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Literature summary extracted from
- tRNA-dependent asparagine formation in prokaryotes: Characterization, isolation and structural and functional analysis of a ribonucleoprotein particle generating Asn-tRNAAsn (2008), Methods, 44, 146-163.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 6.1.1.12 | gene aspS2, expression in Escherichia coli strain JM103 | Thermus thermophilus |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 6.1.1.12 | AspRS2, hanging drop vapour diffusion method, 0.002 ml of 14 mg/ml protein with 10% m/v PEG 8000, 200 mM NaCl, and 100 mM Na-CHES buffer, pH 9.5, X-ray diffraction structure determination and analysis at 2.3-3.2 A resolution | Thermus thermophilus |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.1.1.12 | Mg2+ | - |
Thermus thermophilus |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.1.1.12 | ATP + L-aspartate + tRNAAsn | Thermus thermophilus | non-discriminating AspRS2 | AMP + diphosphate + L-aspartyl-tRNAAsn | - |
? | |
| 6.1.1.12 | ATP + L-aspartate + tRNAAsn | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | non-discriminating AspRS2 | AMP + diphosphate + L-aspartyl-tRNAAsn | - |
? | |
| 6.1.1.12 | ATP + L-aspartate + tRNAAsp | Thermus thermophilus | - |
AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
| 6.1.1.12 | ATP + L-aspartate + tRNAAsp | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
| 6.1.1.12 | additional information | Thermus thermophilus | in Thermus thermophilus Asn-tRNAAsn is formed indirectly via a two-step pathway whereby tRNAAsn is mischarged with Asp that will subsequently be amidated into Asn by an amidotransferase.The non-discriminating aspartyl-tRNA synthetase, the trimeric GatCAB tRNA-dependent amidotransferase and the tRNAAsn promoting this pathway assemble into a ribonucleoprotein particle termed transamidosome, analysis of the mechanism of Asn-tRNAAsn formation by the transamidosome, overview | ? | - |
? | |
| 6.1.1.12 | additional information | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | in Thermus thermophilus Asn-tRNAAsn is formed indirectly via a two-step pathway whereby tRNAAsn is mischarged with Asp that will subsequently be amidated into Asn by an amidotransferase.The non-discriminating aspartyl-tRNA synthetase, the trimeric GatCAB tRNA-dependent amidotransferase and the tRNAAsn promoting this pathway assemble into a ribonucleoprotein particle termed transamidosome, analysis of the mechanism of Asn-tRNAAsn formation by the transamidosome, overview | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 6.1.1.12 | Thermus thermophilus | - |
gene aspS2 | - |
| 6.1.1.12 | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
gene aspS2 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 6.1.1.12 | recombinant AspRS2 from Escherichia coli by two different steps of anion exchange chromatography and hydroxy apatite chromatography | Thermus thermophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.1.1.12 | ATP + L-aspartate + tRNAAsn | non-discriminating AspRS2 | Thermus thermophilus | AMP + diphosphate + L-aspartyl-tRNAAsn | - |
? | |
| 6.1.1.12 | ATP + L-aspartate + tRNAAsn | non-discriminating AspRS2, recombinantly produced tRNAAsn | Thermus thermophilus | AMP + diphosphate + L-aspartyl-tRNAAsn | - |
? | |
| 6.1.1.12 | ATP + L-aspartate + tRNAAsn | non-discriminating AspRS2 | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-aspartyl-tRNAAsn | - |
? | |
| 6.1.1.12 | ATP + L-aspartate + tRNAAsp | - |
Thermus thermophilus | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
| 6.1.1.12 | ATP + L-aspartate + tRNAAsp | discriminating AspRS1 and non-discriminating AspRS2 | Thermus thermophilus | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
| 6.1.1.12 | ATP + L-aspartate + tRNAAsp | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
| 6.1.1.12 | ATP + L-aspartate + tRNAAsp | discriminating AspRS1 and non-discriminating AspRS2 | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
| 6.1.1.12 | additional information | in Thermus thermophilus Asn-tRNAAsn is formed indirectly via a two-step pathway whereby tRNAAsn is mischarged with Asp that will subsequently be amidated into Asn by an amidotransferase.The non-discriminating aspartyl-tRNA synthetase, the trimeric GatCAB tRNA-dependent amidotransferase and the tRNAAsn promoting this pathway assemble into a ribonucleoprotein particle termed transamidosome, analysis of the mechanism of Asn-tRNAAsn formation by the transamidosome, overview | Thermus thermophilus | ? | - |
? | |
| 6.1.1.12 | additional information | Thermus thermophilus contains two AspRSs: the discriminating AspRS1 which aspartylates only tRNAAsp and the non-discriminating AspRS2 which aspartylates tRNAAsn as efficiently as tRNAAsp | Thermus thermophilus | ? | - |
? | |
| 6.1.1.12 | additional information | in Thermus thermophilus Asn-tRNAAsn is formed indirectly via a two-step pathway whereby tRNAAsn is mischarged with Asp that will subsequently be amidated into Asn by an amidotransferase.The non-discriminating aspartyl-tRNA synthetase, the trimeric GatCAB tRNA-dependent amidotransferase and the tRNAAsn promoting this pathway assemble into a ribonucleoprotein particle termed transamidosome, analysis of the mechanism of Asn-tRNAAsn formation by the transamidosome, overview | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ? | - |
? | |
| 6.1.1.12 | additional information | Thermus thermophilus contains two AspRSs: the discriminating AspRS1 which aspartylates only tRNAAsp and the non-discriminating AspRS2 which aspartylates tRNAAsn as efficiently as tRNAAsp | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 6.1.1.12 | non-discriminating aspartyl-tRNA synthetase | - |
Thermus thermophilus |
| 6.1.1.12 | non-discriminating AspRS | - |
Thermus thermophilus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 6.1.1.12 | 37 | 50 | assay at 37°C or 50°C | Thermus thermophilus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 6.1.1.12 | 7.2 | - |
assay at | Thermus thermophilus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.1.1.12 | ATP | - |
Thermus thermophilus | |
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