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Literature summary extracted from
- Molecular evolution of a steroid hydroxylating cytochrome P450 using a versatile steroid detection system for screening (2008), Lipids, 43, 1133-1141.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.13.54 | expression in Escherichia coli | Priestia megaterium |
| 1.14.15.8 | CYP106A2 can be easily expressed in Escherichia coli with a high yield and can be reconstituted using the adrenal redox proteins, adrenodoxin and adrenodoxin reductase | Priestia megaterium |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.14.15.8 | A106T | kcat/Km for conversion of 11-deoxycortisol is 3.17fold higher than wild-type value, 1.43fold increase in progesterone conversion | Priestia megaterium |
| 1.14.15.8 | A106T/Q189K/T399S/R409L | kcat/Km for conversion of 11-deoxycortisol is 4.3fold higher than wild-type value, 1.13fold increase in progesterone conversion | Priestia megaterium |
| 1.14.15.8 | A106T/R409L | kcat/Km for conversion of 11-deoxycortisol is 3fold higher than wild-type value, 1.43fold increase in progesterone conversion | Priestia megaterium |
| 1.14.15.8 | D153V/I214F | kcat/Km for conversion of 11-deoxycortisol is 2.64fold higher than wild-type value, progesterone conversion is 93% of wild-type value | Priestia megaterium |
| 1.14.15.8 | D217V | kcat/Km for conversion of 11-deoxycortisol is 2.68fold higher than wild-type value, 1.18fold increase in progesterone conversion | Priestia megaterium |
| 1.14.15.8 | F165I | 1.25fold increase in progesterone conversion | Priestia megaterium |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.15.8 | 0.037 | - |
11-deoxycortisol | pH 7.4, 30°C, mutant A106T/Q189K/T399S/R409L, mutant D153V/I214F | Priestia megaterium |  |
| 1.14.15.8 | 0.059 | - |
11-deoxycortisol | pH 7.4, 30°C, mutant A106T | Priestia megaterium | |
| 1.14.15.8 | 0.066 | - |
11-deoxycortisol | pH 7.4, 30°C, mutant D217V | Priestia megaterium | |
| 1.14.15.8 | 0.068 | - |
11-deoxycortisol | pH 7.4, 30°C, mutant A106T/R409L | Priestia megaterium | |
| 1.14.15.8 | 0.093 | - |
11-deoxycortisol | pH 7.4, 30°C, CYP106A2 wild-type enzyme | Priestia megaterium | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.54 | Priestia megaterium | - |
strain ATCC 13368 | - |
| 1.14.15.8 | Priestia megaterium | Q06069 | ATCC 13368 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.15.8 | - |
Priestia megaterium |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 1.14.13.54 | reconstitution of recombinant protein using adrenodoxin and adrenodoxin reductase | Priestia megaterium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.54 | 11-deoxycortisol + NADPH + O2 | - |
Priestia megaterium | ? + NADP+ + H2O | - |
? | |
| 1.14.13.54 | progesterone + NADPH + H+ + O2 | - |
Priestia megaterium | Testosterone acetate + NADP+ + H2O | - |
? | |
| 1.14.15.8 | 11-deoxycortisol + reduced adrenodoxin + O2 | adrenodoxin is a [2Fe-2S] ferredoxin involved in electron transfer from NADPH+ (via a reductase) to cytochrome P-450 in the adrenal gland | Priestia megaterium | 15beta-15,17,21-trihydroxypregn-4-ene-3,20-dione + oxidized adrenodoxin + H2O | - |
? | |
| 1.14.15.8 | progesterone + reduced adrenodoxin + O2 | adrenodoxin is a [2Fe-2S] ferredoxin involved in electron transfer from NADPH+ (via a reductase) to cytochrome P-450 in the adrenal gland | Priestia megaterium | 15beta-hydroxyprogesterone + oxidized adrenodoxin + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.13.54 | CYP106A2 | - |
Priestia megaterium |
| 1.14.13.54 | steroid hydroxylating cytochrome | - |
Priestia megaterium |
| 1.14.15.8 | CYP106A2 | - |
Priestia megaterium |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.8 | 30 | - |
assay at | Priestia megaterium |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.8 | 7.4 | - |
assay at | Priestia megaterium |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.15.8 | adrenodoxin | - |
Priestia megaterium | |
| 1.14.15.8 | NADPH | - |
Priestia megaterium | |
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