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Literature summary extracted from

  • Engels, B.; Dahm, P.; Jennewein, S.
    Metabolic engineering of taxadiene biosynthesis in yeast as a first step towards Taxol (Paclitaxel) production (2008), Metab. Eng., 10, 201-206.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.3.17 additional information coexpression of geranylgeranyl diphosphate synthase gene from Sulfolobus acidocaldarius and codon optimzing of the Taxus chinensis taxadiene synthase gene increases the production of taxadiene in Saccharomyces cerevisiae Taxus chinensis

Application

EC Number Application Comment Organism
4.2.3.17 drug development cancer therapy, taxadiene is the precursor of paclitaxel, a chemotherapeutic agents Taxus chinensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.17 Expression of Taxus chinensis taxadiene synthase (expression of a transgene encoding the pseudomature form which lacks the N-terminal 74-amino acid residue plastid-targeting sequence) in Saccharomyces cerevisiae alone does not increase taxadiene levels because of insufficient levels of the universal diterpenoid precursor geranylgeranyl diphosphate. Coexpression of Taxus chinensis taxadiene synthase and geranylgeranyl diphosphate synthase fails to increase levels. Replacement the Taxus chinensis geranylgeranyl diphosphate synthase with its counterpart from Sulfolobus acidocaldarius, which does not compete with steroid synthesis, and codon optimized the Taxus chinensis taxadiene synthase gene to ensure high-level expression, resulting in a increase in taxadiene as well as significant amounts of geranylgeraniol, suggesting taxadiene levels can be increased even further. Taxus chinensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.17 geranylgeranyl diphosphate Taxus chinensis taxadiene biosynthetic pathway taxa-4(5),11(12)-diene + diphosphate precursor of the chemotherapeutic agent taxol ir

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.17 Taxus chinensis
-
 production of enzyme in Saccharomyces cerevisiae
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.17 cell culture
-
 Taxus chinensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.17 additional information
-
 Replacement of the geranylgeranyl diphosphate synthase with the enzyme from Sulfolobus acidocaldarius and codon optimizing of the Taxus chinensis taxadiene synthase gene to ensure high-level expression, resulting in a 40fold increase in taxadiene to 8.770.85 mg/l as well as significant amounts of geranylgeraniol (33.1 mg/l) Taxus chinensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.17 geranylgeranyl diphosphate taxadiene biosynthetic pathway Taxus chinensis taxa-4(5),11(12)-diene + diphosphate precursor of the chemotherapeutic agent taxol ir

Synonyms

EC Number Synonyms Comment Organism
4.2.3.17 taxadiene synthase
-
 Taxus chinensis