Literature summary extracted from
Songsiriritthigul, C.; Pantoom, S.; Aguda, A.H.; Robinson, R.C.; Suginta, W.
Crystal structures of Vibrio harveyi chitinase A complexed with chitooligosaccharides: implications for the catalytic mechanism (2008), J. Struct. Biol., 162, 491-499.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.2.1.14 |
expression of C-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli |
Vibrio harveyi |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
3.2.1.14 |
purified recombinant His6-tagged wild-type and mutant E315M mutant enzymes, free or bound to (GlcNAC)5 or (GlcNAc)6, sitting drop vapour diffusion method, 20 nl of protein solution containing 20 mg/ml wild-type protein in 10 mM Tris-HCl, pH 8.0, are mixed with 20 nl of precipitant solution, macroseeding, or 5 mg/ml protein in 1.2 M ammonium sulfate and 0.1 M Tris-HC, pH 8.0, at 14°C for 2 days, mutant E315M crystals from 20% w/v PEG 4000, 0.1 M ammonium sulfate and 0.1 M Tris-HCl, pH 7.5, 1 week at 14°C, X-ray diffraction structure determination and analysis at 1.7-2.0 A resolution |
Vibrio harveyi |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.2.1.14 |
E315M |
a catalytically inactive mutant, the binding cleft of the ligand-free mutant is partially blocked by the C-terminal His6-tag, structures of E315M-chitooligosaccharide complexes, overview |
Vibrio harveyi |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.2.1.14 |
Vibrio harveyi |
- |
- |
- |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
3.2.1.14 |
chitopentaose + H2O = chitobiose + chitotriose |
chitooligosaccharide-binding modes and catalytic mechanism, overview |
Vibrio harveyi |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.2.1.14 |
More |
three distinct domains: 1. the amino-terminal chitin-binding domain, 2. the main catalytic (alpha/beta)8 TIM-barrel domain, and 3. the small (alpha + beta) insertion domain. The catalytic cleft of chitinase A has a long, deep groove, which contains six chitooligosaccharide ring-binding subsites, overview |
Vibrio harveyi |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.2.1.14 |
chitinase A |
- |
Vibrio harveyi |
3.2.1.14 |
More |
chitinase A belongs to the glycosyl hydrolase family 18 |
Vibrio harveyi |