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Literature summary extracted from

  • Nakamura, T.; Mine, S.; Hagihara, Y.; Ishikawa, K.; Ikegami, T.; Uegaki, K.
    Tertiary structure and carbohydrate recognition by the chitin-binding domain of a hyperthermophilic chitinase from Pyrococcus furiosus (2008), J. Mol. Biol., 381, 670-680.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.14 wild-type and mutant ChBD2 domains, X-ray diffraction structure determination and analysis at 1.7-1.8 A resolution Pyrococcus furiosus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.14 E279A site-directed mutagenesis of the ChBD2 domain residue, the mutant shows altered substrate binding affinity compared to the wild-type enzyme domain Pyrococcus furiosus
3.2.1.14 E279T/D281N site-directed mutagenesis of the ChBD2 domain residues, the mutant shows altered substrate binding affinity and specificity compared to the wild-type enzyme domain Pyrococcus furiosus
3.2.1.14 I337A site-directed mutagenesis of the ChBD2 domain residue, the mutant shows altered substrate binding affinity compared to the wild-type enzyme domain Pyrococcus furiosus
3.2.1.14 N272A site-directed mutagenesis of the ChBD2 domain residue, the mutant shows altered substrate binding affinity compared to the wild-type enzyme domain Pyrococcus furiosus
3.2.1.14 N327A site-directed mutagenesis of the ChBD2 domain residue, the mutant shows altered substrate binding affinity compared to the wild-type enzyme domain Pyrococcus furiosus
3.2.1.14 N339A site-directed mutagenesis of the ChBD2 domain residue, the mutant shows altered substrate binding affinity compared to the wild-type enzyme domain Pyrococcus furiosus
3.2.1.14 S306A site-directed mutagenesis of the ChBD2 domain residue, the mutant shows altered substrate binding affinity compared to the wild-type enzyme domain Pyrococcus furiosus
3.2.1.14 S309A site-directed mutagenesis of the ChBD2 domain residue, the mutant shows altered substrate binding affinity compared to the wild-type enzyme domain Pyrococcus furiosus
3.2.1.14 T283A site-directed mutagenesis of the ChBD2 domain residue, the mutant shows altered substrate binding affinity compared to the wild-type enzyme domain Pyrococcus furiosus
3.2.1.14 W274A site-directed mutagenesis of the ChBD2 domain residue, the mutant shows altered substrate binding affinity compared to the wild-type enzyme domain Pyrococcus furiosus
3.2.1.14 W308A site-directed mutagenesis of the ChBD2 domain residue, the mutant shows altered substrate binding affinity compared to the wild-type enzyme domain Pyrococcus furiosus
3.2.1.14 W326A site-directed mutagenesis of the ChBD2 domain residue, the mutant shows altered substrate binding affinity compared to the wild-type enzyme domain Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.14 chitin + H2O Pyrococcus furiosus the chitinase is a hyperthermophilic glycosidase that effectively hydrolyzes both alpha and beta crystalline chitins, carbohydrate recognition by the chitin-binding domain and substrate binding structure, overview ?
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 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Pyrococcus furiosus
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O the chitinase is a hyperthermophilic glycosidase that effectively hydrolyzes both alpha and beta crystalline chitins, carbohydrate recognition by the chitin-binding domain and substrate binding structure, overview Pyrococcus furiosus ?
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 ?
3.2.1.14 chitin + H2O the surface resiudes Trp274, Trp308 and Trp326 flanked by acidic residues Glu279 and Asp281 are involved in chitin binding Pyrococcus furiosus ?
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 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 More the chitinase has unique structural features and contains two catalytic domains AD1 and AD2 and two chitin-binding domains ChBD1 and ChBD2. ChBD2 consists of two four-stranded beta-sheets and is composed of a typical beta-sandwich architecture, tertiary structure by NMR and crystal structure determinations, overview Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 More the chitin-binding domain 2 belongs to the carbohydrate binding module 2 family of proteins Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 25 85 thermal stability of wild-type and mutant enzymes, overview Pyrococcus furiosus