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Literature summary extracted from
- Molecular cloning of the phospholipase D gene from Streptomyces sp. YU100 and its expression in Escherichia coli (2009), J. Microbiol., 47, 116-122.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.4.4 | recombinantly expressed in Escherchia coli as a His-tagged fusion protein | Streptomyces sp. |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.4 | Ca2+ | - |
Streptomyces sp. |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 54000 | - |
x * 54000, calculated from cDNA | Streptomyces sp. |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.4 | Streptomyces sp. | B0FZD9 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.4.4 | using Ni-NTA chromatography | Streptomyces sp. |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | additional information | - |
wild-type protein shows nearly the same enzymatic activity as the recombinant protein produced in Escherichia coli | Streptomyces sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.4 | phosphatidylcholine + H2O | - |
Streptomyces sp. | phosphatidate + choline | - |
? | |
| 3.1.4.4 | phosphatidylcholine + L-serine | - |
Streptomyces sp. | phosphatidyl-L-serine + choline | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.4.4 | ? | x * 54000, calculated from cDNA | Streptomyces sp. |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.4.4 | phospholipase D | - |
Streptomyces sp. |
| 3.1.4.4 | PLD | - |
Streptomyces sp. |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 35 | - |
transphosphatidylation reaction at | Streptomyces sp. |
| 3.1.4.4 | 37 | - |
hydrolytic reaction at | Streptomyces sp. |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 8 | - |
assay at | Streptomyces sp. |
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Release 2023.1 (January 2023)
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