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Literature summary extracted from
- The PA4204 gene encodes a periplasmic gluconolactonase (PpgL) which is important for fitness of Pseudomonas aeruginosa (2008), Microbiology, 154, 2979-2990.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.17 | expressed in Escherichia coli | Pseudomonas aeruginosa |
| 3.1.1.31 | expressed as a His fusion protein in Escherichia coli BL21(DE3) cells | Pseudomonas aeruginosa |
| 5.5.1.2 | expressed in Escherichia coli BL21(DE3) cells | Pseudomonas aeruginosa |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.17 | EDTA | partially sensitive to EDTA | Pseudomonas aeruginosa |  |
| 5.5.1.2 | EDTA | the enzyme is partially sensitive to EDTA | Pseudomonas aeruginosa | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.1.17 | periplasm | - |
Pseudomonas aeruginosa | - |
- |
| 3.1.1.31 | periplasm | - |
Pseudomonas aeruginosa | - |
- |
| 5.5.1.2 | periplasm | - |
Pseudomonas aeruginosa | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.17 | 40000 | - |
about 40000 Da, SDS-PAGE | Pseudomonas aeruginosa |
| 3.1.1.31 | 40000 | - |
SDS-PAGE | Pseudomonas aeruginosa |
| 5.5.1.2 | 40000 | - |
SDS-PAGE | Pseudomonas aeruginosa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.17 | Pseudomonas aeruginosa | - |
strains PAO-S1 and PAO-B1 | - |
| 3.1.1.31 | Pseudomonas aeruginosa | - |
strains PAO-S1 and PAO-B1 | - |
| 5.5.1.2 | Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.17 | His-Trap nickel affinity chromatography column chromatography and Superdex 75 gel filtration | Pseudomonas aeruginosa |
| 3.1.1.31 | His-Trap nickel affinity column chromatography and Superdex 75 gel filtration | Pseudomonas aeruginosa |
| 5.5.1.2 | His-Trap nickel affinity column chromatography | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.17 | (S)-5-oxo-2-tetrahydrofurancarboxylic acid + H2O | - |
Pseudomonas aeruginosa | L-alpha-hydroxyglutaric acid | - |
? | |
| 3.1.1.17 | D-glucono-1,5-lactone + H2O | - |
Pseudomonas aeruginosa | D-gluconate | - |
? | |
| 3.1.1.31 | (S)-5-oxo-2-tetrahydrofurancarboxylic acid + H2O | - |
Pseudomonas aeruginosa | L-alpha-hydroxyglutaric acid | - |
? | |
| 3.1.1.31 | 6-phospho-D-glucono-1,5-lactone + H2O | - |
Pseudomonas aeruginosa | 6-phospho-D-gluconate | - |
? | |
| 3.1.1.31 | D-glucono-delta-lactone + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| 5.5.1.2 | (S)-5-oxo-2-tetrahydrofurancarboxylic acid | - |
Pseudomonas aeruginosa | L-alpha-hydroxyglutaric acid | - |
? | |
| 5.5.1.2 | 2-Carboxy-2,5-dihydro-5-oxofuran-2-acetate | - |
Pseudomonas aeruginosa | cis-Butadiene-1,2,4-tricarboxylate | - |
? | |
| 5.5.1.2 | D-glucono-D-lactone | - |
Pseudomonas aeruginosa | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.17 | PpgL | - |
Pseudomonas aeruginosa |
| 3.1.1.31 | PGL | - |
Pseudomonas aeruginosa |
| 5.5.1.2 | 3-Carboxy-cis,cis-muconate lactonizing enzyme | - |
Pseudomonas aeruginosa |
| 5.5.1.2 | CMLE | - |
Pseudomonas aeruginosa |
| 5.5.1.2 | PA4204 protein | - |
Pseudomonas aeruginosa |
| 5.5.1.2 | PpgL | - |
Pseudomonas aeruginosa |
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