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Literature summary extracted from

  • Zhu, X.; De Laurentis, W.; Leang, K.; Herrmann, J.; Ihlefeld, K.; van Pee, K.H.; Naismith, J.H.
    Structural insights into regioselectivity in the enzymatic chlorination of tryptophan (2009), J. Mol. Biol., 391, 74-85.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.58 His-tagged apoPyrH was expressed in Pseudomonas fluorescens BL915 Streptomyces rugosporus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.19.58 sitting-drop vapor-diffusion method at 20°C, crystal structure of a tryptophan 5-halogenase (PyrH) bound to tryptophan and FAD Streptomyces rugosporus

Protein Variants

EC Number Protein Variants Comment Organism
1.14.19.58 E46D mutant enzyme folds properly Streptomyces rugosporus
1.14.19.58 E46Q mutant enzyme folds properly Streptomyces rugosporus
1.14.19.58 F49A/Y454F mutant enzyme is incorrectly folded Streptomyces rugosporus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.19.58 L-tryptophan substrate inhibition above 0.06 mM Streptomyces rugosporus

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.58 Streptomyces rugosporus A4D0H5
-
-
1.14.19.58 Streptomyces rugosporus LL-42D005 A4D0H5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.19.58
-
 Streptomyces rugosporus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.58 L-tryptophan + FADH2 + Cl- + O2 + H+
-
 Streptomyces rugosporus 5-chloro-L-tryptophan + FAD + 2 H2O
-
 ?
1.14.19.58 L-tryptophan + FADH2 + Cl- + O2 + H+
-
 Streptomyces rugosporus LL-42D005 5-chloro-L-tryptophan + FAD + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.58 pyrH
-
 Streptomyces rugosporus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.19.58 30
-
 assay at Streptomyces rugosporus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.19.58 7.2
-
 assay at Streptomyces rugosporus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.19.58 0.01
-
 L-tryptophan mutant enzyme E46D, in potassium phosphate buffer (10 mM, pH 7.2), at 30°C Streptomyces rugosporus
1.14.19.58 0.027
-
 L-tryptophan mutant enzyme F49A, in potassium phosphate buffer (10 mM, pH 7.2), at 30°C Streptomyces rugosporus
1.14.19.58 0.039
-
 L-tryptophan mutant enzyme E46Q, in potassium phosphate buffer (10 mM, pH 7.2), at 30°C Streptomyces rugosporus
1.14.19.58 0.05
-
 L-tryptophan mutant enzyme Y454F, in potassium phosphate buffer (10 mM, pH 7.2), at 30°C Streptomyces rugosporus
1.14.19.58 0.54
-
 L-tryptophan wild type enzyme, in potassium phosphate buffer (10 mM, pH 7.2), at 30°C Streptomyces rugosporus