concentrated protein is yellow, and spectral analysis confirms the presence of FAD as cofactor Pseudomonas aeruginosa