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Literature summary extracted from

  • Katsuyama, Y.; Matsuzawa, M.; Funa, N.; Horinouchi, S.
    Production of curcuminoids by Escherichia coli carrying an artificial biosynthesis pathway (2008), Microbiology, 154, 2620-2628.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.12 expressed in Escherichia coli strains JM109 and BLR(DE3) Lithospermum erythrorhizon

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.211 additional information construction of an artificial curcuminoid biosynthetic pathway, including reactions of phenylalanine ammonia-lyase from the yeast Rhodotorula rubra, 4-coumarate:CoA ligase from Lithospermum erythrorhizon and curcuminoid synthase from Oryza sativa in Escherichia coli for the production of curcuminoids. Cultivation of the recombinant Escherichia coli cells in the presence of tyrosine or phenylalanine, or both, leads to production of bisdemethoxycurcumin, dicinnamoylmethane and cinnamoyl-4-coumaroylmethane, overview. Another Escherichia coli system carrying the 4-coumarate:CoA ligase and the CUS genes is also useful for high-yield production of curcuminoids from exogenously supplemented phenylpropanoid acids 4-coumaric acid, cinnamic acid and ferulic acid Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.211 2 4-coumaroyl-CoA + malonyl-CoA + H2O Oryza sativa
-
 3 CoA + bisdemethoxycurcumin + 2 CO2
-
 ?
2.3.1.211 2 cinnamoyl-CoA + malonyl-CoA + H2O Oryza sativa
-
 3 CoA + dicinnamoylmethane + 2 CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.211 Oryza sativa
-
-
-
6.2.1.12 Lithospermum erythrorhizon
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.211 2 4-coumaroyl-CoA + malonyl-CoA + H2O = 3 CoA + bisdemethoxycurcumin + 2 CO2 the reaction proceeds in three steps via formation of 4-coumaroyldiketide-CoA and 4-coumaroyl beta-keto acid, overview Oryza sativa
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.211 2 4-coumaroyl-CoA + malonyl-CoA + H2O
-
 Oryza sativa 3 CoA + bisdemethoxycurcumin + 2 CO2
-
 ?
2.3.1.211 2 cinnamoyl-CoA + malonyl-CoA + H2O
-
 Oryza sativa 3 CoA + dicinnamoylmethane + 2 CO2
-
 ?
2.3.1.211 4-coumaroyl-CoA + cinnamoyl-CoA + malonyl-CoA + H2O
-
 Oryza sativa 3 CoA + cinnamoyl-4-coumaroyl-methane + 2 CO2
-
 ?
6.2.1.12 ATP + (2E)-3-(4-hydroxy-3-methoxyphenyl)prop-2-enoic acid + CoA
-
 Lithospermum erythrorhizon ?
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA
-
 Lithospermum erythrorhizon AMP + diphosphate + 4-coumaroyl-CoA
-
 ir
6.2.1.12 ATP + cinnamate + CoA
-
 Lithospermum erythrorhizon AMP + diphosphate + cinnamoyl-CoA
-
 ir

Synonyms

EC Number Synonyms Comment Organism
2.3.1.211 curcuminoid synthase
-
 Oryza sativa
2.3.1.211 CUS
-
 Oryza sativa
6.2.1.12 4-coumarate: CoA ligase
-
 Lithospermum erythrorhizon
6.2.1.12 4CL
-
 Lithospermum erythrorhizon

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.211 26
-
 in vivo assay at Oryza sativa

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.12 ATP
-
 Lithospermum erythrorhizon